9310-05a Search Results


human urethral epithelial cells  (Cell Applications Inc)
93
Cell Applications Inc human urethral epithelial cells
Phase-dependent SHED-EV signatures and OMF proliferation in a urethra-mimetic paracrine model. ( A ) Focused heat map of miRNAs linked to <t>epithelial</t> regeneration and fibrosis modulation (miR-21, miR-181 family, miR-31, miR-146 family, miR-486, miR-192, miR-339, miR-99/100, miR-205, let-7 family) in SHED-EVs collected during the logarithmic growth phase (SHED-EV-L) or the stationary phase (SHED-EV-S). Signals were background-corrected, globally normalized, and median-centered; red indicates higher and blue lower expression relative to the median. ( B – D ) Proliferative responses of human oral mucosa fibroblasts (OMFs) indirectly co-cultured with urethral epithelial cells (UECs) in a horizontal plate with laterally linked chambers separated by 1.2 µm porous barriers (no direct contact). EVs were added at doses normalized by nanoparticle tracking analysis. Time-lapse phase-contrast images were acquired in the incubator every 60 min and confluence was quantified by automated segmentation. Data are mean ± SEM (standard error of the mean), n = 3 independent wells per group. ( B ) Confluence at 24, 48, 72, and 96 h for vehicle control, EV-L, and EV-S. Statistical analysis: two-way ANOVA followed by Tukey’s multiple-comparison test (GraphPad Prism 9, San Diego, CA, USA); significance threshold p < 0.05. Symbols: ** p < 0.01 vs. control; ## p < 0.01 vs. SHED-EV-L at the same time point. ( C ) Interval growth rates expressed as confluence slopes over 24–48, 48–72, and 72–96 h (%/h). ( D ) Representative fields at 48, 72, and 96 h for each group, captured at identical magnification; segmentation masks are shown in blue.
Human Urethral Epithelial Cells, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Phase-dependent SHED-EV signatures and OMF proliferation in a urethra-mimetic paracrine model. ( A ) Focused heat map of miRNAs linked to epithelial regeneration and fibrosis modulation (miR-21, miR-181 family, miR-31, miR-146 family, miR-486, miR-192, miR-339, miR-99/100, miR-205, let-7 family) in SHED-EVs collected during the logarithmic growth phase (SHED-EV-L) or the stationary phase (SHED-EV-S). Signals were background-corrected, globally normalized, and median-centered; red indicates higher and blue lower expression relative to the median. ( B – D ) Proliferative responses of human oral mucosa fibroblasts (OMFs) indirectly co-cultured with urethral epithelial cells (UECs) in a horizontal plate with laterally linked chambers separated by 1.2 µm porous barriers (no direct contact). EVs were added at doses normalized by nanoparticle tracking analysis. Time-lapse phase-contrast images were acquired in the incubator every 60 min and confluence was quantified by automated segmentation. Data are mean ± SEM (standard error of the mean), n = 3 independent wells per group. ( B ) Confluence at 24, 48, 72, and 96 h for vehicle control, EV-L, and EV-S. Statistical analysis: two-way ANOVA followed by Tukey’s multiple-comparison test (GraphPad Prism 9, San Diego, CA, USA); significance threshold p < 0.05. Symbols: ** p < 0.01 vs. control; ## p < 0.01 vs. SHED-EV-L at the same time point. ( C ) Interval growth rates expressed as confluence slopes over 24–48, 48–72, and 72–96 h (%/h). ( D ) Representative fields at 48, 72, and 96 h for each group, captured at identical magnification; segmentation masks are shown in blue.

Journal: International Journal of Molecular Sciences

Article Title: Culture Growth Phase-Dependent Influence of Extracellular Vesicles Derived from Stem Cells from Human Exfoliated Deciduous Teeth on Oral Mucosa Cells Proliferation in Paracrine Co-Culture with Urethral Epithelium: Implication for Urethral Reconstruction

doi: 10.3390/ijms27010314

Figure Lengend Snippet: Phase-dependent SHED-EV signatures and OMF proliferation in a urethra-mimetic paracrine model. ( A ) Focused heat map of miRNAs linked to epithelial regeneration and fibrosis modulation (miR-21, miR-181 family, miR-31, miR-146 family, miR-486, miR-192, miR-339, miR-99/100, miR-205, let-7 family) in SHED-EVs collected during the logarithmic growth phase (SHED-EV-L) or the stationary phase (SHED-EV-S). Signals were background-corrected, globally normalized, and median-centered; red indicates higher and blue lower expression relative to the median. ( B – D ) Proliferative responses of human oral mucosa fibroblasts (OMFs) indirectly co-cultured with urethral epithelial cells (UECs) in a horizontal plate with laterally linked chambers separated by 1.2 µm porous barriers (no direct contact). EVs were added at doses normalized by nanoparticle tracking analysis. Time-lapse phase-contrast images were acquired in the incubator every 60 min and confluence was quantified by automated segmentation. Data are mean ± SEM (standard error of the mean), n = 3 independent wells per group. ( B ) Confluence at 24, 48, 72, and 96 h for vehicle control, EV-L, and EV-S. Statistical analysis: two-way ANOVA followed by Tukey’s multiple-comparison test (GraphPad Prism 9, San Diego, CA, USA); significance threshold p < 0.05. Symbols: ** p < 0.01 vs. control; ## p < 0.01 vs. SHED-EV-L at the same time point. ( C ) Interval growth rates expressed as confluence slopes over 24–48, 48–72, and 72–96 h (%/h). ( D ) Representative fields at 48, 72, and 96 h for each group, captured at identical magnification; segmentation masks are shown in blue.

Article Snippet: Primary human oral mucosa fibroblasts (OMFs; hOMF100, CellResearch Corporation; distributor Cosmo Bio, Tokyo, Japan; received at P3) and human urethral epithelial cells (UECs; HUEpC, Cell Applications, Cat. 9310-05a) were expanded according to the suppliers’ instructions at 37 °C with 5% CO 2 and were used at passages ≤ 6.

Techniques: Expressing, Cell Culture, Control, Comparison

Horizontal indirect co-culture of oral mucosa fibroblasts with urethral epithelial cells and SHED-derived extracellular vesicles. Schematic of the horizontal indirect co-culture used for paracrine assays. Three chambers are linked in series by lateral microchannels separated by 1.2 µm porous barriers that block cell migration while permitting diffusion of soluble factors and extracellular vesicles (EVs). SHED-EV-L or SHED-EV-S are added to the left reservoir; oral mucosa fibroblasts (OMFs) are seeded in the central chamber; urethral epithelial cells (UECs) are seeded in the right chamber. A connector allows three or more modules to be linked. Arrows indicate bidirectional lateral diffusion and the absence of direct OMF–UEC contact. Cultures were imaged in the incubator every 60 min for up to 96 h; OMF proliferation was quantified as confluence and interval slopes.

Journal: International Journal of Molecular Sciences

Article Title: Culture Growth Phase-Dependent Influence of Extracellular Vesicles Derived from Stem Cells from Human Exfoliated Deciduous Teeth on Oral Mucosa Cells Proliferation in Paracrine Co-Culture with Urethral Epithelium: Implication for Urethral Reconstruction

doi: 10.3390/ijms27010314

Figure Lengend Snippet: Horizontal indirect co-culture of oral mucosa fibroblasts with urethral epithelial cells and SHED-derived extracellular vesicles. Schematic of the horizontal indirect co-culture used for paracrine assays. Three chambers are linked in series by lateral microchannels separated by 1.2 µm porous barriers that block cell migration while permitting diffusion of soluble factors and extracellular vesicles (EVs). SHED-EV-L or SHED-EV-S are added to the left reservoir; oral mucosa fibroblasts (OMFs) are seeded in the central chamber; urethral epithelial cells (UECs) are seeded in the right chamber. A connector allows three or more modules to be linked. Arrows indicate bidirectional lateral diffusion and the absence of direct OMF–UEC contact. Cultures were imaged in the incubator every 60 min for up to 96 h; OMF proliferation was quantified as confluence and interval slopes.

Article Snippet: Primary human oral mucosa fibroblasts (OMFs; hOMF100, CellResearch Corporation; distributor Cosmo Bio, Tokyo, Japan; received at P3) and human urethral epithelial cells (UECs; HUEpC, Cell Applications, Cat. 9310-05a) were expanded according to the suppliers’ instructions at 37 °C with 5% CO 2 and were used at passages ≤ 6.

Techniques: Co-Culture Assay, Derivative Assay, Blocking Assay, Migration, Diffusion-based Assay