A06431 Search Results


anti-atp1a1 antibody  (ABclonal Biotechnology)
90
ABclonal Biotechnology anti-atp1a1 antibody
a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. <t>ATP1A1</t> was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.
Anti Atp1a1 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-atp1a1 antibody - by Bioz Stars, 2026-02
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detection kit a0641-1  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
90
Nanjing Jiancheng Bioengineering Research Institute Co Ltd detection kit a0641-1
a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. <t>ATP1A1</t> was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.
Detection Kit A0641 1, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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detection kit a0641-1 - by Bioz Stars, 2026-02
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primary antibody reck  (Boster Bio)
88
Boster Bio primary antibody reck
a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. <t>ATP1A1</t> was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.
Primary Antibody Reck, supplied by Boster Bio, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lyz a0641  (ABclonal Biotechnology)
90
ABclonal Biotechnology lyz a0641
a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. <t>ATP1A1</t> was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.
Lyz A0641, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lyz a0641 - by Bioz Stars, 2026-02
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anti-na+/k + -atpase α (a0643)  (ABclonal Biotechnology)
90
ABclonal Biotechnology anti-na+/k + -atpase α (a0643)
a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. <t>ATP1A1</t> was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.
Anti Na+/K + Atpase α (A0643), supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody cat no. as014  (ABclonal Biotechnology)
90
ABclonal Biotechnology antibody cat no. as014
a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. <t>ATP1A1</t> was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.
Antibody Cat No. As014, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti chrna5 antibody  (Boster Bio)
90
Boster Bio anti chrna5 antibody
a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. <t>ATP1A1</t> was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.
Anti Chrna5 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cav 1  (Boster Bio)
92
Boster Bio cav 1
a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. <t>ATP1A1</t> was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.
Cav 1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydrogen peroxide (h2o2) kits  (Jiancheng Inc)
90
Jiancheng Inc hydrogen peroxide (h2o2) kits
a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. <t>ATP1A1</t> was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.
Hydrogen Peroxide (H2o2) Kits, supplied by Jiancheng Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atp1a  (ABclonal Biotechnology)
90
ABclonal Biotechnology atp1a
a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. <t>ATP1A1</t> was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.
Atp1a, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endogenous peroxidase activity  (Boster Bio)
90
Boster Bio endogenous peroxidase activity
a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. <t>ATP1A1</t> was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.
Endogenous Peroxidase Activity, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-reck antibody picoband  (Boster Bio)
90
Boster Bio anti-reck antibody picoband
a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. <t>ATP1A1</t> was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.
Anti Reck Antibody Picoband, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. ATP1A1 was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.

Journal: bioRxiv

Article Title: Hypercholesterolemia risk associated GPR146 is an orphan G-protein coupled receptor that regulates blood cholesterol level in human and mouse

doi: 10.1101/2020.01.09.901041

Figure Lengend Snippet: a , Tissue distribution of gpr146 in mice. C57BL/6J wild type mice were fast overnight and indicated tissues were collected for RT-PCR analysis. Expression levels were normalized to liver which was set to 1 (N=5, male, 8 weeks). BAT, brown adipose tissue; WAT, white adipose tissue; SM, skeletal muscle; SI, small intestine. b . Gpr146 is specifically expressed in hepatocytes of mouse liver. Primary hepatocytes and non-hepatocytes are separated and subjected to RT-PCR analysis as described in Methods. Albumin and Apob are hepatocytes marker genes, F4/80 is a macrophage marker gene. c . Gpr146 is located in membrane fraction. Flag tagged Gpr146 was expressed in 293T cells by transient transfection. Cytosol, membrane and nuclear fractions were isolated as described in Methods. Gpr146 was detected with anti-Flag antibody. Actin, Calnexin and Lamin B1 were used as markers for each fraction. d , GPR146 is located on plasma membrane. GPR146 tagged with Flag was expressed in 293T cells by transient transfection. Cell surface fraction was isolated with biotinylation in part of the cells as described in Methods. GPR146 was detected with an anti-GPR146 polyclonal antibody. ATP1A1 was used a cell surface marker. e , GPR146 responses to 3 kD filtered serum and activates cAMP response element (CRE) activity. HEK293 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of fetal bovine serum (FBS) that has passed through the 3 kD cut-off filter. Luciferase activities were measured accordingly. f , GPR146 responses to heat-inactivated serum and actives CRE activity through PKA pathway. HepG2 cells expressing empty vector or GPR146 together with CRE-Luciferase reporter were treated with indicated amount of FBS that has passed through the 3 kD cut-off filter and further heat-inactivated by boiling. Part of the cells was treated in the presence of PKA inhibitor H-89 (10 μm). All data are expressed as means ± SEM and p values were calculated using Student’s test (*** p <0.001). All experiments were repeated at least twice with similar results.

Article Snippet: The following antibodies were used in this study: anti-Flag antibody (Medical & Biological Laboratories, PM020); anti-calnexin antibody (Proteintech, 10427-2-AP); anti-Actin antibody (Proteintech, 20536-1-AP), anti-Lamin B1 antibody (Proteintech, 20536-1-AP), anti-GPR146 antibody (CUSABIO,CSB-PA006863), anti-ATP1A1 antibody (Abclonal, A0643), anti-ApoB antibody and anti-ApoA1 antibody (Proteintech, 14427-1-AP).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Marker, Transfection, Isolation, Activity Assay, Plasmid Preparation, Luciferase