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90
ATCC a pleuropneumoniae field
Immunoblot of whole-cell, proteinase K-treated preparations of A. <t>pleuropneumoniae</t> reference strain S4074 (lane 1) and strain FMV 91-6514 (lane 2). The immunoblot was probed with a monoclonal antibody against A. pleuropneumoniae serotype 1 O-antigen. Molecular mass markers (in kilodaltons) are indicated on the left.
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Immunoblot of whole-cell, proteinase K-treated preparations of A. <t>pleuropneumoniae</t> reference strain S4074 (lane 1) and strain FMV 91-6514 (lane 2). The immunoblot was probed with a monoclonal antibody against A. pleuropneumoniae serotype 1 O-antigen. Molecular mass markers (in kilodaltons) are indicated on the left.
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Image Search Results


Immunoblot of whole-cell, proteinase K-treated preparations of A. pleuropneumoniae reference strain S4074 (lane 1) and strain FMV 91-6514 (lane 2). The immunoblot was probed with a monoclonal antibody against A. pleuropneumoniae serotype 1 O-antigen. Molecular mass markers (in kilodaltons) are indicated on the left.

Journal:

Article Title: Isolation of an Atypical Strain of Actinobacillus pleuropneumoniae Serotype 1 with a Truncated Lipopolysaccharide Outer Core and No O-Antigen

doi: 10.1128/JCM.43.7.3522-3525.2005

Figure Lengend Snippet: Immunoblot of whole-cell, proteinase K-treated preparations of A. pleuropneumoniae reference strain S4074 (lane 1) and strain FMV 91-6514 (lane 2). The immunoblot was probed with a monoclonal antibody against A. pleuropneumoniae serotype 1 O-antigen. Molecular mass markers (in kilodaltons) are indicated on the left.

Article Snippet: A. pleuropneumoniae field isolate FMV 91-6514 and reference strain S4074 (ATCC 27088) were grown on brain heart infusion agar plates (Difco Laboratories, Detroit, MI) supplemented with 15 μg/ml of NAD.

Techniques: Western Blot

Silver-stained Tricine-SDS-PAGE profiles of whole-cell, proteinase K-treated preparations of A. pleuropneumoniae reference strain S4074 (lane 2), strain FMV 91-6514 (lane 3), and strain FMV 91-6514 recovered from lungs of experimentally infected animals (lane 4). For comparison, LPS from Salmonella enterica serovar Typhimurium TV119 (Ra mutant, lane 1) and SL1181 (Re mutant, lane 5) are shown. Molecular mass markers (in kilodaltons) are indicated on the left.

Journal:

Article Title: Isolation of an Atypical Strain of Actinobacillus pleuropneumoniae Serotype 1 with a Truncated Lipopolysaccharide Outer Core and No O-Antigen

doi: 10.1128/JCM.43.7.3522-3525.2005

Figure Lengend Snippet: Silver-stained Tricine-SDS-PAGE profiles of whole-cell, proteinase K-treated preparations of A. pleuropneumoniae reference strain S4074 (lane 2), strain FMV 91-6514 (lane 3), and strain FMV 91-6514 recovered from lungs of experimentally infected animals (lane 4). For comparison, LPS from Salmonella enterica serovar Typhimurium TV119 (Ra mutant, lane 1) and SL1181 (Re mutant, lane 5) are shown. Molecular mass markers (in kilodaltons) are indicated on the left.

Article Snippet: A. pleuropneumoniae field isolate FMV 91-6514 and reference strain S4074 (ATCC 27088) were grown on brain heart infusion agar plates (Difco Laboratories, Detroit, MI) supplemented with 15 μg/ml of NAD.

Techniques: Staining, SDS Page, Infection, Mutagenesis

Primers used for PCR amplification of  A. pleuropneumoniae  serotype 1 genes involved in LPS biosynthesis

Journal:

Article Title: Isolation of an Atypical Strain of Actinobacillus pleuropneumoniae Serotype 1 with a Truncated Lipopolysaccharide Outer Core and No O-Antigen

doi: 10.1128/JCM.43.7.3522-3525.2005

Figure Lengend Snippet: Primers used for PCR amplification of A. pleuropneumoniae serotype 1 genes involved in LPS biosynthesis

Article Snippet: A. pleuropneumoniae field isolate FMV 91-6514 and reference strain S4074 (ATCC 27088) were grown on brain heart infusion agar plates (Difco Laboratories, Detroit, MI) supplemented with 15 μg/ml of NAD.

Techniques: Amplification, Sequencing

Negative ion capillary electrophoresis-electrospray-MS data and proposed compositions of O-deacylated LPS from  A. pleuropneumoniae  strain FMV 91-6514 a

Journal:

Article Title: Isolation of an Atypical Strain of Actinobacillus pleuropneumoniae Serotype 1 with a Truncated Lipopolysaccharide Outer Core and No O-Antigen

doi: 10.1128/JCM.43.7.3522-3525.2005

Figure Lengend Snippet: Negative ion capillary electrophoresis-electrospray-MS data and proposed compositions of O-deacylated LPS from A. pleuropneumoniae strain FMV 91-6514 a

Article Snippet: A. pleuropneumoniae field isolate FMV 91-6514 and reference strain S4074 (ATCC 27088) were grown on brain heart infusion agar plates (Difco Laboratories, Detroit, MI) supplemented with 15 μg/ml of NAD.

Techniques: Electrophoresis