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ATCC
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Image Search Results
Journal: Molecular Medicine
Article Title: TWEAK/Fn14 disrupts Th17/Treg balance and aggravates conjunctivitis by inhibiting the Nrf2/HO-1 pathway in allergic conjunctivitis mice
doi: 10.1186/s10020-024-01004-5
Figure Lengend Snippet: Primers of qRT-PCR
Article Snippet: Membranes were blocked by BSA (5%) for 1 h at room temperature and then cultured with primary antibodies against RORγt (ab207082, 1:1000; Abcam), FoxP3 (ab20034, 1:1000; Abcam),
Techniques: Sequencing
Journal: Molecular Medicine
Article Title: TWEAK/Fn14 disrupts Th17/Treg balance and aggravates conjunctivitis by inhibiting the Nrf2/HO-1 pathway in allergic conjunctivitis mice
doi: 10.1186/s10020-024-01004-5
Figure Lengend Snippet: Th17/Treg cell differentiation ratio and TWEAK/Fn14 signaling level in AC mice. ( A ) The state of mice ocular surface was observed by slit-lamp. ( B ) The clinical scores of mice were performed according to the status of eyelid, conjunctiva and cornea. ( C ) HE staining was utilized to evaluate the pathological changes of conjunctival tissue in mice. ( D ) The proportion of Th17 or Treg cells in spleen of mice was assessed by flow cytometry. ( E ) The levels of Th17 and Treg cytokines in mice spleen were evaluated by ELISA. ( F-H ) TWEAK and Fn14 levels in ocular conjunctival tissue were measured by qRT-PCR and WB. ( I ) IHC was used to observe the protein level of TWEAK and Fn14 in ocular conjunctival tissue. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Control
Article Snippet: Membranes were blocked by BSA (5%) for 1 h at room temperature and then cultured with primary antibodies against RORγt (ab207082, 1:1000; Abcam), FoxP3 (ab20034, 1:1000; Abcam),
Techniques: Cell Differentiation, Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Control
Journal: Molecular Medicine
Article Title: TWEAK/Fn14 disrupts Th17/Treg balance and aggravates conjunctivitis by inhibiting the Nrf2/HO-1 pathway in allergic conjunctivitis mice
doi: 10.1186/s10020-024-01004-5
Figure Lengend Snippet: TWEAK knockdown affected conjunctivitis in AC mice. ( A ) The effect of TWEAK knockdown on the state of mice ocular surface was observed under slit-lamp. ( B ) Clinical score of eyelid, conjunctiva and cornea to assess the effect of TWEAK knockdown in AC mice. ( C-D ) HE staining and TB staining were utilized to evaluate the effect of TWEAK knockdown on conjunctivitis in mice. ( E-G ) The levels of TWEAK and Fn14 in conjunctival tissue were detected by qRT-PCR, IHC and WB. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Con + AAV-shNC; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. AC + AAV-shNC; ns, no significant difference
Article Snippet: Membranes were blocked by BSA (5%) for 1 h at room temperature and then cultured with primary antibodies against RORγt (ab207082, 1:1000; Abcam), FoxP3 (ab20034, 1:1000; Abcam),
Techniques: Knockdown, Staining, Quantitative RT-PCR
Journal: Molecular Medicine
Article Title: TWEAK/Fn14 disrupts Th17/Treg balance and aggravates conjunctivitis by inhibiting the Nrf2/HO-1 pathway in allergic conjunctivitis mice
doi: 10.1186/s10020-024-01004-5
Figure Lengend Snippet: TWEAK regulated the Th17/Treg cell ratio in AC mice. ( A ) The effect of TWEAK knockdown on Th17 and Treg cell ratio in spleen of AC mice was observed by flow cytometry. ( B-C ) WB and IHC were employed to evaluate the expression levels of FoxP3 and RORγt in mice conjunctival tissue. ( D ) The effect of TWEAK knockdown on the levels of Th17 and Treg cytokines in mice spleen were evaluated by ELISA. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Con + AAV-shNC; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. AC + AAV-shNC; ns, no significant difference
Article Snippet: Membranes were blocked by BSA (5%) for 1 h at room temperature and then cultured with primary antibodies against RORγt (ab207082, 1:1000; Abcam), FoxP3 (ab20034, 1:1000; Abcam),
Techniques: Knockdown, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay
Journal: Molecular Medicine
Article Title: TWEAK/Fn14 disrupts Th17/Treg balance and aggravates conjunctivitis by inhibiting the Nrf2/HO-1 pathway in allergic conjunctivitis mice
doi: 10.1186/s10020-024-01004-5
Figure Lengend Snippet: TWEAK knockdown promoted Nrf2/HO-1 signaling pathway in AC mice. ( A ) qRT-PCR was utilized to measure the effect of TWEAK knockdown on Nrf2 and HO-1 mRNA levels in conjunctival tissue of mice. ( B-C ) The effect of TWEAK knockdown on protein levels of Nrf2 and HO-1 in conjunctival tissue of mice were detected by WB and IHC. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Con + AAV-shNC; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. AC + AAV-shNC; ns, no significant difference
Article Snippet: Membranes were blocked by BSA (5%) for 1 h at room temperature and then cultured with primary antibodies against RORγt (ab207082, 1:1000; Abcam), FoxP3 (ab20034, 1:1000; Abcam),
Techniques: Knockdown, Quantitative RT-PCR, Paraffin-embedded Immunohistochemistry
Journal: Molecular Medicine
Article Title: TWEAK/Fn14 disrupts Th17/Treg balance and aggravates conjunctivitis by inhibiting the Nrf2/HO-1 pathway in allergic conjunctivitis mice
doi: 10.1186/s10020-024-01004-5
Figure Lengend Snippet: Inhibition of Nrf2/HO-1 signaling pathway affected the improvement of conjunctivitis in AC mice from TWEAK knockdown. ( A ) The effect of Nrf2 inhibitor on ocular surface status was observed by slit-lamp in AC mice with TWEAK knockdown. ( B ) Clinical score of eyelid, conjunctiva and cornea to assess the effect of Nrf2 inhibitor in AC mice with TWEAK knockdown. ( C-D ) HE staining and TB staining were employed to evaluate the effect of Nrf2 inhibitor on conjunctivitis in AC mice with TWEAK knockdown. ( E-G ) The levels of Nrf2 and HO-1 in conjunctival tissue were detected by qRT-PCR, IHC and WB. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. AC + AAV-shNC; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. AC + AAV-shTWEAK
Article Snippet: Membranes were blocked by BSA (5%) for 1 h at room temperature and then cultured with primary antibodies against RORγt (ab207082, 1:1000; Abcam), FoxP3 (ab20034, 1:1000; Abcam),
Techniques: Inhibition, Knockdown, Staining, Quantitative RT-PCR
Journal: Molecular Medicine
Article Title: TWEAK/Fn14 disrupts Th17/Treg balance and aggravates conjunctivitis by inhibiting the Nrf2/HO-1 pathway in allergic conjunctivitis mice
doi: 10.1186/s10020-024-01004-5
Figure Lengend Snippet: Inhibition of Nrf2/HO-1 signaling pathway affected Th17/Treg cell ratio in AC mice with TWEAK knockdown. ( A ) The effect of Nrf2 inhibitor on Th17/Treg cell ratio in AC mice with TWEAK knockdown was assessed by flow cytometry. ( B-C ) WB and IHC assays were employed to evaluate the expression of FoxP3 and RORγt in conjunctival tissue of AC mice. ( D ) The levels of Th17 and Treg cytokines in mice spleen were detected by ELISA. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. AC + AAV-shNC; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. AC + AAV-shTWEAK
Article Snippet: Membranes were blocked by BSA (5%) for 1 h at room temperature and then cultured with primary antibodies against RORγt (ab207082, 1:1000; Abcam), FoxP3 (ab20034, 1:1000; Abcam),
Techniques: Inhibition, Knockdown, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay
Journal: iScience
Article Title: The strand exchange domain of tumor suppressor PALB2 is intrinsically disordered and promotes oligomerization-dependent DNA compaction
doi: 10.1016/j.isci.2024.111259
Figure Lengend Snippet:
Article Snippet: Na2HPO4 ,
Techniques: Virus, Recombinant, Protease Inhibitor, Mutagenesis, Software