956-02-5 Search Results


3mb pp1  (MedChemExpress)
90
MedChemExpress 3mb pp1
A, B MCF10A or AS‐CDC7 cells were either mock‐treated or treated with 10 μM XL413 for 30 min, at which point 4 mM HU was added and cells further incubated for the indicated times. Whole‐cell extracts were then analysed by Western blotting with the indicated antibodies. Data are representative of at least two independent experiments. C AS‐CDC7 cells were either mock‐treated or treated with 10 μM <t>3MB‐PP1,</t> 4 mM HU or both for 24 h before performing neutral comet assays. Representative images of cells are shown. Scale bar = 100 μm. In the dot plots, ˜400 comets per each condition were analysed, means are indicated with red lines, and their values are shown above the plots. Data are from two independent experiments. Source data are available online for this figure.
3mb Pp1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3mb pp1 - by Bioz Stars, 2026-09
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A, B MCF10A or AS‐CDC7 cells were either mock‐treated or treated with 10 μM XL413 for 30 min, at which point 4 mM HU was added and cells further incubated for the indicated times. Whole‐cell extracts were then analysed by Western blotting with the indicated antibodies. Data are representative of at least two independent experiments. C AS‐CDC7 cells were either mock‐treated or treated with 10 μM 3MB‐PP1, 4 mM HU or both for 24 h before performing neutral comet assays. Representative images of cells are shown. Scale bar = 100 μm. In the dot plots, ˜400 comets per each condition were analysed, means are indicated with red lines, and their values are shown above the plots. Data are from two independent experiments. Source data are available online for this figure.

Journal: EMBO Reports

Article Title: CDC7 kinase promotes MRE11 fork processing, modulating fork speed and chromosomal breakage

doi: 10.15252/embr.201948920

Figure Lengend Snippet: A, B MCF10A or AS‐CDC7 cells were either mock‐treated or treated with 10 μM XL413 for 30 min, at which point 4 mM HU was added and cells further incubated for the indicated times. Whole‐cell extracts were then analysed by Western blotting with the indicated antibodies. Data are representative of at least two independent experiments. C AS‐CDC7 cells were either mock‐treated or treated with 10 μM 3MB‐PP1, 4 mM HU or both for 24 h before performing neutral comet assays. Representative images of cells are shown. Scale bar = 100 μm. In the dot plots, ˜400 comets per each condition were analysed, means are indicated with red lines, and their values are shown above the plots. Data are from two independent experiments. Source data are available online for this figure.

Article Snippet: PHA‐767491 (3140; TOCRIS Bioscience), XL413 (synthesized in‐house) and 3MB‐PP1 (Cayman Chemical Co., 17860) were all used at a concentration of 10 μM, while AZD6738 and mirin (HY‐19323 and HY‐19959; MedChemExpress) were used at 5 and 50 μM, respectively.

Techniques: Incubation, Western Blot

U2OS or AS‐CDC7 cells were transfected with control (siCon) or BRCA2 (siBRCA2)‐targeting siRNAs and then either mock‐treated or treated with 4 mM HU for 5 h in the presence or absence of 10 μM XL413 or 3MB‐PP1, respectively. HU was then washed off and nocodazole was added to the medium in order to complete one round of DNA synthesis. Metaphase spreads were prepared and analysed. A Representative metaphase spread from BRCA2‐depleted U2OS cells treated with HU. Scale bar = 5 μm. Arrows indicate the chromosomes enlarged in the insets, two of which show chromatid breaks (*). B, C The graphs show the average number of chromatid breaks per spread. In each experiment, 30 chromosome spreads for each condition were analysed and three independent experiments were performed. Error bars represent SEM. Statistical significance was assessed by Student's t ‐test (** P ˂ 0.01, *** P < 0.001). D, E U2OS and AS‐CDC7 cells were transfected with control (siCon) or BRCA2 (siBRCA2)‐targeting siRNAs. Forty‐eight hours post‐transfection, cells were either mock‐treated or treated with 10 μM XL413 or 10 μM 3MB‐PP1, respectively, for 24 h with nocodazole added for the last 16 h of the experiment. The graph shows the average number of chromatid breaks per spread. In each experiment, 30 chromosome spreads for each condition were analysed and three independent experiments were performed. Error bars represent SEM. Statistical significance was assessed by Student's t ‐test (* P < 0.05, *** P < 0.001). Source data are available online for this figure.

Journal: EMBO Reports

Article Title: CDC7 kinase promotes MRE11 fork processing, modulating fork speed and chromosomal breakage

doi: 10.15252/embr.201948920

Figure Lengend Snippet: U2OS or AS‐CDC7 cells were transfected with control (siCon) or BRCA2 (siBRCA2)‐targeting siRNAs and then either mock‐treated or treated with 4 mM HU for 5 h in the presence or absence of 10 μM XL413 or 3MB‐PP1, respectively. HU was then washed off and nocodazole was added to the medium in order to complete one round of DNA synthesis. Metaphase spreads were prepared and analysed. A Representative metaphase spread from BRCA2‐depleted U2OS cells treated with HU. Scale bar = 5 μm. Arrows indicate the chromosomes enlarged in the insets, two of which show chromatid breaks (*). B, C The graphs show the average number of chromatid breaks per spread. In each experiment, 30 chromosome spreads for each condition were analysed and three independent experiments were performed. Error bars represent SEM. Statistical significance was assessed by Student's t ‐test (** P ˂ 0.01, *** P < 0.001). D, E U2OS and AS‐CDC7 cells were transfected with control (siCon) or BRCA2 (siBRCA2)‐targeting siRNAs. Forty‐eight hours post‐transfection, cells were either mock‐treated or treated with 10 μM XL413 or 10 μM 3MB‐PP1, respectively, for 24 h with nocodazole added for the last 16 h of the experiment. The graph shows the average number of chromatid breaks per spread. In each experiment, 30 chromosome spreads for each condition were analysed and three independent experiments were performed. Error bars represent SEM. Statistical significance was assessed by Student's t ‐test (* P < 0.05, *** P < 0.001). Source data are available online for this figure.

Article Snippet: PHA‐767491 (3140; TOCRIS Bioscience), XL413 (synthesized in‐house) and 3MB‐PP1 (Cayman Chemical Co., 17860) were all used at a concentration of 10 μM, while AZD6738 and mirin (HY‐19323 and HY‐19959; MedChemExpress) were used at 5 and 50 μM, respectively.

Techniques: Transfection, Control, DNA Synthesis