Journal: bioRxiv

Article Title: Selective inhibition of fibroblast-specific Domain Discoidin Receptor 1 (DDR1) reduces collagen deposition and modulates fibroblast-specific cytokine release within the breast microenvironment

doi: 10.1101/2023.08.11.552937

Figure Lengend Snippet: RMF-EG were cultured in 3D collagen gels in the presence of DDR1i (+) or vehicle (−) followed by assessment of collagen gel contraction [n=3 gels per condition] (A). Reduced gel contraction with DDR1i was observed (A). RMF-EG were cultured on collagen-coated tissue culture plate or in 3D hydrogel, followed by RNA isolation. RT-qPCR gene expression analysis demonstrated that DDR1 inhibition reduces expression of CAF markers SMA, FAP in the 2D or 3D context (B). IL-6 was upregulated in RMF-EG cultured in 3D and downregulated with DDR1 inhibition (B). Cell supernatents from RMF-EG laden collagen gels were collected for multiplex human cy1okine array analysis. We identified high secretion of IL-6, MCP-1, and IL-8 which were reduced with DDR1 inhibition (C). To confirm this finding, RNA was isolated from RMF-EG cultured in 3D hydrogel followed by RT-qPCR gene expression analysis. RMF-EG in 3D hydrogels showed higher expression of IL-8, MCP-1, and Col1a1 compared to 2D, subsequently reduced with DDR1 inhibition (D). * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.

Article Snippet: Eight-week-old female C57BL/6J mice (Jackson Laboratories) were administered 7rh DDR1 inhibitor (Tocris 5860) 25 mg/kg or vehicle control (1% carboxymethylcellulose [Fisher Scientific C5678], 0.25% Tween 80 [Millipore Sigma P1754]) via oral gavage for 14 days.

Techniques: Cell Culture, Isolation, Quantitative RT-PCR, Gene Expression, Inhibition, Expressing, Multiplex Assay

Journal: bioRxiv

Article Title: Selective inhibition of fibroblast-specific Domain Discoidin Receptor 1 (DDR1) reduces collagen deposition and modulates fibroblast-specific cytokine release within the breast microenvironment

doi: 10.1101/2023.08.11.552937

Figure Lengend Snippet: Dose titration of DOR1 inhibitors to assess cell viability and effective DDR1 inhibition

Article Snippet: Eight-week-old female C57BL/6J mice (Jackson Laboratories) were administered 7rh DDR1 inhibitor (Tocris 5860) 25 mg/kg or vehicle control (1% carboxymethylcellulose [Fisher Scientific C5678], 0.25% Tween 80 [Millipore Sigma P1754]) via oral gavage for 14 days.

Techniques: Titration, Inhibition

Journal: Cell Reports Medicine

Article Title: Loss of p53 and SMAD4 induces adenosquamous subtype pancreatic cancer in the absence of an oncogenic KRAS mutation

doi: 10.1016/j.xcrm.2024.101711

Figure Lengend Snippet:

Article Snippet: 7RH , MedChemExpress , Cat# HY-U00444.

Techniques: Virus, Recombinant, Protease Inhibitor, Reverse Transcription, SYBR Green Assay, CCK-8 Assay, Plasmid Preparation, Staining, Bicinchoninic Acid Protein Assay, Sample Prep, Expressing, Software