Journal: PeerJ

Article Title: Proteomic analysis reveals some common proteins in the kidney stone matrix

doi: 10.7717/peerj.11872

Figure Lengend Snippet: Basic information of included studies. Detailed information of the 19 studies we included in our research.

Article Snippet: Slices of the tissue were incubated with anti-S100A8 antibody (ab92331; Abcam, Cambridge, UK), anti-S100A9 antibody (ab92507; Abcam, Cambridge, UK), anti-uromodulin antibody (A01303-2; Boster, Selangor, Malaysia), anti-OPN (ab8448; Abcam, Cambridge, UK), and anti-albumin antibody (A1363; Abclonal, Wuhan, China) for immunohistochemistry.

Techniques: Western Blot, Molecular Weight, Methylation, Mass Spectrometry, Binding Assay, Calcium Carbonate, Clinical Proteomics, Coagulation

Journal: PeerJ

Article Title: Proteomic analysis reveals some common proteins in the kidney stone matrix

doi: 10.7717/peerj.11872

Figure Lengend Snippet: Biological information of the 20 most common proteins in stone matrix. Biological information of the 20 most common proteins in stone matrix (from the Human Protein Altas).

Article Snippet: Slices of the tissue were incubated with anti-S100A8 antibody (ab92331; Abcam, Cambridge, UK), anti-S100A9 antibody (ab92507; Abcam, Cambridge, UK), anti-uromodulin antibody (A01303-2; Boster, Selangor, Malaysia), anti-OPN (ab8448; Abcam, Cambridge, UK), and anti-albumin antibody (A1363; Abclonal, Wuhan, China) for immunohistochemistry.

Techniques: Expressing, Chemotaxis Assay, Mutagenesis, Coagulation

Journal: PeerJ

Article Title: Proteomic analysis reveals some common proteins in the kidney stone matrix

doi: 10.7717/peerj.11872

Figure Lengend Snippet: The renal expression of S100A8, S100A9, and osteopontin were increased, while uromodulin was decreased in kidney stone patients. Albumin was rarely expressed in kidney and there was no significant difference between healthy controls and kidney stone patients; * P < 0.05 vs Control; ** P < 0.01 vs Control.

Article Snippet: Slices of the tissue were incubated with anti-S100A8 antibody (ab92331; Abcam, Cambridge, UK), anti-S100A9 antibody (ab92507; Abcam, Cambridge, UK), anti-uromodulin antibody (A01303-2; Boster, Selangor, Malaysia), anti-OPN (ab8448; Abcam, Cambridge, UK), and anti-albumin antibody (A1363; Abclonal, Wuhan, China) for immunohistochemistry.

Techniques: Expressing, Control

Journal: International journal of oncology

Article Title: Metastatic cancer cells compensate for low energy supplies in hostile microenvironments with bioenergetic adaptation and metabolic reprogramming.

doi: 10.3892/ijo.2018.4582

Figure Lengend Snippet: Figure 2. Expression levels of glycolysis regulation proteins in SW480 and SW620 cells with different metabolic types. (A and B) Representative western blotting images of HIF-1α, HK1, HK2 and GLUT1 protein expressions in (A) SW480 and (B) SW620 cells cultured in different media. (C-F) Protein expression levels from (A) and (B) were quantified using Image Lab analysis software; the relative expression levels of HIF-1α, HK1, HK2 and GLUT1 were calculated as a ratio of OXPHOS; β-actin was used as the loading control. (G) Western blot analysis of HK1 and HK2 protein expression levels in SW480 and SW620 cells treated with Oligomycin A (1 µM) for 0-48 h. (G) Representative western blots indicating the expression of HK1 and HK2 in SW480 and SW620 cells at different treatment times. (H and I) Protein expression levels from (G) were quantified using Image Lab analysis software, and relative expression levels of (H) HK1 and (I) HK2 were calculated as a ratio of 0 h; β-actin was used as a loading control. Data are presented as the mean ± standard deviation; n=3; **P<0.01 and ***P<0.001 vs. SW480. GLUT1, glucose transporter type 1; HIF-1α, hypoxia-inducible factor; HK, hexokinase; NOR, normal; GLY, glycolysis; OXPHOS, oxidative phosphorylation.

Article Snippet: Mouse anti-HK1 (cat. no. BM1910) and mouse anti-human HK2 (cat. no. BM1911) antibodies were purchased from Boster Biological Technology (Pleasanton, CA, USA).

Techniques: Expressing, Western Blot, Cell Culture, Software, Control, Standard Deviation, Phospho-proteomics

Journal: International journal of oncology

Article Title: Metastatic cancer cells compensate for low energy supplies in hostile microenvironments with bioenergetic adaptation and metabolic reprogramming.

doi: 10.3892/ijo.2018.4582

Figure Lengend Snippet: Figure 6. RT-qPCR analysis of SW480 and SW620 cells in different culture microenvironments. (A-C) RT-qPCR analysis of the relative mRNA expression levels of HK1, HK2, HIF-1α, CDH1, FN1, VIM, CD133, SNAI1, SNAI2, ZEB1, CDH2 and TWIST1 in SW480 and SW620 cells cultured in different media conditions; mRNA expressions were normalized to β-actin. (D) SW620/SW480 mRNA expression ratios in different types of metabolism. Data are presented as the mean ± standard deviation; n=3; *P<0.05, **P<0.01 and ***P<0.001 vs. SW480. CDH1, Epithelial cadherin; FN1, fibronectin; HIF-1α, hypoxia-inducible factor; HK, hexokinase; Oligo A, Oligomycin A; OXPHOS, oxidative phosphorylation; SNAI, snail family transcriptional repressor; VIM, vimentin; ZEB, Zinc-finger E-box-binding homeobox; NOR, normal; GLY, glycolysis; OXPH, oxidative phosphorylation.

Article Snippet: Mouse anti-HK1 (cat. no. BM1910) and mouse anti-human HK2 (cat. no. BM1911) antibodies were purchased from Boster Biological Technology (Pleasanton, CA, USA).

Techniques: Quantitative RT-PCR, Expressing, Cell Culture, Standard Deviation, Phospho-proteomics, Binding Assay

Journal: International journal of oncology

Article Title: Metastatic cancer cells compensate for low energy supplies in hostile microenvironments with bioenergetic adaptation and metabolic reprogramming.

doi: 10.3892/ijo.2018.4582

Figure Lengend Snippet: Figure 7. mRNA levels in SW480 and SW620 cells treated with Oligo A over time. Reverse transcription-quantitative polymerase chain reaction analysis of mRNA expression levels of HK1, HK2, HIF-1α, CDH1, CDH2, ZEB1, SNAI1, SNAI2, TWIST1, CD133, VIM and FN in SW480 and SW620 cells treated with Oligomycin A (1 µM) for 0-48 h. mRNA expression levels were normalized to β-actin. Data are presented as the mean ± standard deviation; n=3; **P<0.01; ***P<0.001 vs. SW480. CDH1, epithelial cadherin; CDH2, Neural-cadherin; FN1, fibronectin; HIF-1α, hypoxia-inducible factor; HK, hexokinase; SNAI, snail family transcriptional repressor; VIM, vimentin; ZEB, Zinc-finger E-box-binding homeobox.

Article Snippet: Mouse anti-HK1 (cat. no. BM1910) and mouse anti-human HK2 (cat. no. BM1911) antibodies were purchased from Boster Biological Technology (Pleasanton, CA, USA).

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Standard Deviation, Binding Assay