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Image Search Results
Journal: PLoS ONE
Article Title: Defects in Innate Immunity Render Breast Cancer Initiating Cells Permissive to Oncolytic Adenovirus
doi: 10.1371/journal.pone.0013859
Figure Lengend Snippet: SOCS1, SOCS3 and TAM (Axl, Mer and Tyro3) receptor expression were assayed by Western blot at different time points following infection in non-CIC ArLa cells and JIMT-1 CIC and non-CIC populations. The ArLa non-CIC cells show upregulation of SOCS1 at 2 h and constant expression of SOCS3 with minor upregulation in response to adenovirus infection at 2 h (A). In contrast there is constant expression of SOCS1 in both JIMT-1 CD44 + /CD24 −/low CIC infected and non-infected and infected CD44 − /CD24 − non-CIC populations at all time points (B) and non or minor expression of SOCS3 in both populations (B). No Axl, Mer or Tyro3 induction is seen in any time point in ArLa non-CIC (C). Interestingly, JIMT-1 CD44 + /CD24 −/low CIC show constant upregulation of Axl, Mer and Tyro3 (D) in all time points and also non-infected cells. Infected CD24 + non-CIC population show constant Mer and variable Tyro3 expression but no Axl expression (D). Increased inhibitory signaling is likely to contribute to dysfunction in innate immune responses in the CIC population.
Article Snippet: Primary antibodies: STAT1, phospho-STAT1, STAT3, phospho-STAT3 and SOCS3 (Cell Signaling Technology), GAPDH (Zymed), SOCS1 (Millipore), Axl (Abcam), MerTK (Novus Biologicals),
Techniques: Expressing, Western Blot, Infection