810335c Search Results


cy5 dope  (Croda International Plc)
95
Croda International Plc cy5 dope
(a) Hydropathy distribution based on Kyte-Doolible hydropathy model (pink) and net charge per residue obtained using CIDER42 (blue), both plotted as a function of the CT45 sequence, showing that it is an amphipathic and moderately positively charged peptide. The net charge for each amino acid quantifies the average electrostatic charge of a 5-residue window centered on that amino acid. (b) Schematic diagram showing the side-view of a bilayer-coated microwell array. (c) Bright-field and fluorescence images show the top views of the microwell array covered with a phospholipid bilayer. Scale bar, 20 µ m. (d) A plot showing rapid reduction in the CT45 fluorescence within the microwells upon flushing the device with PBS; the insets illustrate fluorescence images before and after washing. The lipid bilayer composition was 89.9 % DOPC + 10 % DOPS + 0.1% <t>cy5-DOPE</t> (molar ratio), and 125 µ M CT45 (CT45:TAMRA-CT45 = 10:1, molar ratio) in PBS was incubated. Data points represent mean values while the shaded areas indicate standard deviations (n = 5 microwells within one microfluidic device). Scale bar, 20 µ m. (e) Conceptual sketch illustrating that CT45 molecules do not interact with the negatively charged membrane of the liposome. (f) Fluorescence images showing the equatorial plane of a liposome (left) and the encapsulated CT45 distribution (right). Scale bar, 5 µ m. (g) Normalized fluorescence intensity (magenta: lipids; red: CT45) corresponding to the dotted line in panel (f) showing a homogeneous CT45 distribution within the lumen. (h) Conceptual sketch illustrating that CT45 molecules accumulate at the oil-water interface offered by the double emulsion confinement. (i) Bright-field (left) and fluorescence (right) images showing the equatorial plane of a DE droplet and CT45 accumulation on its inner surface. Scale bar, 20 µ m. (j) Normalized gray value (black) and fluorescence (red) intensity corresponding to the dotted lines in panel (i) show accumulation of CT45 on the inner surface of the DE confinement. In e-j, the encapsulated mixture consisted of 55 µ M CT45 (CT45:TAMRA-CT45 = 10:1, molar ratio) and 300 mM Na 2 HPO 4 . In e-g, the lipid bilayer composition was 89.9 % DOPC + 10 % DOPS + 0.1% cy5-DOPE (molar ratio). In h-j, the oil phase was fluorinated oil (HFE 7500) containing 2% FluoSurf-C surfactant.
Cy5 Dope, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/810335c/18%3A1+Cy5+PE/bio_rxiv__2025__09__10__674898-170-23-29
Average 95 stars, based on 1 article reviews
cy5 dope - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier

Image Search Results


(a) Hydropathy distribution based on Kyte-Doolible hydropathy model (pink) and net charge per residue obtained using CIDER42 (blue), both plotted as a function of the CT45 sequence, showing that it is an amphipathic and moderately positively charged peptide. The net charge for each amino acid quantifies the average electrostatic charge of a 5-residue window centered on that amino acid. (b) Schematic diagram showing the side-view of a bilayer-coated microwell array. (c) Bright-field and fluorescence images show the top views of the microwell array covered with a phospholipid bilayer. Scale bar, 20 µ m. (d) A plot showing rapid reduction in the CT45 fluorescence within the microwells upon flushing the device with PBS; the insets illustrate fluorescence images before and after washing. The lipid bilayer composition was 89.9 % DOPC + 10 % DOPS + 0.1% cy5-DOPE (molar ratio), and 125 µ M CT45 (CT45:TAMRA-CT45 = 10:1, molar ratio) in PBS was incubated. Data points represent mean values while the shaded areas indicate standard deviations (n = 5 microwells within one microfluidic device). Scale bar, 20 µ m. (e) Conceptual sketch illustrating that CT45 molecules do not interact with the negatively charged membrane of the liposome. (f) Fluorescence images showing the equatorial plane of a liposome (left) and the encapsulated CT45 distribution (right). Scale bar, 5 µ m. (g) Normalized fluorescence intensity (magenta: lipids; red: CT45) corresponding to the dotted line in panel (f) showing a homogeneous CT45 distribution within the lumen. (h) Conceptual sketch illustrating that CT45 molecules accumulate at the oil-water interface offered by the double emulsion confinement. (i) Bright-field (left) and fluorescence (right) images showing the equatorial plane of a DE droplet and CT45 accumulation on its inner surface. Scale bar, 20 µ m. (j) Normalized gray value (black) and fluorescence (red) intensity corresponding to the dotted lines in panel (i) show accumulation of CT45 on the inner surface of the DE confinement. In e-j, the encapsulated mixture consisted of 55 µ M CT45 (CT45:TAMRA-CT45 = 10:1, molar ratio) and 300 mM Na 2 HPO 4 . In e-g, the lipid bilayer composition was 89.9 % DOPC + 10 % DOPS + 0.1% cy5-DOPE (molar ratio). In h-j, the oil phase was fluorinated oil (HFE 7500) containing 2% FluoSurf-C surfactant.

Journal: bioRxiv

Article Title: Condensate-based shells and scaffolds via interfacial liquid-to-solid transition of disordered peptides

doi: 10.1101/2025.09.10.674898

Figure Lengend Snippet: (a) Hydropathy distribution based on Kyte-Doolible hydropathy model (pink) and net charge per residue obtained using CIDER42 (blue), both plotted as a function of the CT45 sequence, showing that it is an amphipathic and moderately positively charged peptide. The net charge for each amino acid quantifies the average electrostatic charge of a 5-residue window centered on that amino acid. (b) Schematic diagram showing the side-view of a bilayer-coated microwell array. (c) Bright-field and fluorescence images show the top views of the microwell array covered with a phospholipid bilayer. Scale bar, 20 µ m. (d) A plot showing rapid reduction in the CT45 fluorescence within the microwells upon flushing the device with PBS; the insets illustrate fluorescence images before and after washing. The lipid bilayer composition was 89.9 % DOPC + 10 % DOPS + 0.1% cy5-DOPE (molar ratio), and 125 µ M CT45 (CT45:TAMRA-CT45 = 10:1, molar ratio) in PBS was incubated. Data points represent mean values while the shaded areas indicate standard deviations (n = 5 microwells within one microfluidic device). Scale bar, 20 µ m. (e) Conceptual sketch illustrating that CT45 molecules do not interact with the negatively charged membrane of the liposome. (f) Fluorescence images showing the equatorial plane of a liposome (left) and the encapsulated CT45 distribution (right). Scale bar, 5 µ m. (g) Normalized fluorescence intensity (magenta: lipids; red: CT45) corresponding to the dotted line in panel (f) showing a homogeneous CT45 distribution within the lumen. (h) Conceptual sketch illustrating that CT45 molecules accumulate at the oil-water interface offered by the double emulsion confinement. (i) Bright-field (left) and fluorescence (right) images showing the equatorial plane of a DE droplet and CT45 accumulation on its inner surface. Scale bar, 20 µ m. (j) Normalized gray value (black) and fluorescence (red) intensity corresponding to the dotted lines in panel (i) show accumulation of CT45 on the inner surface of the DE confinement. In e-j, the encapsulated mixture consisted of 55 µ M CT45 (CT45:TAMRA-CT45 = 10:1, molar ratio) and 300 mM Na 2 HPO 4 . In e-g, the lipid bilayer composition was 89.9 % DOPC + 10 % DOPS + 0.1% cy5-DOPE (molar ratio). In h-j, the oil phase was fluorinated oil (HFE 7500) containing 2% FluoSurf-C surfactant.

Article Snippet: Labeled dextran (Alexa FluorTM 647, 10,000 MW, D22914) was purchased from Fischer Scientific B.V. Phospholipids including DOPC (SKU 850375C), DOPS (SKU 840035P) and cy5-DOPE (SKU 810335C) were purchased from Avanti Polar Lipids, Inc. SylgardTM 184 silicone elastomer (PDMS) and curing agent were purchased from Dow.

Techniques: Residue, Sequencing, Fluorescence, Incubation, Membrane, Double Emulsion