Journal: The ISME Journal

Article Title: Living apart together—bacterial volatiles influence methanotrophic growth and activity

doi: 10.1038/s41396-018-0055-7

Figure Lengend Snippet: Biomass (a, b) and population methane oxidation (c, d) of the methanotrophs Methylobacter luteus and Methylocystis parvus grown on two-compartment agar plates at 20% CH4 headspace, incubated alone (Control), alone with 5% CO2 (CO2 control) or in the presence of a heterotroph: Bacillus pumilus isolate YXY-10, Bacillus simplex strain DUCC3713, Stenotrophomonas maltophilia strain ATCC 13637, Exiguobacterium undae strain B111, Pseudomonas mandelii JR-1. Growth of M. luteus CO2 control was performed in a separate experiment (SI 2). Boxes represent median, first and third quartiles. Whiskers indicate the 5th and 95th percentile. Inset shows two-compartment Petri dish with methanotroph droplets. Gray areas mark difference between control and CO2 control means. Asterisks indicate significant difference from controls, diamonds indicate significant difference from CO2 controls (pairwise comparisons against controls, Mann–Whitney U-test, * = ≤ 0.05, ** ≤ 0.01). bd below detection, na not applicable

Article Snippet: After incubation at 20% CH 4 until growth developed (5–7 days), we quantified the cell biomass and methane oxidation rates. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 1 caption a7 Biomass ( a , b ) and population methane oxidation ( c , d ) of the methanotrophs Methylobacter luteus and Methylocystis parvus grown on two-compartment agar plates at 20% CH 4 headspace, incubated alone (Control), alone with 5% CO (CO control) or in the presence of a heterotroph: Bacillus pumilus isolate YXY-10, Bacillus simplex strain DUCC3713, Stenotrophomonas maltophilia strain ATCC 13637, Exiguobacterium undae strain B111, Pseudomonas mandelii JR-1.

Techniques: Incubation, Control, MANN-WHITNEY