Journal: Nanomaterials

Article Title: Exosomal Surface Protein Detection with Quantum Dots and Immunomagnetic Capture for Cancer Detection

doi: 10.3390/nano11071853

Figure Lengend Snippet: Schematic of the QD-based EXO assay ( a ), and characterization of EXOs ( b ), and QDs ( c – e ). EXOs were captured from biofluids with MB via CD81 monoclonal antibodies. Targeted surface cancer marker was recognized with primary antibody and then detected with secondary antibody-conjugated QD655. Signals were measured with fluorescence spectroscopy to quantify the QDs and correspondingly the surface protein markers on EXOs. ( b ) SEM image of plasma exosomes from a BC patient. ( c ) Absorption spectrum and ( d ) emission spectrum of IgG-QD655. ( e ) DLS characterization of the hydrodynamic size of IgG-QD655 and MB.

Article Snippet: Anti-rabbit CD81 antibody was purchased from Boster Biological Technology (Pleasanton, CA, USA).

Techniques: Marker, Fluorescence, Spectroscopy