81151 Search Results


mouse anti pgrn antibody  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology mouse anti pgrn antibody
(A, B) Expression levels of <t>PGRN</t> ( A ) <t>and</t> <t>CDK4</t> ( B ) in both gastric cancer and adjacent normal tissues as measured by immunohistochemistry. The results represent the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, paired t -test. All data are mean values of three biological replicates.
Mouse Anti Pgrn Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti pgrn antibody/product/Santa Cruz Biotechnology
Average 91 stars, based on 1 article reviews
mouse anti pgrn antibody - by Bioz Stars, 2026-02
91/100 stars
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rhodamine ubiquitin  (BPS Bioscience)
91
BPS Bioscience rhodamine ubiquitin
PALB2WD40 directly binds USP22 and stimulates its catalytic DUB activity. a. In-silico model using ZDOCK of USP22DUB binding PALB2WD40 predicting key residues involved in the interaction. b. U2OSFOKI cells co-transfected with the indicated mCherry-LacI protein to tether it to 256X LacO array along with GFP-USP22. Percentage of cells with recruitment of GFP-USP22 to LacO array are indicated with +/− SD. White scale bars indicated 5um. 120 cells were counted for each condition for every experimental round. Experiment was done in triplicate. c. Immunoprecipitation of mcherry-PALB2 fragments plasmids transiently transfected into H1299 cells. Western blot against mCherry, FLAG (FLAG-USP22), Rad51. mCherry alone is used as a negative control. Numbers to the left of western blots indicate molecular weight. d. In-vitro pulldown using recombinant MBP-PALB2WD40 (prey) and His-USP22FL (bait). MBP was also incubated with His-USP22 as a negative control. Western blot was performed against His (His-USP22) and MBP (MBP-PALB2WD40, MBP). Numbers to the left of western blots indicate molecular weight. e. <t>Ubiquitin</t> cleavage assay using rhodamine-ubiquitin that is quenched and upon cleavage of ubiquitin fluoresces. Proteins indicated below were pre-incubated to form their respective complexes then rhodamine-ubiquitin was added. Units on vertical axis indicate raw fluorescent units at 565nm. Buffer alone was used as a negative control for auto-fluorescence baseline. Error bars indicated +/− SD. Experiment was done in triplicate.
Rhodamine Ubiquitin, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rhodamine ubiquitin/product/BPS Bioscience
Average 91 stars, based on 1 article reviews
rhodamine ubiquitin - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier

Image Search Results


(A, B) Expression levels of PGRN ( A ) and CDK4 ( B ) in both gastric cancer and adjacent normal tissues as measured by immunohistochemistry. The results represent the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, paired t -test. All data are mean values of three biological replicates.

Journal: Journal of Microbiology and Biotechnology

Article Title: Helicobacter pylori -Induced Progranulin Promotes the Progression of the Gastric Epithelial Cell Cycle by Regulating CDK4

doi: 10.4014/jmb.2203.03053

Figure Lengend Snippet: (A, B) Expression levels of PGRN ( A ) and CDK4 ( B ) in both gastric cancer and adjacent normal tissues as measured by immunohistochemistry. The results represent the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, paired t -test. All data are mean values of three biological replicates.

Article Snippet: They were then incubated at 4°C overnight after adding mouse anti-PGRN antibody (1:100, sc-377036, Santa Cruz) or rabbit anti-CDK4 antibody (1:800, #12790, Cell Signaling Technology) according to the instructions.

Techniques: Expressing, Immunohistochemistry

Correlations between PGRN and CDK4 in gastric cancer and adjacent normal tissues analyzed by linear regression.

Journal: Journal of Microbiology and Biotechnology

Article Title: Helicobacter pylori -Induced Progranulin Promotes the Progression of the Gastric Epithelial Cell Cycle by Regulating CDK4

doi: 10.4014/jmb.2203.03053

Figure Lengend Snippet: Correlations between PGRN and CDK4 in gastric cancer and adjacent normal tissues analyzed by linear regression.

Article Snippet: They were then incubated at 4°C overnight after adding mouse anti-PGRN antibody (1:100, sc-377036, Santa Cruz) or rabbit anti-CDK4 antibody (1:800, #12790, Cell Signaling Technology) according to the instructions.

Techniques:

( A, B ) qPCR and western blot analysis of the expression of CDK4 in BGC-823 cells infected with H. pylori 26695 at a MOI of 100: 1. ( C, D ) qPCR and western blot analysis of the expression of CDK4 after transfection with pLKO.1-PGRN shRNA-GFP and Plenti6/V5-PGRN lentivirus. ( E, F ) CDK4 expression after transfection with CDK4-RNAi-13, CDK4-RNAi-14, CDK4-RNAi-15 of CDK4-knockdown lentivirus. ( G ) Flow cytometry analysis of the cell cycle changes of CDK4 knockdown and coculture with H. pylori in 50:1 MOI in BGC-823 cells. ( H ) Flow cytometry analysis of the cell cycle changes of CDK4 knockdown cell lines infected with PGRN-knockdown /overexpressed lentivirus and cocultured with H. pylori at a MOI of 50: 1. The results represent the mean ± SD of three independent experiments. SI, PGRN knockdown group, NS, the control group of PGRN knockdown, GFP, the control group of PGRN overexpressing, ns, not significant, HP, Helicobacter pylori , * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Journal of Microbiology and Biotechnology

Article Title: Helicobacter pylori -Induced Progranulin Promotes the Progression of the Gastric Epithelial Cell Cycle by Regulating CDK4

doi: 10.4014/jmb.2203.03053

Figure Lengend Snippet: ( A, B ) qPCR and western blot analysis of the expression of CDK4 in BGC-823 cells infected with H. pylori 26695 at a MOI of 100: 1. ( C, D ) qPCR and western blot analysis of the expression of CDK4 after transfection with pLKO.1-PGRN shRNA-GFP and Plenti6/V5-PGRN lentivirus. ( E, F ) CDK4 expression after transfection with CDK4-RNAi-13, CDK4-RNAi-14, CDK4-RNAi-15 of CDK4-knockdown lentivirus. ( G ) Flow cytometry analysis of the cell cycle changes of CDK4 knockdown and coculture with H. pylori in 50:1 MOI in BGC-823 cells. ( H ) Flow cytometry analysis of the cell cycle changes of CDK4 knockdown cell lines infected with PGRN-knockdown /overexpressed lentivirus and cocultured with H. pylori at a MOI of 50: 1. The results represent the mean ± SD of three independent experiments. SI, PGRN knockdown group, NS, the control group of PGRN knockdown, GFP, the control group of PGRN overexpressing, ns, not significant, HP, Helicobacter pylori , * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: They were then incubated at 4°C overnight after adding mouse anti-PGRN antibody (1:100, sc-377036, Santa Cruz) or rabbit anti-CDK4 antibody (1:800, #12790, Cell Signaling Technology) according to the instructions.

Techniques: Western Blot, Expressing, Infection, Transfection, shRNA, Knockdown, Flow Cytometry, Control

( A ) qPCR analysis of the expression of PGRN after transfection with pLKO.1-PGRN shRNA-GFP and Plenti6/V5-PGRN lentivirus and cocultured with H. pylori at a MOI of 100: 1. ( B ) Western blot analysis of CDK4 protein expression in cells pretreated with a PI3K signal pathway inhibitor (LY294002) for 2 h before transfection with pLKO.1-PGRN shRNA-GFP and Plenti6/V5-PGRN lentivirus. ( C ) qPCR analysis of the expression of CDK4 after transfection with pLKO.1-PGRN shRNA-GFP and Plenti6/V5-PGRN lentivirus and cocultured with H. pylori at a MOI of 100: 1. ( D ) Western blot analysis of CDK4, Akt, and p-Akt protein expression in cells transfected with pLKO.1-PGRN shRNA-GFP and Plenti6/V5-PGRN lentivirus and cocultured with H. pylori at a MOI of 100: 1. The results represent the mean ± SD of three independent experiments. SI, PGRN knockdown group, NS, the control group of PGRN knockdown, GFP, the control group of PGRN overexpressing, HP, Helicobacter pylori , * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Journal of Microbiology and Biotechnology

Article Title: Helicobacter pylori -Induced Progranulin Promotes the Progression of the Gastric Epithelial Cell Cycle by Regulating CDK4

doi: 10.4014/jmb.2203.03053

Figure Lengend Snippet: ( A ) qPCR analysis of the expression of PGRN after transfection with pLKO.1-PGRN shRNA-GFP and Plenti6/V5-PGRN lentivirus and cocultured with H. pylori at a MOI of 100: 1. ( B ) Western blot analysis of CDK4 protein expression in cells pretreated with a PI3K signal pathway inhibitor (LY294002) for 2 h before transfection with pLKO.1-PGRN shRNA-GFP and Plenti6/V5-PGRN lentivirus. ( C ) qPCR analysis of the expression of CDK4 after transfection with pLKO.1-PGRN shRNA-GFP and Plenti6/V5-PGRN lentivirus and cocultured with H. pylori at a MOI of 100: 1. ( D ) Western blot analysis of CDK4, Akt, and p-Akt protein expression in cells transfected with pLKO.1-PGRN shRNA-GFP and Plenti6/V5-PGRN lentivirus and cocultured with H. pylori at a MOI of 100: 1. The results represent the mean ± SD of three independent experiments. SI, PGRN knockdown group, NS, the control group of PGRN knockdown, GFP, the control group of PGRN overexpressing, HP, Helicobacter pylori , * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: They were then incubated at 4°C overnight after adding mouse anti-PGRN antibody (1:100, sc-377036, Santa Cruz) or rabbit anti-CDK4 antibody (1:800, #12790, Cell Signaling Technology) according to the instructions.

Techniques: Expressing, Transfection, shRNA, Western Blot, Knockdown, Control

PALB2WD40 directly binds USP22 and stimulates its catalytic DUB activity. a. In-silico model using ZDOCK of USP22DUB binding PALB2WD40 predicting key residues involved in the interaction. b. U2OSFOKI cells co-transfected with the indicated mCherry-LacI protein to tether it to 256X LacO array along with GFP-USP22. Percentage of cells with recruitment of GFP-USP22 to LacO array are indicated with +/− SD. White scale bars indicated 5um. 120 cells were counted for each condition for every experimental round. Experiment was done in triplicate. c. Immunoprecipitation of mcherry-PALB2 fragments plasmids transiently transfected into H1299 cells. Western blot against mCherry, FLAG (FLAG-USP22), Rad51. mCherry alone is used as a negative control. Numbers to the left of western blots indicate molecular weight. d. In-vitro pulldown using recombinant MBP-PALB2WD40 (prey) and His-USP22FL (bait). MBP was also incubated with His-USP22 as a negative control. Western blot was performed against His (His-USP22) and MBP (MBP-PALB2WD40, MBP). Numbers to the left of western blots indicate molecular weight. e. Ubiquitin cleavage assay using rhodamine-ubiquitin that is quenched and upon cleavage of ubiquitin fluoresces. Proteins indicated below were pre-incubated to form their respective complexes then rhodamine-ubiquitin was added. Units on vertical axis indicate raw fluorescent units at 565nm. Buffer alone was used as a negative control for auto-fluorescence baseline. Error bars indicated +/− SD. Experiment was done in triplicate.

Journal: Molecular cancer research : MCR

Article Title: USP22 interacts with PALB2 and promotes chemotherapy resistance via homologous recombination of DNA double strand breaks

doi: 10.1158/1541-7786.MCR-19-0053

Figure Lengend Snippet: PALB2WD40 directly binds USP22 and stimulates its catalytic DUB activity. a. In-silico model using ZDOCK of USP22DUB binding PALB2WD40 predicting key residues involved in the interaction. b. U2OSFOKI cells co-transfected with the indicated mCherry-LacI protein to tether it to 256X LacO array along with GFP-USP22. Percentage of cells with recruitment of GFP-USP22 to LacO array are indicated with +/− SD. White scale bars indicated 5um. 120 cells were counted for each condition for every experimental round. Experiment was done in triplicate. c. Immunoprecipitation of mcherry-PALB2 fragments plasmids transiently transfected into H1299 cells. Western blot against mCherry, FLAG (FLAG-USP22), Rad51. mCherry alone is used as a negative control. Numbers to the left of western blots indicate molecular weight. d. In-vitro pulldown using recombinant MBP-PALB2WD40 (prey) and His-USP22FL (bait). MBP was also incubated with His-USP22 as a negative control. Western blot was performed against His (His-USP22) and MBP (MBP-PALB2WD40, MBP). Numbers to the left of western blots indicate molecular weight. e. Ubiquitin cleavage assay using rhodamine-ubiquitin that is quenched and upon cleavage of ubiquitin fluoresces. Proteins indicated below were pre-incubated to form their respective complexes then rhodamine-ubiquitin was added. Units on vertical axis indicate raw fluorescent units at 565nm. Buffer alone was used as a negative control for auto-fluorescence baseline. Error bars indicated +/− SD. Experiment was done in triplicate.

Article Snippet: Rhodamine-Ubiquitin (BPS Bioscience, #81151) was then added in at a 1:1 molar ratio and incubated with the complexes at 37°C for 1 hr.

Techniques: Activity Assay, In Silico, Binding Assay, Transfection, Immunoprecipitation, Western Blot, Negative Control, Molecular Weight, In Vitro, Recombinant, Incubation, Cleavage Assay, Fluorescence