Journal: Molecules

Article Title: Solid-Phase Synthesis of an Insect Pyrokinin Analog Incorporating an Imidazoline Ring as Isosteric Replacement of a trans Peptide Bond

doi: 10.3390/molecules26113271

Figure Lengend Snippet: Synthesis of the iminoether 2 derived from Fmoc-L-Ala-NH 2 1 , followed by ring formation on solid support: (i) 1.1 eq. triethyloxonium tetrafluoroborate or triethyloxonium hexafluorophosphate in DCM, rt, 2 h; (ii) washing with 1 M KHCO 3 aq., overnight drying over anhydrous MgSO 4 ; (iii) DIEA/DCM, rt, overnight; (iv) Boc 2 O/DIEA, rt, 3 h.

Article Snippet: Fmoc-Ala-OSu, Fmoc-OSu, H-Ala-NH 2 , Rink resin, HBTU, HOBt, DIEA, and Fmoc-Dap(Fmoc)-OH were purchased from ChemImpex, Inc. (Wood Dale, IL, USA) or IRIS Biotech (Marktredwitz, Germany).

Techniques: Derivative Assay

Journal: Cell reports

Article Title: Selective Killing of RAS-Malignant Tissues by Exploiting Oncogene-Induced DNA Damage.

doi: 10.1016/j.celrep.2019.06.004

Figure Lengend Snippet: Figure 6. Selective Killing of RasV12-Malignant Tissues by Chemical Therapy (A, B, D, and F) Late third-instar wing discs expressing the indicated transgenes under the control of the hh-gal4 driver (which is expressed in posterior [p] cells) and stained for p53-sensor (GFP or white, A), pERK (red or white, B), Dcp1 (red or white, D and F), Ci (red in A, green in D and F, to mark the anterior [a] compartment), and DAPI (blue). In (D) and (F), larvae were subjected to different doses of IR. In (F), larvae were fed 1 mM trametinib (TRA). The p compartment is marked by a yellow line. Scale bar, 50 mm; all images are taken at the same magnification. (C) Cartoon depicting the temporal induction of transgene expression and chemical treatment. (E and G) Scatterplots showing the amount of cell death, measured as the percentage of the area covered by Dcp1-positive cells, of wing discs expressing the indicated transgenes under the control of the hh-gal4 driver and subjected to the indicated doses of IR. Average, SD, and individual wing discs measurements are shown. Statistically significant differences on the basis of Student’s t test are indicated: **p < 0.01 and ***p < 0.001; ns, not significant. Comparisons of differences (in blue) were performed to quantify the differences in the radiosensitivity of RasV12, scrib-i versus wild-type tissues upon ERK depletion (E) or TRA treatment (G) and t test (in red) to quantify the greatest differences in the amount of apoptosis between the different genotypes. Samples were dissected from late third-instar larvae raised at 29C for 3 days.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-dMMP1 (14A3D2) Developmental Studies Hybridoma bank RRID:AB_579782 rat anti-E-cadherin (1:50) Developmental Studies Hybridoma bank RRID:AB_528120 mouse anti-P-H2Av (1:500); Developmental Studies Hybridoma bank RRID:AB_2618077 rat anti-Ci Developmental Studies Hybridoma bank RRID:AB_2109711 rat anti-Elav Developmental Studies Hybridoma bank RRID:AB_528218 rabbit anti-CycE Santa Cruz Biotechnology RRID:AB_2275345 rabbit anti-Gal4 Santa Cruz Biotechnology sc-577 discontinued rabbit polyclonal anti-p-ERK (4370) Cell Signaling Technology RRID:AB_2315112 rabbit anti-cleaved Dcp1 Cell Signaling Technology RRID:AB_2721060 rabbit anti-pAkt Cell Signaling Technology RRID:AB_331414 rabbit anti-PH3 Millipore Code: 06-570, RRID:AB_310177 rabbit anti-Capicua Forés et al., 2017 N/A rabbit anti-P-H2Av (S137); Rockland Code: 600-401-914, RRID:AB_828383 Alexa fluor 488-conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) Jackson ImmunoResearch Code: 715-545-151, RRID:AB_2341099 Cy2 AffiniPure Donkey Anti-Rabbit IgG (H+L) Jackson ImmunoResearch Code: 711-225-152, RRID:AB_2340612 Cy3 AffiniPure Donkey Anti-Mouse IgG (H+L) Jackson ImmunoResearch Code: 715-165-151, RRID:AB_2315777 Cy3 AffiniPure Donkey Anti-Rabbit IgG (H+L) Jackson ImmunoResearch Code: 711-165-152, RRID:AB_2307443 Cy5 AffiniPure Donkey Anti-Rat IgG (H+L) Jackson ImmunoResearch Code: 712-175-153, RRID:AB_2340672 Chemicals, Peptides and Recombinant Proteins In Situ Cell Death Detection Kit, Fluorescein (TUNEL) Roche Code:11684 795910 Click-iT Plus EdU Alexa Fluor 647 Imaging Kit Invitrogen Code: C10640 Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit Invitrogen Code: C10632 Comet Assay Kit Trevigen Code: 4250-050K DAPI Sigma Aldrich Code: 28718-90-3 Trametinib MedChem Express Code: HY-10999A CellEventTM Senescence Green detection kit Invitrogen Code: C10850 Experimental Models.

Techniques: Expressing, Control, Staining