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  • 99
    Bethyl rabbit anti 53bp1 antibody affinity purified
    Rabbit Anti 53bp1 Antibody Affinity Purified, supplied by Bethyl, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bethyl rabbit anti tfeb antibody affinity purified
    Acinar cell–specific <t>TFEB</t> KO mice develop severe pancreatitis with the Lieber-DeCarli liquid diet but not with the chow diet. BAC-Ela-Cre-; TFEB f/f (TFEB WT) and BAC-Ela-Cre+; TFEB f/f (TFEB KO) mice were injected with tamoxifen (75 mg/kg) once a day for consecutive 5 days, then fed with the liquid control diet or chow diet. ( A ) Representative immunofluorescence images of LAMP1 (red) and TAP (green) from TFEB WT and TFEB KO mice fed with the liquid control diet. Boxed area was enlarged and shown on the right. The arrowhead shows colocalized puncta of LAMP1 and TAP; the arrow denotes TAP-positive puncta. Scale bar = 20 μm. ( B ) Representative hematoxylin and eosin (H E), Sirius red, and <t>CK19</t> staining images of liquid control diet–fed mouse pancreas are shown. ( C ) H E staining images of chow diet–fed mouse pancreas are shown. ( D ) Total pancreatic lysates from chow diet–fed TFEB WT mice and TFEB KO mice were subjected to Western blot analysis.
    Rabbit Anti Tfeb Antibody Affinity Purified, supplied by Bethyl, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti tfeb antibody affinity purified/product/Bethyl
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti tfeb antibody affinity purified - by Bioz Stars, 2022-08
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    99
    Bethyl rabbit anti smc1 antibody affinity purified
    Acinar cell–specific <t>TFEB</t> KO mice develop severe pancreatitis with the Lieber-DeCarli liquid diet but not with the chow diet. BAC-Ela-Cre-; TFEB f/f (TFEB WT) and BAC-Ela-Cre+; TFEB f/f (TFEB KO) mice were injected with tamoxifen (75 mg/kg) once a day for consecutive 5 days, then fed with the liquid control diet or chow diet. ( A ) Representative immunofluorescence images of LAMP1 (red) and TAP (green) from TFEB WT and TFEB KO mice fed with the liquid control diet. Boxed area was enlarged and shown on the right. The arrowhead shows colocalized puncta of LAMP1 and TAP; the arrow denotes TAP-positive puncta. Scale bar = 20 μm. ( B ) Representative hematoxylin and eosin (H E), Sirius red, and <t>CK19</t> staining images of liquid control diet–fed mouse pancreas are shown. ( C ) H E staining images of chow diet–fed mouse pancreas are shown. ( D ) Total pancreatic lysates from chow diet–fed TFEB WT mice and TFEB KO mice were subjected to Western blot analysis.
    Rabbit Anti Smc1 Antibody Affinity Purified, supplied by Bethyl, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti smc1 antibody affinity purified/product/Bethyl
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti smc1 antibody affinity purified - by Bioz Stars, 2022-08
    99/100 stars
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    86
    Miltenyi Biotec macs multistand
    Acinar cell–specific <t>TFEB</t> KO mice develop severe pancreatitis with the Lieber-DeCarli liquid diet but not with the chow diet. BAC-Ela-Cre-; TFEB f/f (TFEB WT) and BAC-Ela-Cre+; TFEB f/f (TFEB KO) mice were injected with tamoxifen (75 mg/kg) once a day for consecutive 5 days, then fed with the liquid control diet or chow diet. ( A ) Representative immunofluorescence images of LAMP1 (red) and TAP (green) from TFEB WT and TFEB KO mice fed with the liquid control diet. Boxed area was enlarged and shown on the right. The arrowhead shows colocalized puncta of LAMP1 and TAP; the arrow denotes TAP-positive puncta. Scale bar = 20 μm. ( B ) Representative hematoxylin and eosin (H E), Sirius red, and <t>CK19</t> staining images of liquid control diet–fed mouse pancreas are shown. ( C ) H E staining images of chow diet–fed mouse pancreas are shown. ( D ) Total pancreatic lysates from chow diet–fed TFEB WT mice and TFEB KO mice were subjected to Western blot analysis.
    Macs Multistand, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Acinar cell–specific TFEB KO mice develop severe pancreatitis with the Lieber-DeCarli liquid diet but not with the chow diet. BAC-Ela-Cre-; TFEB f/f (TFEB WT) and BAC-Ela-Cre+; TFEB f/f (TFEB KO) mice were injected with tamoxifen (75 mg/kg) once a day for consecutive 5 days, then fed with the liquid control diet or chow diet. ( A ) Representative immunofluorescence images of LAMP1 (red) and TAP (green) from TFEB WT and TFEB KO mice fed with the liquid control diet. Boxed area was enlarged and shown on the right. The arrowhead shows colocalized puncta of LAMP1 and TAP; the arrow denotes TAP-positive puncta. Scale bar = 20 μm. ( B ) Representative hematoxylin and eosin (H E), Sirius red, and CK19 staining images of liquid control diet–fed mouse pancreas are shown. ( C ) H E staining images of chow diet–fed mouse pancreas are shown. ( D ) Total pancreatic lysates from chow diet–fed TFEB WT mice and TFEB KO mice were subjected to Western blot analysis.

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Critical Role of TFEB-Mediated Lysosomal Biogenesis in Alcohol-Induced Pancreatitis in Mice and Humans

    doi: 10.1016/j.jcmgh.2020.01.008

    Figure Lengend Snippet: Acinar cell–specific TFEB KO mice develop severe pancreatitis with the Lieber-DeCarli liquid diet but not with the chow diet. BAC-Ela-Cre-; TFEB f/f (TFEB WT) and BAC-Ela-Cre+; TFEB f/f (TFEB KO) mice were injected with tamoxifen (75 mg/kg) once a day for consecutive 5 days, then fed with the liquid control diet or chow diet. ( A ) Representative immunofluorescence images of LAMP1 (red) and TAP (green) from TFEB WT and TFEB KO mice fed with the liquid control diet. Boxed area was enlarged and shown on the right. The arrowhead shows colocalized puncta of LAMP1 and TAP; the arrow denotes TAP-positive puncta. Scale bar = 20 μm. ( B ) Representative hematoxylin and eosin (H E), Sirius red, and CK19 staining images of liquid control diet–fed mouse pancreas are shown. ( C ) H E staining images of chow diet–fed mouse pancreas are shown. ( D ) Total pancreatic lysates from chow diet–fed TFEB WT mice and TFEB KO mice were subjected to Western blot analysis.

    Article Snippet: Antibodies The antibodies used for this study were AMYLASE (A8273; Sigma-Aldrich), Atg5 (PM050, MBL, Woburn, MA), phos-TFEB (ABE1971; Millipore, Burlington, MA), TFEB (A303-673A; Bethyl Laboratories, Montgomery, TX), CK19 (TROMA-III, Developmental Studies Hybridoma Bank, Iowa City, IA), GAPDH (2118; Cell Signaling Technology, Danvers, MA), p62 (H00008878-M01; Abnova, Taipei, Taiwan), phos-4EBP1 (9451; Cell Signaling Technology), 4EBP1 (9452; Cell Signaling Technology), phos-MAPK (9101; Cell Signaling Technology), MAPK (9102; Cell Signaling Technology), phos-S6-ribosomal protein (4858; Cell Signaling Technology), S6-ribosomal protein (2217; Cell Signaling Technology), GFP (sc-9996; Santa Cruz Biotechnology, Dallas, TX), LAMIN A/C (2032; Cell Signaling Technology), LAMP1 (1D4B and H4A3; Developmental Studies Hybridoma Bank, Iowa City, IA), LAMP2 (ABL-93 and H4A4; Developmental Studies Hybridoma Bank), and PGC1α (PAB12061; Abnova).

    Techniques: Mouse Assay, BAC Assay, Injection, Immunofluorescence, Staining, Western Blot