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    BIOLOG Life Science Institute 8 fca
    Time course of <t>8-FcA</t> accumulation in CHO cells stably expressing the vector, OATP1B1, or OATP1B3. The 8-FcA concentration for CHO vector and CHO OATP1B1 (A) was 2.5 μM and 1.25 μM for CHO vector and CHO OATP1B3 (B). Data represent the mean
    8 Fca, supplied by BIOLOG Life Science Institute, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Time course of 8-FcA accumulation in CHO cells stably expressing the vector, OATP1B1, or OATP1B3. The 8-FcA concentration for CHO vector and CHO OATP1B1 (A) was 2.5 μM and 1.25 μM for CHO vector and CHO OATP1B3 (B). Data represent the mean

    Journal: Analytical biochemistry

    Article Title: Fluorescence-Based Assays for the Assessment of Drug Interaction with the Human Transporters OATP1B1 and OATP1B3

    doi: 10.1016/j.ab.2010.06.012

    Figure Lengend Snippet: Time course of 8-FcA accumulation in CHO cells stably expressing the vector, OATP1B1, or OATP1B3. The 8-FcA concentration for CHO vector and CHO OATP1B1 (A) was 2.5 μM and 1.25 μM for CHO vector and CHO OATP1B3 (B). Data represent the mean

    Article Snippet: 8-FcA was purchased from Biolog Life Science Institute (Bremen, Germany) via its American distributor Axxora, LLC (San Diego, CA).

    Techniques: Stable Transfection, Expressing, Plasmid Preparation, Concentration Assay

    The IC 50 determination for cyclosporin A ( open symbols ) and rifampicin ( filled symbols ) versus 8-FcA for OATP1B1 (A) and OATP1B3 (B). The IC 50 values for cyclosporine A and rifampicin against OATP1B1 mediated transport were 0.25 μM, and 0.99 μM,

    Journal: Analytical biochemistry

    Article Title: Fluorescence-Based Assays for the Assessment of Drug Interaction with the Human Transporters OATP1B1 and OATP1B3

    doi: 10.1016/j.ab.2010.06.012

    Figure Lengend Snippet: The IC 50 determination for cyclosporin A ( open symbols ) and rifampicin ( filled symbols ) versus 8-FcA for OATP1B1 (A) and OATP1B3 (B). The IC 50 values for cyclosporine A and rifampicin against OATP1B1 mediated transport were 0.25 μM, and 0.99 μM,

    Article Snippet: 8-FcA was purchased from Biolog Life Science Institute (Bremen, Germany) via its American distributor Axxora, LLC (San Diego, CA).

    Techniques:

    The five minute cellular accumulation of 2 μM estrone-3-sulfate (A), bromosulfophthalein (BSP) (B), dehydroepiandrosterone sulfate (DHEAS) (C), and 8-FcA (D) by CHO vector , CHO OATP1B1 , CHO OATP1B3 , and CHO OATP2B1 cells. Data is expressed relative

    Journal: Analytical biochemistry

    Article Title: Fluorescence-Based Assays for the Assessment of Drug Interaction with the Human Transporters OATP1B1 and OATP1B3

    doi: 10.1016/j.ab.2010.06.012

    Figure Lengend Snippet: The five minute cellular accumulation of 2 μM estrone-3-sulfate (A), bromosulfophthalein (BSP) (B), dehydroepiandrosterone sulfate (DHEAS) (C), and 8-FcA (D) by CHO vector , CHO OATP1B1 , CHO OATP1B3 , and CHO OATP2B1 cells. Data is expressed relative

    Article Snippet: 8-FcA was purchased from Biolog Life Science Institute (Bremen, Germany) via its American distributor Axxora, LLC (San Diego, CA).

    Techniques: Plasmid Preparation

    The two-hour cellular accumulation when exposed to 0.1 μM bis-POM-PMEA ( solid white and solid black bars ) or 1.25 μM 8-FcA (diagonal black stripe on white background and diagonal white stripe on black background) in CHO WT , CHO vector , CHO

    Journal: Analytical biochemistry

    Article Title: Fluorescence-Based Assays for the Assessment of Drug Interaction with the Human Transporters OATP1B1 and OATP1B3

    doi: 10.1016/j.ab.2010.06.012

    Figure Lengend Snippet: The two-hour cellular accumulation when exposed to 0.1 μM bis-POM-PMEA ( solid white and solid black bars ) or 1.25 μM 8-FcA (diagonal black stripe on white background and diagonal white stripe on black background) in CHO WT , CHO vector , CHO

    Article Snippet: 8-FcA was purchased from Biolog Life Science Institute (Bremen, Germany) via its American distributor Axxora, LLC (San Diego, CA).

    Techniques: Plasmid Preparation

    The concentration dependent transport of 8-FcA by OATP1B1 (A) and OATP1B3 (B). The 8-FcA concentration ranged from 0.25 μM to 25 μM and the period of uptake for each transporter was 10 minutes. The K m and V max for each transporter is indicated

    Journal: Analytical biochemistry

    Article Title: Fluorescence-Based Assays for the Assessment of Drug Interaction with the Human Transporters OATP1B1 and OATP1B3

    doi: 10.1016/j.ab.2010.06.012

    Figure Lengend Snippet: The concentration dependent transport of 8-FcA by OATP1B1 (A) and OATP1B3 (B). The 8-FcA concentration ranged from 0.25 μM to 25 μM and the period of uptake for each transporter was 10 minutes. The K m and V max for each transporter is indicated

    Article Snippet: 8-FcA was purchased from Biolog Life Science Institute (Bremen, Germany) via its American distributor Axxora, LLC (San Diego, CA).

    Techniques: Concentration Assay

    The cellular accumulation of 8-FcA in transiently transfected CHO cells following a 20 minute exposure to 10 μM 8-FcA. The expressed transporter for each image is as follows: untransfected CHO cells (A), control vector (B), NTCP (C), OAT1 (D),

    Journal: Analytical biochemistry

    Article Title: Fluorescence-Based Assays for the Assessment of Drug Interaction with the Human Transporters OATP1B1 and OATP1B3

    doi: 10.1016/j.ab.2010.06.012

    Figure Lengend Snippet: The cellular accumulation of 8-FcA in transiently transfected CHO cells following a 20 minute exposure to 10 μM 8-FcA. The expressed transporter for each image is as follows: untransfected CHO cells (A), control vector (B), NTCP (C), OAT1 (D),

    Article Snippet: 8-FcA was purchased from Biolog Life Science Institute (Bremen, Germany) via its American distributor Axxora, LLC (San Diego, CA).

    Techniques: Transfection, Plasmid Preparation

    Inhibition of 8-FcA Transport

    Journal: Analytical biochemistry

    Article Title: Fluorescence-Based Assays for the Assessment of Drug Interaction with the Human Transporters OATP1B1 and OATP1B3

    doi: 10.1016/j.ab.2010.06.012

    Figure Lengend Snippet: Inhibition of 8-FcA Transport

    Article Snippet: 8-FcA was purchased from Biolog Life Science Institute (Bremen, Germany) via its American distributor Axxora, LLC (San Diego, CA).

    Techniques: Inhibition