8317 Search Results


wild type shp2 expressing plasmid pj3  (Addgene inc)
86
Addgene inc wild type shp2 expressing plasmid pj3
<t>SHP2</t> inhibits CRC cell proliferation and migration. ( A ) SHP2 knockdown by siSHP2#1 and #2 markedly increased the proliferation of HCT116 and SW480 cells. ( B ) Colony formation assays were conducted to estimate the growth rate of HCT116 and SW480 cells. SHP2 knockdown increased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( C ) Transwell assay was performed to access the effect of SHP2 on cell migration by siRNA mediated knockdown. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. ( D ) SW480 cells were transfected with pJ-SHP2 plasmid to overexpress SHP2 and the cell proliferation was accessed by MTT assay. ( E ) Colony formation assay was done by overexpression of SHP2 in SW480 cells. SHP2 overexpression decreased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( F ) Transwell assay was performed to access the effect of SHP2 on cell migration by overexpressing SHP2 in SW480 cells. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. Overexpression of SHP2 in HCT116 and SW480 cells rescued SHP2 knockdown induced cell proliferation ( G ), colony formation ( H ) and cell migration ( I ). ( J and K ) PHPS1 improved HCT116 and SW480 cell proliferation during 3 days in a time- and dose-dependent manner. ( L ) Cells were treated with PHPS1, and colony formation was measured after two weeks by crystal violet staining. PHPS1 increased the number of CRC cell colonies. (M)Transwell assay showing blockade of SHP2 phosphatase activity by PHPS1 improved CRC cell migratory ability. NC, non-silencing control siRNA. Values represent mean ± SEM (n = 3–4), *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
Wild Type Shp2 Expressing Plasmid Pj3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8317/pmc05730547-156-0-13?v=Addgene+inc
Average 86 stars, based on 1 article reviews
wild type shp2 expressing plasmid pj3 - by Bioz Stars, 2026-08
86/100 stars
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anoxybacillus  (ATCC)
90
ATCC anoxybacillus
<t>SHP2</t> inhibits CRC cell proliferation and migration. ( A ) SHP2 knockdown by siSHP2#1 and #2 markedly increased the proliferation of HCT116 and SW480 cells. ( B ) Colony formation assays were conducted to estimate the growth rate of HCT116 and SW480 cells. SHP2 knockdown increased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( C ) Transwell assay was performed to access the effect of SHP2 on cell migration by siRNA mediated knockdown. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. ( D ) SW480 cells were transfected with pJ-SHP2 plasmid to overexpress SHP2 and the cell proliferation was accessed by MTT assay. ( E ) Colony formation assay was done by overexpression of SHP2 in SW480 cells. SHP2 overexpression decreased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( F ) Transwell assay was performed to access the effect of SHP2 on cell migration by overexpressing SHP2 in SW480 cells. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. Overexpression of SHP2 in HCT116 and SW480 cells rescued SHP2 knockdown induced cell proliferation ( G ), colony formation ( H ) and cell migration ( I ). ( J and K ) PHPS1 improved HCT116 and SW480 cell proliferation during 3 days in a time- and dose-dependent manner. ( L ) Cells were treated with PHPS1, and colony formation was measured after two weeks by crystal violet staining. PHPS1 increased the number of CRC cell colonies. (M)Transwell assay showing blockade of SHP2 phosphatase activity by PHPS1 improved CRC cell migratory ability. NC, non-silencing control siRNA. Values represent mean ± SEM (n = 3–4), *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
Anoxybacillus, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8317/10__5897_slash_ajmr2019__9066-253-241-270?v=ATCC
Average 90 stars, based on 1 article reviews
anoxybacillus - by Bioz Stars, 2026-08
90/100 stars
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znf451 3 lazsul antibodies  (Biosynth Carbosynth)
93
Biosynth Carbosynth znf451 3 lazsul antibodies
<t>SHP2</t> inhibits CRC cell proliferation and migration. ( A ) SHP2 knockdown by siSHP2#1 and #2 markedly increased the proliferation of HCT116 and SW480 cells. ( B ) Colony formation assays were conducted to estimate the growth rate of HCT116 and SW480 cells. SHP2 knockdown increased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( C ) Transwell assay was performed to access the effect of SHP2 on cell migration by siRNA mediated knockdown. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. ( D ) SW480 cells were transfected with pJ-SHP2 plasmid to overexpress SHP2 and the cell proliferation was accessed by MTT assay. ( E ) Colony formation assay was done by overexpression of SHP2 in SW480 cells. SHP2 overexpression decreased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( F ) Transwell assay was performed to access the effect of SHP2 on cell migration by overexpressing SHP2 in SW480 cells. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. Overexpression of SHP2 in HCT116 and SW480 cells rescued SHP2 knockdown induced cell proliferation ( G ), colony formation ( H ) and cell migration ( I ). ( J and K ) PHPS1 improved HCT116 and SW480 cell proliferation during 3 days in a time- and dose-dependent manner. ( L ) Cells were treated with PHPS1, and colony formation was measured after two weeks by crystal violet staining. PHPS1 increased the number of CRC cell colonies. (M)Transwell assay showing blockade of SHP2 phosphatase activity by PHPS1 improved CRC cell migratory ability. NC, non-silencing control siRNA. Values represent mean ± SEM (n = 3–4), *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
Znf451 3 Lazsul Antibodies, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8317/pm37531430-243-2-18?v=Biosynth+Carbosynth
Average 93 stars, based on 1 article reviews
znf451 3 lazsul antibodies - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
3s  (Chem Impex International)
96
Chem Impex International 3s
<t>SHP2</t> inhibits CRC cell proliferation and migration. ( A ) SHP2 knockdown by siSHP2#1 and #2 markedly increased the proliferation of HCT116 and SW480 cells. ( B ) Colony formation assays were conducted to estimate the growth rate of HCT116 and SW480 cells. SHP2 knockdown increased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( C ) Transwell assay was performed to access the effect of SHP2 on cell migration by siRNA mediated knockdown. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. ( D ) SW480 cells were transfected with pJ-SHP2 plasmid to overexpress SHP2 and the cell proliferation was accessed by MTT assay. ( E ) Colony formation assay was done by overexpression of SHP2 in SW480 cells. SHP2 overexpression decreased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( F ) Transwell assay was performed to access the effect of SHP2 on cell migration by overexpressing SHP2 in SW480 cells. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. Overexpression of SHP2 in HCT116 and SW480 cells rescued SHP2 knockdown induced cell proliferation ( G ), colony formation ( H ) and cell migration ( I ). ( J and K ) PHPS1 improved HCT116 and SW480 cell proliferation during 3 days in a time- and dose-dependent manner. ( L ) Cells were treated with PHPS1, and colony formation was measured after two weeks by crystal violet staining. PHPS1 increased the number of CRC cell colonies. (M)Transwell assay showing blockade of SHP2 phosphatase activity by PHPS1 improved CRC cell migratory ability. NC, non-silencing control siRNA. Values represent mean ± SEM (n = 3–4), *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
3s, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8317/us08716303-1302-3-5?v=Chem+Impex+International
Average 96 stars, based on 1 article reviews
3s - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
kng2 rabbit antibodybcn 8317  (AntibodyBcn)
90
AntibodyBcn kng2 rabbit antibodybcn 8317
<t>SHP2</t> inhibits CRC cell proliferation and migration. ( A ) SHP2 knockdown by siSHP2#1 and #2 markedly increased the proliferation of HCT116 and SW480 cells. ( B ) Colony formation assays were conducted to estimate the growth rate of HCT116 and SW480 cells. SHP2 knockdown increased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( C ) Transwell assay was performed to access the effect of SHP2 on cell migration by siRNA mediated knockdown. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. ( D ) SW480 cells were transfected with pJ-SHP2 plasmid to overexpress SHP2 and the cell proliferation was accessed by MTT assay. ( E ) Colony formation assay was done by overexpression of SHP2 in SW480 cells. SHP2 overexpression decreased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( F ) Transwell assay was performed to access the effect of SHP2 on cell migration by overexpressing SHP2 in SW480 cells. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. Overexpression of SHP2 in HCT116 and SW480 cells rescued SHP2 knockdown induced cell proliferation ( G ), colony formation ( H ) and cell migration ( I ). ( J and K ) PHPS1 improved HCT116 and SW480 cell proliferation during 3 days in a time- and dose-dependent manner. ( L ) Cells were treated with PHPS1, and colony formation was measured after two weeks by crystal violet staining. PHPS1 increased the number of CRC cell colonies. (M)Transwell assay showing blockade of SHP2 phosphatase activity by PHPS1 improved CRC cell migratory ability. NC, non-silencing control siRNA. Values represent mean ± SEM (n = 3–4), *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
Kng2 Rabbit Antibodybcn 8317, supplied by AntibodyBcn, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8317/pmc07195474__41467_2020_16009_MOESM3_ESM-31-13-16?v=AntibodyBcn
Average 90 stars, based on 1 article reviews
kng2 rabbit antibodybcn 8317 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


SHP2 inhibits CRC cell proliferation and migration. ( A ) SHP2 knockdown by siSHP2#1 and #2 markedly increased the proliferation of HCT116 and SW480 cells. ( B ) Colony formation assays were conducted to estimate the growth rate of HCT116 and SW480 cells. SHP2 knockdown increased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( C ) Transwell assay was performed to access the effect of SHP2 on cell migration by siRNA mediated knockdown. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. ( D ) SW480 cells were transfected with pJ-SHP2 plasmid to overexpress SHP2 and the cell proliferation was accessed by MTT assay. ( E ) Colony formation assay was done by overexpression of SHP2 in SW480 cells. SHP2 overexpression decreased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( F ) Transwell assay was performed to access the effect of SHP2 on cell migration by overexpressing SHP2 in SW480 cells. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. Overexpression of SHP2 in HCT116 and SW480 cells rescued SHP2 knockdown induced cell proliferation ( G ), colony formation ( H ) and cell migration ( I ). ( J and K ) PHPS1 improved HCT116 and SW480 cell proliferation during 3 days in a time- and dose-dependent manner. ( L ) Cells were treated with PHPS1, and colony formation was measured after two weeks by crystal violet staining. PHPS1 increased the number of CRC cell colonies. (M)Transwell assay showing blockade of SHP2 phosphatase activity by PHPS1 improved CRC cell migratory ability. NC, non-silencing control siRNA. Values represent mean ± SEM (n = 3–4), *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.

Journal: Scientific Reports

Article Title: SHP2 associates with nuclear localization of STAT3: significance in progression and prognosis of colorectal cancer

doi: 10.1038/s41598-017-17604-7

Figure Lengend Snippet: SHP2 inhibits CRC cell proliferation and migration. ( A ) SHP2 knockdown by siSHP2#1 and #2 markedly increased the proliferation of HCT116 and SW480 cells. ( B ) Colony formation assays were conducted to estimate the growth rate of HCT116 and SW480 cells. SHP2 knockdown increased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( C ) Transwell assay was performed to access the effect of SHP2 on cell migration by siRNA mediated knockdown. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. ( D ) SW480 cells were transfected with pJ-SHP2 plasmid to overexpress SHP2 and the cell proliferation was accessed by MTT assay. ( E ) Colony formation assay was done by overexpression of SHP2 in SW480 cells. SHP2 overexpression decreased the colony numbers compared with the control group. Representative pictures of colonies (left) and quantification of colony numbers (right) are shown. ( F ) Transwell assay was performed to access the effect of SHP2 on cell migration by overexpressing SHP2 in SW480 cells. Representative pictures of cells (left) and quantification of cell numbers (right) are shown. Overexpression of SHP2 in HCT116 and SW480 cells rescued SHP2 knockdown induced cell proliferation ( G ), colony formation ( H ) and cell migration ( I ). ( J and K ) PHPS1 improved HCT116 and SW480 cell proliferation during 3 days in a time- and dose-dependent manner. ( L ) Cells were treated with PHPS1, and colony formation was measured after two weeks by crystal violet staining. PHPS1 increased the number of CRC cell colonies. (M)Transwell assay showing blockade of SHP2 phosphatase activity by PHPS1 improved CRC cell migratory ability. NC, non-silencing control siRNA. Values represent mean ± SEM (n = 3–4), *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.

Article Snippet: Wild-type SHP2 expressing plasmid pJ3 (pJ3-SHP2 WT) was a gift from Ben Neel (Addgene plasmid # 8317) .

Techniques: Migration, Knockdown, Control, Transwell Assay, Transfection, Plasmid Preparation, MTT Assay, Colony Assay, Over Expression, Staining, Activity Assay

SHP2 suppressing role in CRC is mediated by STAT3 dephosphorylation. ( A ) STAT3 phosphorylation was increased in CRC cells knocked down for SHP2 by siRNA#1 and #2. ( B ) SHP2 overexpression reduced STAT3 phosphorylation in SW480. ( C ) PHPS1 enhanced the levels of pSTAT3 in 10 mg/L time-dependently. ( D ) Nuclear distribution of pSTAT3 was enhanced after SHP2 knockdown (×400). ( E ) Cryptotanshinone significantly reversed CRC cell proliferation induced by PHPS1. Values represent mean ± SEM (n = 3), **P ≤ 0.01, compared with Vehicle group. ( F ) SW480 was more sensitive to IL-6-induced proliferation after SHP2 knockdown, which was rescued by SHP2 overexpression; meanwhile overexpression SHP2 inhibited IL-6 induced SW480 proliferation. Values represent mean ± SEM (n = 3) # P ≤ 0.05, **P ≤ 0.01, compared with NC + pJ3 group. Whole blots are shown in Supplementary Fig. .

Journal: Scientific Reports

Article Title: SHP2 associates with nuclear localization of STAT3: significance in progression and prognosis of colorectal cancer

doi: 10.1038/s41598-017-17604-7

Figure Lengend Snippet: SHP2 suppressing role in CRC is mediated by STAT3 dephosphorylation. ( A ) STAT3 phosphorylation was increased in CRC cells knocked down for SHP2 by siRNA#1 and #2. ( B ) SHP2 overexpression reduced STAT3 phosphorylation in SW480. ( C ) PHPS1 enhanced the levels of pSTAT3 in 10 mg/L time-dependently. ( D ) Nuclear distribution of pSTAT3 was enhanced after SHP2 knockdown (×400). ( E ) Cryptotanshinone significantly reversed CRC cell proliferation induced by PHPS1. Values represent mean ± SEM (n = 3), **P ≤ 0.01, compared with Vehicle group. ( F ) SW480 was more sensitive to IL-6-induced proliferation after SHP2 knockdown, which was rescued by SHP2 overexpression; meanwhile overexpression SHP2 inhibited IL-6 induced SW480 proliferation. Values represent mean ± SEM (n = 3) # P ≤ 0.05, **P ≤ 0.01, compared with NC + pJ3 group. Whole blots are shown in Supplementary Fig. .

Article Snippet: Wild-type SHP2 expressing plasmid pJ3 (pJ3-SHP2 WT) was a gift from Ben Neel (Addgene plasmid # 8317) .

Techniques: De-Phosphorylation Assay, Phospho-proteomics, Over Expression, Knockdown

Association between the combination of SHP2/nuclear STAT3 and survival in patients with CRC. ( A ) Kaplan–Meier analysis showed that Patients with high SHP2 and low nuclear STAT3 present better DSS (left panel) and DFS (right panel) than patients with low SHP2 and high nuclear STAT3. ( B ) Kaplan-Meier analysis showed that Patients with high SHP2 and low nuclear STAT3 present better DSS (left panel) and DFS (right panel) than patients with low SHP2 or high nuclear STAT3. ( C ) Kaplan-Meier analysis showed that patients with high SHP2 or low nuclear STAT3 present better DFS (right panel) but not DSS (left panel) than patients with low SHP2 and high nuclear STAT3. P values were determined using log-rank test.

Journal: Scientific Reports

Article Title: SHP2 associates with nuclear localization of STAT3: significance in progression and prognosis of colorectal cancer

doi: 10.1038/s41598-017-17604-7

Figure Lengend Snippet: Association between the combination of SHP2/nuclear STAT3 and survival in patients with CRC. ( A ) Kaplan–Meier analysis showed that Patients with high SHP2 and low nuclear STAT3 present better DSS (left panel) and DFS (right panel) than patients with low SHP2 and high nuclear STAT3. ( B ) Kaplan-Meier analysis showed that Patients with high SHP2 and low nuclear STAT3 present better DSS (left panel) and DFS (right panel) than patients with low SHP2 or high nuclear STAT3. ( C ) Kaplan-Meier analysis showed that patients with high SHP2 or low nuclear STAT3 present better DFS (right panel) but not DSS (left panel) than patients with low SHP2 and high nuclear STAT3. P values were determined using log-rank test.

Article Snippet: Wild-type SHP2 expressing plasmid pJ3 (pJ3-SHP2 WT) was a gift from Ben Neel (Addgene plasmid # 8317) .

Techniques:

Univariate Cox regression analysis for clinical parameters and the combinations of SHP2 and nuclear STAT3.

Journal: Scientific Reports

Article Title: SHP2 associates with nuclear localization of STAT3: significance in progression and prognosis of colorectal cancer

doi: 10.1038/s41598-017-17604-7

Figure Lengend Snippet: Univariate Cox regression analysis for clinical parameters and the combinations of SHP2 and nuclear STAT3.

Article Snippet: Wild-type SHP2 expressing plasmid pJ3 (pJ3-SHP2 WT) was a gift from Ben Neel (Addgene plasmid # 8317) .

Techniques: Adjuvant

Multivariate Cox regression analysis for clinical parameters and the combinations of high SHP2 and low nuclear STAT3.

Journal: Scientific Reports

Article Title: SHP2 associates with nuclear localization of STAT3: significance in progression and prognosis of colorectal cancer

doi: 10.1038/s41598-017-17604-7

Figure Lengend Snippet: Multivariate Cox regression analysis for clinical parameters and the combinations of high SHP2 and low nuclear STAT3.

Article Snippet: Wild-type SHP2 expressing plasmid pJ3 (pJ3-SHP2 WT) was a gift from Ben Neel (Addgene plasmid # 8317) .

Techniques: Adjuvant

Multivariate Cox regression analysis of DFS for clinical parameters and the combinations of low SHP2 and high nuclear STAT3.

Journal: Scientific Reports

Article Title: SHP2 associates with nuclear localization of STAT3: significance in progression and prognosis of colorectal cancer

doi: 10.1038/s41598-017-17604-7

Figure Lengend Snippet: Multivariate Cox regression analysis of DFS for clinical parameters and the combinations of low SHP2 and high nuclear STAT3.

Article Snippet: Wild-type SHP2 expressing plasmid pJ3 (pJ3-SHP2 WT) was a gift from Ben Neel (Addgene plasmid # 8317) .

Techniques: Adjuvant