Journal: RNA Biology

Article Title: A novel strategy for orthogonal genetic regulation on different RNA targeted loci simultaneously

doi: 10.1080/15476286.2022.2141507

Figure Lengend Snippet: Targeting miRNA to mediate specific gene up-regulating in human cells . (A) Schematic diagram of HpSGNi guided up-regulation in A549 cells heterologously expressing NES-FEN1. (B) The relative expression of EGFR in A549 cells transfected with FEN1-NES plus mis-hpDNA or not. mean ± SD. n = 2. * p value < 0.05. (C) The normalized expression of miRNA-21 in A549 cells transfected with FEN1-NES plus mis-hpDNA or not. mean ± SEM. n = 3 to 8. (D) The relative expression of EGFR and OCT4 in A549 cells transfected with FEN1-NES plus mis-hpDNA or not. n = 3. mean ± SD. * p value < 0.05.

Article Snippet: Primary antibodies included antibodies against OCT4 (Abcam, ab200834, 1:10,000), EGFR (Abclonal Technology, A11577, 1:1000) and β-actin (Abclonal Technology, AC026, 1:50,000).

Techniques: Expressing, Transfection

Journal: Cell reports

Article Title: BCL-xL inhibition potentiates cancer therapies by redirecting the outcome of p53 activation from senescence to apoptosis

doi: 10.1016/j.celrep.2022.111826

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: NVP-TAE 226 , Adooq Bioscience , A11507.

Techniques: Virus, Recombinant, Modification, Staining, Membrane, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Transfection, Saline, Protein Array, Control, Software