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  • 95
    Chem Impex International sodium fluoride
    Sodium Fluoride, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium fluoride/product/Chem Impex International
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    93
    Carl Zeiss halogen light
    Halogen Light, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Carl Zeiss stemi 305 lab microscope
    Stemi 305 Lab Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Cell Signaling Technology Inc anti synaptophysin 7h12
    <t>Synaptophysin</t> expression decreases in the hippocampal CA1 region of 18‐month TgF344‐AD rats. (A) Sagittal section of the dorsal hippocampus of an 18‐month wild‐type (WT) rat and (B) 18‐month TgF344‐AD (TG) rat immunofluorescently labelled using the cell nucleus marker DAPI (magenta), the amyloid plaque marker Aβ 1‐42 (white), and the presynaptic marker synaptophysin (Syp; green). Scale bar = 500 µm. (C) There were no significant differences in synaptophysin expression between WT and TG rats at 6 and 12 months of age. However, 18‐month TG rats presented with lower levels of synaptophysin staining at 18‐months compared to age‐matched WT controls. Conversely, there were no significant changes in synaptophysin expression with age or amyloid plaque accumulation in the (D) CA3 and (E) dentate gyrus (DG) regions. Data are presented as the mean ± 95% confidence intervals and analysed using a two‐way ANOVA with Holm‐Sidak post hoc test. * represents a P value < 0.05. The number (n) of rats per group was as follows: 6m WT ( n = 4); 12m WT ( n = 5); 18m WT ( n = 8); 6m TG ( n = 8); 12m TG ( n = 6); 18m TG ( n = 5).
    Anti Synaptophysin 7h12, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Carl Zeiss image browser r3 0 database
    <t>Synaptophysin</t> expression decreases in the hippocampal CA1 region of 18‐month TgF344‐AD rats. (A) Sagittal section of the dorsal hippocampus of an 18‐month wild‐type (WT) rat and (B) 18‐month TgF344‐AD (TG) rat immunofluorescently labelled using the cell nucleus marker DAPI (magenta), the amyloid plaque marker Aβ 1‐42 (white), and the presynaptic marker synaptophysin (Syp; green). Scale bar = 500 µm. (C) There were no significant differences in synaptophysin expression between WT and TG rats at 6 and 12 months of age. However, 18‐month TG rats presented with lower levels of synaptophysin staining at 18‐months compared to age‐matched WT controls. Conversely, there were no significant changes in synaptophysin expression with age or amyloid plaque accumulation in the (D) CA3 and (E) dentate gyrus (DG) regions. Data are presented as the mean ± 95% confidence intervals and analysed using a two‐way ANOVA with Holm‐Sidak post hoc test. * represents a P value < 0.05. The number (n) of rats per group was as follows: 6m WT ( n = 4); 12m WT ( n = 5); 18m WT ( n = 8); 6m TG ( n = 8); 12m TG ( n = 6); 18m TG ( n = 5).
    Image Browser R3 0 Database, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Synaptophysin expression decreases in the hippocampal CA1 region of 18‐month TgF344‐AD rats. (A) Sagittal section of the dorsal hippocampus of an 18‐month wild‐type (WT) rat and (B) 18‐month TgF344‐AD (TG) rat immunofluorescently labelled using the cell nucleus marker DAPI (magenta), the amyloid plaque marker Aβ 1‐42 (white), and the presynaptic marker synaptophysin (Syp; green). Scale bar = 500 µm. (C) There were no significant differences in synaptophysin expression between WT and TG rats at 6 and 12 months of age. However, 18‐month TG rats presented with lower levels of synaptophysin staining at 18‐months compared to age‐matched WT controls. Conversely, there were no significant changes in synaptophysin expression with age or amyloid plaque accumulation in the (D) CA3 and (E) dentate gyrus (DG) regions. Data are presented as the mean ± 95% confidence intervals and analysed using a two‐way ANOVA with Holm‐Sidak post hoc test. * represents a P value < 0.05. The number (n) of rats per group was as follows: 6m WT ( n = 4); 12m WT ( n = 5); 18m WT ( n = 8); 6m TG ( n = 8); 12m TG ( n = 6); 18m TG ( n = 5).

    Journal: FEBS Open Bio

    Article Title: Spatiotemporal immunolocalisation of REST in the brain of healthy ageing and Alzheimer’s disease rats

    doi: 10.1002/2211-5463.13036

    Figure Lengend Snippet: Synaptophysin expression decreases in the hippocampal CA1 region of 18‐month TgF344‐AD rats. (A) Sagittal section of the dorsal hippocampus of an 18‐month wild‐type (WT) rat and (B) 18‐month TgF344‐AD (TG) rat immunofluorescently labelled using the cell nucleus marker DAPI (magenta), the amyloid plaque marker Aβ 1‐42 (white), and the presynaptic marker synaptophysin (Syp; green). Scale bar = 500 µm. (C) There were no significant differences in synaptophysin expression between WT and TG rats at 6 and 12 months of age. However, 18‐month TG rats presented with lower levels of synaptophysin staining at 18‐months compared to age‐matched WT controls. Conversely, there were no significant changes in synaptophysin expression with age or amyloid plaque accumulation in the (D) CA3 and (E) dentate gyrus (DG) regions. Data are presented as the mean ± 95% confidence intervals and analysed using a two‐way ANOVA with Holm‐Sidak post hoc test. * represents a P value < 0.05. The number (n) of rats per group was as follows: 6m WT ( n = 4); 12m WT ( n = 5); 18m WT ( n = 8); 6m TG ( n = 8); 12m TG ( n = 6); 18m TG ( n = 5).

    Article Snippet: Sections from WT Fischer‐344 and TgF344‐AD rats were incubated overnight at 22 ± 2 °C with the following primary antibodies, (1) 1 : 1000 dilution of rabbit anti‐amyloid β 1–42 (mOC98) (ab201061, RRID: AB_2722492, Abcam, Cambridge, UK), a conformation‐specific antibody that recognises a discontinuous epitope of Aβ that maps to segments AEFRHD and EDVGSNK; (2) 1 : 1000 dilution of chicken antiglial fibrillary acidic protein (GFAP) (ab4674, RRID: AB_304558, Abcam); (3) 1 : 500 dilution of rabbit anti‐REST (ab202962, Abcam); (4) 1 : 5000 dilution of chicken antineurofilament heavy chain (NFH) (ab5539, RRID: AB_11212161, Merck Millipore, Watford, UK); (5) 1 : 2000 dilution of mouse anti‐synaptophysin (7H12) (9020S, RRID: AB_2631095, Cell Signalling, Leiden, The Netherlands).

    Techniques: Expressing, Marker, Staining