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8-Azidoadenosine is an adenosine analogue conjugated with an azido group which can be used for oligonucleotide labelling. The azido group reacts with a terminal alkyne via a click reaction, forming the labelled nucleotide containing a
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Image Search Results
Journal: Journal of Virology
Article Title: Double-Stranded RNA Adenosine Deaminase ADAR-1-Induced Hypermutated Genomes among Inactivated Seasonal Influenza and Live Attenuated Measles Virus Vaccines
doi: 10.1128/JVI.02138-10
Figure Lengend Snippet: ADAR editing of influenza virus A genomes from seasonal influenza virus vaccines. (A) 3DI-PCR of influenza virus A H1N1 vaccines (A5807-2 and A0667-1). Asterisks indicate the PCR products cloned and sequenced. The sizes of the 3DI-PCR fragments are 337 bp. (B) Some examples of hypermutated influenza virus genomes. (C) Mutation matrices of two hyperedited influenza virus sequences. The numbers below the matrices indicate the number of bases sequenced. (D) Frequency distribution of A→G editing per clone for the two vaccine lots. (E) Dinucleotide analysis in 5′ and 3′ of two edited influenza virus genomes. A χ2 analysis indicates dinucleotide frequencies deviating significantly from the expected values (*, P < 0.05).
Article Snippet: These vaccines were composites of the A/New Caledonia/20/99 H1N1, A/Wisconsin/67/2005 H3N2, and
Techniques: Clone Assay, Mutagenesis
Journal: Journal of Virology
Article Title: Double-Stranded RNA Adenosine Deaminase ADAR-1-Induced Hypermutated Genomes among Inactivated Seasonal Influenza and Live Attenuated Measles Virus Vaccines
doi: 10.1128/JVI.02138-10
Figure Lengend Snippet: Deep sequencing of influenza and measles virus genomes from commercial vaccines. (A) Mutation matrices of measles (NC70980) and influenza (A0667-1) virus genomes recovered at 95°C. (B) Frequency analysis of measles and influenza virus sequences in terms of the number of mutations per clone. (C) Hyperedited influenza virus A genome and antigenome sequences shown as differences with respect to the reference sequence. Only part of the 741-bp amplified fragment is shown.
Article Snippet: These vaccines were composites of the A/New Caledonia/20/99 H1N1, A/Wisconsin/67/2005 H3N2, and
Techniques: Sequencing, Mutagenesis, Amplification