7h9 liquid medium Search Results


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  • 99
    Millipore oadc growth supplement
    Oadc Growth Supplement, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco 7h9 liquid medium
    Growth of M. smegmatis in various nitrogen sources. Wild-type SMR5 and glnR deletion strain MH1 were grown in the presence of the indicated substances (10 mM final concentration) as sole nitrogen source. Standard <t>7H9</t> medium was used as positive control; 7H9 lacking any nitrogen source as negative control (7H9-N). (A) Putative nitrogen sources deduced from microarray results, (B) putative nitrogen sources used by closely related species.
    7h9 Liquid Medium, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson 7h9 liquid medium
    Growth curves of the WT, ΔMycP 3 mutant and the two complementation strains, ΔMycP3 ms ::pr1MycP3 ms and ΔMycP3 ms ::pr2MycP3 ms under Fe-free <t>7H9</t> ( a ), Fe-free Sauton’s media ( b ). The growth curves were done in triplicate, error bars show standard deviation
    7h9 Liquid Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7h9 liquid medium
    Growth curves of the WT, ΔMycP 3 mutant and the two complementation strains, ΔMycP3 ms ::pr1MycP3 ms and ΔMycP3 ms ::pr2MycP3 ms under Fe-free <t>7H9</t> ( a ), Fe-free Sauton’s media ( b ). The growth curves were done in triplicate, error bars show standard deviation
    7h9 Liquid Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 7h9 liquid medium
    Growth of RGM within A. polyphaga trophozoites. M. gilvum (A), M. rhodesiae (B), and M. thermoresistibile (C) were cocultured with the free-living amoeba A. polyphaga (black bars), cultivated in PAS medium (gray bars), and cultivated in <t>7H9</t> complete medium
    7h9 Liquid Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals 7h9 liquid medium
    Growth of M. tuberculosis strains in liquid and solid media. (A) Wild-type M. tuberculosis H37Rv, Δ cuvA Mt , and complemented Δ cuvA (Δ cuvA Mt /C) strains were grown in <t>7H9-ADC-Tw</t> liquid medium with 0.2% glucose, and the OD 600 was measured daily. (B) Growth of the same strains on agar plates containing MM plus 0.2% glucose or MM plus 0.01% cholesterol. Serial 10-fold dilutions were spotted and incubated at 37°C, and photographs were taken after 1 month of incubation. In both panels, data shown are from one experiment that was repeated at least twice with similar results. Glc, 0.2% glucose; Chol., 0.01% cholesterol.
    7h9 Liquid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 99/100, based on 629 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco 7h9 liquid growth medium
    Growth of M. tuberculosis strains in liquid and solid media. (A) Wild-type M. tuberculosis H37Rv, Δ cuvA Mt , and complemented Δ cuvA (Δ cuvA Mt /C) strains were grown in <t>7H9-ADC-Tw</t> liquid medium with 0.2% glucose, and the OD 600 was measured daily. (B) Growth of the same strains on agar plates containing MM plus 0.2% glucose or MM plus 0.01% cholesterol. Serial 10-fold dilutions were spotted and incubated at 37°C, and photographs were taken after 1 month of incubation. In both panels, data shown are from one experiment that was repeated at least twice with similar results. Glc, 0.2% glucose; Chol., 0.01% cholesterol.
    7h9 Liquid Growth Medium, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore middlebrooks 7h9 liquid medium
    Growth of M. tuberculosis strains in liquid and solid media. (A) Wild-type M. tuberculosis H37Rv, Δ cuvA Mt , and complemented Δ cuvA (Δ cuvA Mt /C) strains were grown in <t>7H9-ADC-Tw</t> liquid medium with 0.2% glucose, and the OD 600 was measured daily. (B) Growth of the same strains on agar plates containing MM plus 0.2% glucose or MM plus 0.01% cholesterol. Serial 10-fold dilutions were spotted and incubated at 37°C, and photographs were taken after 1 month of incubation. In both panels, data shown are from one experiment that was repeated at least twice with similar results. Glc, 0.2% glucose; Chol., 0.01% cholesterol.
    Middlebrooks 7h9 Liquid Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore middelbrook 7h9 liquid medium
    Growth of M. tuberculosis strains in liquid and solid media. (A) Wild-type M. tuberculosis H37Rv, Δ cuvA Mt , and complemented Δ cuvA (Δ cuvA Mt /C) strains were grown in <t>7H9-ADC-Tw</t> liquid medium with 0.2% glucose, and the OD 600 was measured daily. (B) Growth of the same strains on agar plates containing MM plus 0.2% glucose or MM plus 0.01% cholesterol. Serial 10-fold dilutions were spotted and incubated at 37°C, and photographs were taken after 1 month of incubation. In both panels, data shown are from one experiment that was repeated at least twice with similar results. Glc, 0.2% glucose; Chol., 0.01% cholesterol.
    Middelbrook 7h9 Liquid Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco 7h9 basal liquid medium
    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook <t>7H9</t> broth with glycerol. The optical density at 600
    7h9 Basal Liquid Medium, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals 7h9 liquid medium oadc
    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook <t>7H9</t> broth with glycerol. The optical density at 600
    7h9 Liquid Medium Oadc, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals 7h9 liquid medium preparation middlebrook 7h9 broth
    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook <t>7H9</t> broth with glycerol. The optical density at 600
    7h9 Liquid Medium Preparation Middlebrook 7h9 Broth, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals dmso middlebrook 7h9 liquid medium
    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook <t>7H9</t> broth with glycerol. The optical density at 600
    Dmso Middlebrook 7h9 Liquid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals dtt middlebrook 7h9 liquid medium complete
    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook <t>7H9</t> broth with glycerol. The optical density at 600
    Dtt Middlebrook 7h9 Liquid Medium Complete, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals dubos oleic albumin complex enriched middlebrook 7h9 liquid medium
    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook <t>7H9</t> broth with glycerol. The optical density at 600
    Dubos Oleic Albumin Complex Enriched Middlebrook 7h9 Liquid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals 7h9 liquid medium plus albumin dextrose catalase adc
    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook <t>7H9</t> broth with glycerol. The optical density at 600
    7h9 Liquid Medium Plus Albumin Dextrose Catalase Adc, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals medium
    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook <t>7H9</t> broth with glycerol. The optical density at 600
    Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals oleic acid dextrose catalase oadc enriched middlebrook 7h9 liquid medium
    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook <t>7H9</t> broth with glycerol. The optical density at 600
    Oleic Acid Dextrose Catalase Oadc Enriched Middlebrook 7h9 Liquid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco 7h9 medium
    —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid <t>7H9</t> medium supplemented with 0.05% Tween 80, ADC supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).
    7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 95/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals 7h9 medium
    —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid <t>7H9</t> medium supplemented with 0.05% Tween 80, ADC supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).
    7h9 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 95/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson 7h9 oadc medium
    —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid <t>7H9</t> medium supplemented with 0.05% Tween 80, ADC supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).
    7h9 Oadc Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco 7h10 solid medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    7h10 Solid Medium, supplied by Difco, used in various techniques. Bioz Stars score: 91/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson middlebrook 7h9 medium
    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook <t>7H10</t> plates and incubated at 37 °C for 21 days.
    Middlebrook 7h9 Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 97/100, based on 259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h9 medium
    Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in <t>Middlebrook</t> <t>7H9</t> medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation
    Middlebrook 7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 95/100, based on 835 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore middlebrook 7h9 medium
    Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in <t>Middlebrook</t> <t>7H9</t> medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation
    Middlebrook 7h9 Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson 7h9 medium
    Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in <t>Middlebrook</t> <t>7H9</t> medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation
    7h9 Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals middlebrook 7h9 medium
    In vitro growth kinetics of M. tuberculosis Erdman wild type and the five rpf deletion mutant strains in <t>Middlebrook</t> <t>7H9</t> medium at 37°C with agitation. The ΔRv1009 strain used in this experiment was mc 2 3126. We also found that strain mc
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    MiddleBrook Pharmaceuticals 7h9 oadc medium
    In vitro growth kinetics of M. tuberculosis Erdman wild type and the five rpf deletion mutant strains in <t>Middlebrook</t> <t>7H9</t> medium at 37°C with agitation. The ΔRv1009 strain used in this experiment was mc 2 3126. We also found that strain mc
    7h9 Oadc Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middelbrook 7h9 medium
    In vitro growth kinetics of M. tuberculosis Erdman wild type and the five rpf deletion mutant strains in <t>Middlebrook</t> <t>7H9</t> medium at 37°C with agitation. The ΔRv1009 strain used in this experiment was mc 2 3126. We also found that strain mc
    Middelbrook 7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals 7h10 solid medium
    In vitro growth kinetics of M. tuberculosis Erdman wild type and the five rpf deletion mutant strains in <t>Middlebrook</t> <t>7H9</t> medium at 37°C with agitation. The ΔRv1009 strain used in this experiment was mc 2 3126. We also found that strain mc
    7h10 Solid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 95/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco middlebrook 7h9 oadc medium
    In vitro growth kinetics of M. tuberculosis Erdman wild type and the five rpf deletion mutant strains in <t>Middlebrook</t> <t>7H9</t> medium at 37°C with agitation. The ΔRv1009 strain used in this experiment was mc 2 3126. We also found that strain mc
    Middlebrook 7h9 Oadc Medium, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiddleBrook Pharmaceuticals medium modified middlebrook 7h9 broth
    In vitro growth kinetics of M. tuberculosis Erdman wild type and the five rpf deletion mutant strains in <t>Middlebrook</t> <t>7H9</t> medium at 37°C with agitation. The ΔRv1009 strain used in this experiment was mc 2 3126. We also found that strain mc
    Medium Modified Middlebrook 7h9 Broth, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Growth of M. smegmatis in various nitrogen sources. Wild-type SMR5 and glnR deletion strain MH1 were grown in the presence of the indicated substances (10 mM final concentration) as sole nitrogen source. Standard 7H9 medium was used as positive control; 7H9 lacking any nitrogen source as negative control (7H9-N). (A) Putative nitrogen sources deduced from microarray results, (B) putative nitrogen sources used by closely related species.

    Journal: BMC Research Notes

    Article Title: Nitrogen starvation-induced transcriptome alterations and influence of transcription regulator mutants in Mycobacterium smegmatis

    doi: 10.1186/1756-0500-6-482

    Figure Lengend Snippet: Growth of M. smegmatis in various nitrogen sources. Wild-type SMR5 and glnR deletion strain MH1 were grown in the presence of the indicated substances (10 mM final concentration) as sole nitrogen source. Standard 7H9 medium was used as positive control; 7H9 lacking any nitrogen source as negative control (7H9-N). (A) Putative nitrogen sources deduced from microarray results, (B) putative nitrogen sources used by closely related species.

    Article Snippet: Mycobacterial strains were grown in Middlebrook 7H9 liquid medium (Difco Laboratories; per 900 ml approx.

    Techniques: Concentration Assay, Positive Control, Negative Control, Microarray

    Mass spectrometry analysis of MAs. MAs were extracted from mid-log-phase liquid 7H9 medium cultures. Samples were analyzed via a QTRAP 4000 mass spectrometer. (A) Individual sums of C 26 α-, C 24 α-, C 26 keto-, and C 24 keto-MA profiles in

    Journal: Infection and Immunity

    Article Title: An ethA-ethR-Deficient Mycobacterium bovis BCG Mutant Displays Increased Adherence to Mammalian Cells and Greater Persistence In Vivo, Which Correlate with Altered Mycolic Acid Composition

    doi: 10.1128/IAI.01332-13

    Figure Lengend Snippet: Mass spectrometry analysis of MAs. MAs were extracted from mid-log-phase liquid 7H9 medium cultures. Samples were analyzed via a QTRAP 4000 mass spectrometer. (A) Individual sums of C 26 α-, C 24 α-, C 26 keto-, and C 24 keto-MA profiles in

    Article Snippet: Wild-type (WT) M. bovis BCG (Pasteur strain ATCC 35734) and derivative strains were grown at 37°C in Middlebrook liquid 7H9 medium (Difco) or on 7H11 agar supplemented with ADS (0.5% bovine serum albumin fraction V, 0.2% dextrose, 0.85% saline) enrichment, 0.05% Tween 80, and 0.2% glycerol with appropriate antibiotics (80 μg/ml hygromycin [Roche], 20 μg/ml kanamycin [Sigma]).

    Techniques: Mass Spectrometry

    Expression of S18 mRNAs and proteins in M. tuberculosis H37Rv during growth in 7H9/ADC vs. Sauton's medium

    Journal: Molecular microbiology

    Article Title: Zinc Regulates a Switch between Primary and Alternative S18 Ribosomal Proteins in Mycobacterium tuberculosis

    doi: 10.1111/mmi.13022

    Figure Lengend Snippet: Expression of S18 mRNAs and proteins in M. tuberculosis H37Rv during growth in 7H9/ADC vs. Sauton's medium

    Article Snippet: M. tuberculosis H37Rv was grown either in Middlebrook 7H9 liquid culture medium (Difco) supplemented with ADC (0.5% albumin, 0.2% glucose, 0.085% NaCl and 0.05% Tween 80) or Sauton's medium prepared without zinc salts (0.05% KH2 PO4 , 0.05% MgSO4 7H2 O, 0.2% citric acid, 0.005% ferric ammonium citrate, 6% glycerol, 0.4% Asparagine, 0.05% Tween 80, pH 7.4).

    Techniques: Expressing

    Growth curves of the WT, ΔMycP 3 mutant and the two complementation strains, ΔMycP3 ms ::pr1MycP3 ms and ΔMycP3 ms ::pr2MycP3 ms under Fe-free 7H9 ( a ), Fe-free Sauton’s media ( b ). The growth curves were done in triplicate, error bars show standard deviation

    Journal: BMC Research Notes

    Article Title: Two promoters in the esx-3 gene cluster of Mycobacterium smegmatis respond inversely to different iron concentrations in vitro

    doi: 10.1186/s13104-017-2752-0

    Figure Lengend Snippet: Growth curves of the WT, ΔMycP 3 mutant and the two complementation strains, ΔMycP3 ms ::pr1MycP3 ms and ΔMycP3 ms ::pr2MycP3 ms under Fe-free 7H9 ( a ), Fe-free Sauton’s media ( b ). The growth curves were done in triplicate, error bars show standard deviation

    Article Snippet: M. smegmatis was cultured using both Middlebrook 7H9 liquid medium (Becton–Dickinson, USA, Catalogue No. 221832) and Difco 7H11 solid medium (Becton–Dickinson, USA, Catalogue No. 212304) both supplemented with 0.05% (v/v) Tween 80 (Sigma Aldrich, USA, Catalogue No. P1754), 0.5% (w/v) glucose (Sigma Aldrich, USA, Catalogue No. 47829), and 0.5% (v/v) glycerol (Sigma-Aldrich, USA, Catalogue No. G5516).

    Techniques: Mutagenesis, Mass Spectrometry, Standard Deviation

    The comparison of the intracellular iron levels in WT ms , ΔMycP3 ms , ΔMycP3 ms ::pr1MycP3 ms and ΔMycP3 ms ::pr2MycP3 ms strains under 7H9, Fe-depleted 7H9 and Fe rescued 7H9 media. The error bars show standard error of the mean (n = 4). The p values obtained using two-way ANOVA statistical analysis between different culturing conditions for all four strains are smaller than 0.0001 ( *** ), an example is shown for the WT ms

    Journal: BMC Research Notes

    Article Title: Two promoters in the esx-3 gene cluster of Mycobacterium smegmatis respond inversely to different iron concentrations in vitro

    doi: 10.1186/s13104-017-2752-0

    Figure Lengend Snippet: The comparison of the intracellular iron levels in WT ms , ΔMycP3 ms , ΔMycP3 ms ::pr1MycP3 ms and ΔMycP3 ms ::pr2MycP3 ms strains under 7H9, Fe-depleted 7H9 and Fe rescued 7H9 media. The error bars show standard error of the mean (n = 4). The p values obtained using two-way ANOVA statistical analysis between different culturing conditions for all four strains are smaller than 0.0001 ( *** ), an example is shown for the WT ms

    Article Snippet: M. smegmatis was cultured using both Middlebrook 7H9 liquid medium (Becton–Dickinson, USA, Catalogue No. 221832) and Difco 7H11 solid medium (Becton–Dickinson, USA, Catalogue No. 212304) both supplemented with 0.05% (v/v) Tween 80 (Sigma Aldrich, USA, Catalogue No. P1754), 0.5% (w/v) glucose (Sigma Aldrich, USA, Catalogue No. 47829), and 0.5% (v/v) glycerol (Sigma-Aldrich, USA, Catalogue No. G5516).

    Techniques: Mass Spectrometry

    Growth curves of the WT, ΔMycP 3 mutant and the two complementation strains, ΔMycP3 ms ::pr1MycP3 ms and ΔMycP3 ms ::pr2MycP3 ms under Fe-depleted 7H9 ( a ), and Fe-depleted Sauton’s media ( b ). The growth curves were done in triplicate, error bars show standard deviation

    Journal: BMC Research Notes

    Article Title: Two promoters in the esx-3 gene cluster of Mycobacterium smegmatis respond inversely to different iron concentrations in vitro

    doi: 10.1186/s13104-017-2752-0

    Figure Lengend Snippet: Growth curves of the WT, ΔMycP 3 mutant and the two complementation strains, ΔMycP3 ms ::pr1MycP3 ms and ΔMycP3 ms ::pr2MycP3 ms under Fe-depleted 7H9 ( a ), and Fe-depleted Sauton’s media ( b ). The growth curves were done in triplicate, error bars show standard deviation

    Article Snippet: M. smegmatis was cultured using both Middlebrook 7H9 liquid medium (Becton–Dickinson, USA, Catalogue No. 221832) and Difco 7H11 solid medium (Becton–Dickinson, USA, Catalogue No. 212304) both supplemented with 0.05% (v/v) Tween 80 (Sigma Aldrich, USA, Catalogue No. P1754), 0.5% (w/v) glucose (Sigma Aldrich, USA, Catalogue No. 47829), and 0.5% (v/v) glycerol (Sigma-Aldrich, USA, Catalogue No. G5516).

    Techniques: Mutagenesis, Mass Spectrometry, Standard Deviation

    Gene expression analysis of mycP 3 in WT ms , ΔMycP 3ms , ΔMycP 3ms ::Pr1MycP 3 , and ΔMycP 3ms ::Pr2MycP 3 strains under normal 7H9 (iron rich), Fe-free 7H9, and Fe-rescued 7H9 media. The results were normalized against the RNA copy number of sigA . The p values obtained using two-way ANOVA statistical analysis (n = 3) (*p

    Journal: BMC Research Notes

    Article Title: Two promoters in the esx-3 gene cluster of Mycobacterium smegmatis respond inversely to different iron concentrations in vitro

    doi: 10.1186/s13104-017-2752-0

    Figure Lengend Snippet: Gene expression analysis of mycP 3 in WT ms , ΔMycP 3ms , ΔMycP 3ms ::Pr1MycP 3 , and ΔMycP 3ms ::Pr2MycP 3 strains under normal 7H9 (iron rich), Fe-free 7H9, and Fe-rescued 7H9 media. The results were normalized against the RNA copy number of sigA . The p values obtained using two-way ANOVA statistical analysis (n = 3) (*p

    Article Snippet: M. smegmatis was cultured using both Middlebrook 7H9 liquid medium (Becton–Dickinson, USA, Catalogue No. 221832) and Difco 7H11 solid medium (Becton–Dickinson, USA, Catalogue No. 212304) both supplemented with 0.05% (v/v) Tween 80 (Sigma Aldrich, USA, Catalogue No. P1754), 0.5% (w/v) glucose (Sigma Aldrich, USA, Catalogue No. 47829), and 0.5% (v/v) glycerol (Sigma-Aldrich, USA, Catalogue No. G5516).

    Techniques: Expressing, Mass Spectrometry

    Growth of RGM within A. polyphaga trophozoites. M. gilvum (A), M. rhodesiae (B), and M. thermoresistibile (C) were cocultured with the free-living amoeba A. polyphaga (black bars), cultivated in PAS medium (gray bars), and cultivated in 7H9 complete medium

    Journal: Applied and Environmental Microbiology

    Article Title: Mycobacterium gilvum Illustrates Size-Correlated Relationships between Mycobacteria and Acanthamoeba polyphaga

    doi: 10.1128/AEM.03765-12

    Figure Lengend Snippet: Growth of RGM within A. polyphaga trophozoites. M. gilvum (A), M. rhodesiae (B), and M. thermoresistibile (C) were cocultured with the free-living amoeba A. polyphaga (black bars), cultivated in PAS medium (gray bars), and cultivated in 7H9 complete medium

    Article Snippet: Mycobacteria were cultured in Middlebrook 7H9 liquid medium (Sigma-Aldrich, Lyon, France) and subcultured at 37°C on Middlebrook and Cohn 7H10 agar (Becton, Dickinson, Le Pont de Claix, France) for 3 days.

    Techniques:

    Mycobacterial size in 7H9 medium. The sizes of M. gilvum (A), M. senegalense (B), M. conceptionense (C), M. rhodesiae (D), M. thermoresistibile (E), M. chelonae (F), M. smegmatis (G), M. abscessus (H), and M. fortuitum subsp. fortuitum (I) were determined

    Journal: Applied and Environmental Microbiology

    Article Title: Mycobacterium gilvum Illustrates Size-Correlated Relationships between Mycobacteria and Acanthamoeba polyphaga

    doi: 10.1128/AEM.03765-12

    Figure Lengend Snippet: Mycobacterial size in 7H9 medium. The sizes of M. gilvum (A), M. senegalense (B), M. conceptionense (C), M. rhodesiae (D), M. thermoresistibile (E), M. chelonae (F), M. smegmatis (G), M. abscessus (H), and M. fortuitum subsp. fortuitum (I) were determined

    Article Snippet: Mycobacteria were cultured in Middlebrook 7H9 liquid medium (Sigma-Aldrich, Lyon, France) and subcultured at 37°C on Middlebrook and Cohn 7H10 agar (Becton, Dickinson, Le Pont de Claix, France) for 3 days.

    Techniques:

    Growth of M. tuberculosis strains in liquid and solid media. (A) Wild-type M. tuberculosis H37Rv, Δ cuvA Mt , and complemented Δ cuvA (Δ cuvA Mt /C) strains were grown in 7H9-ADC-Tw liquid medium with 0.2% glucose, and the OD 600 was measured daily. (B) Growth of the same strains on agar plates containing MM plus 0.2% glucose or MM plus 0.01% cholesterol. Serial 10-fold dilutions were spotted and incubated at 37°C, and photographs were taken after 1 month of incubation. In both panels, data shown are from one experiment that was repeated at least twice with similar results. Glc, 0.2% glucose; Chol., 0.01% cholesterol.

    Journal: Infection and Immunity

    Article Title: Mycobacterial Gene cuvA Is Required for Optimal Nutrient Utilization and Virulence

    doi: 10.1128/IAI.02207-14

    Figure Lengend Snippet: Growth of M. tuberculosis strains in liquid and solid media. (A) Wild-type M. tuberculosis H37Rv, Δ cuvA Mt , and complemented Δ cuvA (Δ cuvA Mt /C) strains were grown in 7H9-ADC-Tw liquid medium with 0.2% glucose, and the OD 600 was measured daily. (B) Growth of the same strains on agar plates containing MM plus 0.2% glucose or MM plus 0.01% cholesterol. Serial 10-fold dilutions were spotted and incubated at 37°C, and photographs were taken after 1 month of incubation. In both panels, data shown are from one experiment that was repeated at least twice with similar results. Glc, 0.2% glucose; Chol., 0.01% cholesterol.

    Article Snippet: For measurements of growth on solid medium, cells grown in Middlebrook 7H9 liquid medium to an optical density at 600 nm (OD600 ) of 0.5 were washed in phosphate-buffered saline containing 0.05% tyloxapol (PBS-Tx), and 10-fold serial dilutions in PBS were spotted on agar plates containing MM as described above (without tyloxapol) plus a 0.01% concentration of the carbon source.

    Techniques: Incubation, Gas Chromatography

    Growth of MS_Vec and MS_Rv3402c under stress conditions. (A). Growth of MS-Vec and MS-Rv3402c at 37°C in MB 7H9 liquid medium was monitored by determining OD 600 at intervals of 3 h. (B). Growth curve of MS-Vec and MS-Rv3402c in iron-depleted medium. Two recombinant M. smegmatis were grown in MB 7H9 medium supplemented with 100 μM 2′ 2′ dipyridyl. The growth of the strains were monitored by measuring OD 600 at intervals of 6 h. (C). In vitro growth of MS-Vec and MS-Rv3402c at different pH. The bacteria were collected by centrifugation and resuspended to an OD 600 of 0.5 in 5 ml MB 7H9 (pH 5 or 3). All cultures were again incubated at 37°C and 0.1 ml removed for viable count enumeration after 0, 3 and 6 h. (D). Survival of recombinant M. smegmatis after exposure to hydrogen peroxide. Aliquots (5 ml) of cultures at OD 600 of 0.5 were exposed to 5 mM hydrogen peroxide (H 2 O 2 ) for 6 h at 37°C. The cultures were serially diluted and plated onto MB 7H10 plates and the colonies counted after 3–4 days of incubation at 37°C.

    Journal: PLoS ONE

    Article Title: Mycobacterium tuberculosis Rv3402c Enhances Mycobacterial Survival within Macrophages and Modulates the Host Pro-Inflammatory Cytokines Production via NF-Kappa B/ERK/p38 Signaling

    doi: 10.1371/journal.pone.0094418

    Figure Lengend Snippet: Growth of MS_Vec and MS_Rv3402c under stress conditions. (A). Growth of MS-Vec and MS-Rv3402c at 37°C in MB 7H9 liquid medium was monitored by determining OD 600 at intervals of 3 h. (B). Growth curve of MS-Vec and MS-Rv3402c in iron-depleted medium. Two recombinant M. smegmatis were grown in MB 7H9 medium supplemented with 100 μM 2′ 2′ dipyridyl. The growth of the strains were monitored by measuring OD 600 at intervals of 6 h. (C). In vitro growth of MS-Vec and MS-Rv3402c at different pH. The bacteria were collected by centrifugation and resuspended to an OD 600 of 0.5 in 5 ml MB 7H9 (pH 5 or 3). All cultures were again incubated at 37°C and 0.1 ml removed for viable count enumeration after 0, 3 and 6 h. (D). Survival of recombinant M. smegmatis after exposure to hydrogen peroxide. Aliquots (5 ml) of cultures at OD 600 of 0.5 were exposed to 5 mM hydrogen peroxide (H 2 O 2 ) for 6 h at 37°C. The cultures were serially diluted and plated onto MB 7H10 plates and the colonies counted after 3–4 days of incubation at 37°C.

    Article Snippet: M. smegmatis mc2 155 was grown at 37°C in Middlebrook (MB) 7H9 liquid medium or on MB 7H10 agar supplemented with 0.2% (w/v) glucose, 0.5% (v/v) glycerol and 0.05% (v/v) Tween 80.

    Techniques: Mass Spectrometry, Recombinant, In Vitro, Centrifugation, Incubation

    Expression of Rv3402c in recombinant M. smegmatis . (A). Ms_Rv3402c and Ms_Vec were grown at 37°C in MB 7H9 liquid medium to an OD 600 of 0.6–1.0. Total bacterial RNA was isolated after 16 h induction and subjected to RT-PCR to detect expression of the rv3402c and rrsB genes. (B). Lysates were prepared from bacterial cells that were cultured as in (A) and subjected to Western blot analysis to detect Myc-tagged Rv3402c protein using mouse anti-Myc antibody.

    Journal: PLoS ONE

    Article Title: Mycobacterium tuberculosis Rv3402c Enhances Mycobacterial Survival within Macrophages and Modulates the Host Pro-Inflammatory Cytokines Production via NF-Kappa B/ERK/p38 Signaling

    doi: 10.1371/journal.pone.0094418

    Figure Lengend Snippet: Expression of Rv3402c in recombinant M. smegmatis . (A). Ms_Rv3402c and Ms_Vec were grown at 37°C in MB 7H9 liquid medium to an OD 600 of 0.6–1.0. Total bacterial RNA was isolated after 16 h induction and subjected to RT-PCR to detect expression of the rv3402c and rrsB genes. (B). Lysates were prepared from bacterial cells that were cultured as in (A) and subjected to Western blot analysis to detect Myc-tagged Rv3402c protein using mouse anti-Myc antibody.

    Article Snippet: M. smegmatis mc2 155 was grown at 37°C in Middlebrook (MB) 7H9 liquid medium or on MB 7H10 agar supplemented with 0.2% (w/v) glucose, 0.5% (v/v) glycerol and 0.05% (v/v) Tween 80.

    Techniques: Expressing, Recombinant, Mass Spectrometry, Isolation, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Western Blot

    Effects of pH on the rate of inactivation of M. gordonae . Experimental conditions were a temperature of 25°C and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. No, initial number of CFU; N, number of CFU at each time point. Experiments were conducted with different phosphate (0.05 M) buffers within the pH range of 6.0 to 8.0. Slopes were calculated as follows: pH 6, y = 0.11 x ; pH 7, y = 0.09 x ; and pH 8, y = 0.02 x . R 2 , correlation coefficient values.

    Journal: Applied and Environmental Microbiology

    Article Title: Chlorine Disinfection of Atypical Mycobacteria Isolated from a Water Distribution System

    doi: 10.1128/AEM.68.3.1025-1032.2002

    Figure Lengend Snippet: Effects of pH on the rate of inactivation of M. gordonae . Experimental conditions were a temperature of 25°C and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. No, initial number of CFU; N, number of CFU at each time point. Experiments were conducted with different phosphate (0.05 M) buffers within the pH range of 6.0 to 8.0. Slopes were calculated as follows: pH 6, y = 0.11 x ; pH 7, y = 0.09 x ; and pH 8, y = 0.02 x . R 2 , correlation coefficient values.

    Article Snippet: Moreover, when M. gordonae was grown in filtered water supplemented with 10% 7H9 Middlebrook liquid medium, the inactivation rates decreased by a factor 10.

    Techniques: Concentration Assay

    Effects of temperature on chlorine inactivation of M. gordonae . Experimental conditions were pH 7 and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. The chlorine susceptibility of M. gordonae was analyzed at 4, 16, and 25°C. No, initial number of CFU; N, number of CFU at each time point. Symbols: ▪, 4°C ( y = 0.01 x ); ▴, 16°C ( y = 0.02 x ); ⧫, 25°C ( y = 0.09 x ). R 2 , correlation coefficient values.

    Journal: Applied and Environmental Microbiology

    Article Title: Chlorine Disinfection of Atypical Mycobacteria Isolated from a Water Distribution System

    doi: 10.1128/AEM.68.3.1025-1032.2002

    Figure Lengend Snippet: Effects of temperature on chlorine inactivation of M. gordonae . Experimental conditions were pH 7 and an initial chlorine concentration of 0.5 mg/liter. Cells were grown in Middlebrook 7H9-Tween medium. The chlorine susceptibility of M. gordonae was analyzed at 4, 16, and 25°C. No, initial number of CFU; N, number of CFU at each time point. Symbols: ▪, 4°C ( y = 0.01 x ); ▴, 16°C ( y = 0.02 x ); ⧫, 25°C ( y = 0.09 x ). R 2 , correlation coefficient values.

    Article Snippet: Moreover, when M. gordonae was grown in filtered water supplemented with 10% 7H9 Middlebrook liquid medium, the inactivation rates decreased by a factor 10.

    Techniques: Concentration Assay

    Malachite green interferes with postantibiotic recovery of M. smegmatis on solid culture medium. (A and B) M. smegmatis was grown to mid-exponential phase in Middlebrook 7H9 broth and incubation was continued after the addition of 50 μg/ml isoniazid

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Malachite Green Interferes with Postantibiotic Recovery of Mycobacteria

    doi: 10.1128/AAC.00406-12

    Figure Lengend Snippet: Malachite green interferes with postantibiotic recovery of M. smegmatis on solid culture medium. (A and B) M. smegmatis was grown to mid-exponential phase in Middlebrook 7H9 broth and incubation was continued after the addition of 50 μg/ml isoniazid

    Article Snippet: Cells were grown at 37°C to mid-exponential phase in Middlebrook 7H9 liquid culture medium ( ) and diluted 10-fold in fresh 7H9 broth.

    Techniques: Incubation

    Inhibition of postantibiotic recovery by malachite green is specific to antibiotics that target cell wall biogenesis. (A to F) M. smegmatis was grown to mid-exponential phase in Middlebrook 7H9 broth, and incubation was continued for another 24 h after

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Malachite Green Interferes with Postantibiotic Recovery of Mycobacteria

    doi: 10.1128/AAC.00406-12

    Figure Lengend Snippet: Inhibition of postantibiotic recovery by malachite green is specific to antibiotics that target cell wall biogenesis. (A to F) M. smegmatis was grown to mid-exponential phase in Middlebrook 7H9 broth, and incubation was continued for another 24 h after

    Article Snippet: Cells were grown at 37°C to mid-exponential phase in Middlebrook 7H9 liquid culture medium ( ) and diluted 10-fold in fresh 7H9 broth.

    Techniques: Inhibition, Incubation

    Effect of albumin or ROS scavengers on inhibition of postantibiotic recovery by malachite green. (A to C) M. smegmatis was grown to mid-exponential phase in Middlebrook 7H9 broth (A and C) or in 7H9 broth containing 10% OADC (B) and incubation was continued

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Malachite Green Interferes with Postantibiotic Recovery of Mycobacteria

    doi: 10.1128/AAC.00406-12

    Figure Lengend Snippet: Effect of albumin or ROS scavengers on inhibition of postantibiotic recovery by malachite green. (A to C) M. smegmatis was grown to mid-exponential phase in Middlebrook 7H9 broth (A and C) or in 7H9 broth containing 10% OADC (B) and incubation was continued

    Article Snippet: Cells were grown at 37°C to mid-exponential phase in Middlebrook 7H9 liquid culture medium ( ) and diluted 10-fold in fresh 7H9 broth.

    Techniques: Inhibition, Incubation

    Malachite green interferes with postantibiotic recovery of M. tuberculosis on solid culture medium. (A to F) M. tuberculosis was grown to mid-exponential phase in Middlebrook 7H9 broth, and incubation was continued for another 48 h after the addition

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Malachite Green Interferes with Postantibiotic Recovery of Mycobacteria

    doi: 10.1128/AAC.00406-12

    Figure Lengend Snippet: Malachite green interferes with postantibiotic recovery of M. tuberculosis on solid culture medium. (A to F) M. tuberculosis was grown to mid-exponential phase in Middlebrook 7H9 broth, and incubation was continued for another 48 h after the addition

    Article Snippet: Cells were grown at 37°C to mid-exponential phase in Middlebrook 7H9 liquid culture medium ( ) and diluted 10-fold in fresh 7H9 broth.

    Techniques: Incubation

    prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook 7H9 broth with glycerol. The optical density at 600

    Journal: Journal of Bacteriology

    Article Title: The prrAB Two-Component System Is Essential for Mycobacterium tuberculosis Viability and Is Induced under Nitrogen-Limiting Conditions

    doi: 10.1128/JB.06258-11

    Figure Lengend Snippet: prrA and prrB transcription and PrrA levels during in vitro growth. (A) Quantitation of prrA and prrB transcripts during M. tuberculosis H37Rv in vitro exponential growth in supplemented Middlebrook 7H9 broth with glycerol. The optical density at 600

    Article Snippet: M. tuberculosis H37Rv cultures were grown at 37°C in Middlebrook 7H9 basal liquid medium (Difco) supplemented with 10% ADS (0.5% bovine serum albumin fraction V, 0.2% dextrose, 140 mM NaCl) enrichment and 0.05% Tween 80 (herein described as supplemented Middlebrook 7H9) with or without 0.2% glycerol and Hyg (100 μg/ml) when required.

    Techniques: In Vitro, Quantitation Assay

    —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid 7H9 medium supplemented with 0.05% Tween 80, ADC supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).

    Journal: Genome Biology and Evolution

    Article Title: Unexpected Genomic and Phenotypic Diversity of Mycobacterium africanum Lineage 5 Affects Drug Resistance, Protein Secretion, and Immunogenicity

    doi: 10.1093/gbe/evy145

    Figure Lengend Snippet: —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid 7H9 medium supplemented with 0.05% Tween 80, ADC supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).

    Article Snippet: Cultivation of all isolates ( , online) was performed on 7H11 solid medium with OADC supplement (Difco), or in liquid 7H9 medium supplemented with ADC (Difco), 0.05% Tween 80, and 0.2% w/v pyruvate ( ).

    Techniques:

    Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook 7H10 plates and incubated at 37 °C for 21 days.

    Journal: Scientific Reports

    Article Title: Assessing the role of Rv1222 (RseA) as an anti-sigma factor of the Mycobacterium tuberculosis extracytoplasmic sigma factor SigE

    doi: 10.1038/s41598-019-41183-4

    Figure Lengend Snippet: Growth of M. tuberculosis mutant strains in liquid and solid medium. ( A ) Growth curves in liquid medium. Bacteria were grown in 7H9 ADC at 37 °C in rolling bottles; OD 540 was recorded at regular intervals up to 96 hrs; results represent the average of three independent experiments; ( B ) Growth in solid medium. Bacteria were inoculated in Middlebrook 7H10 plates and incubated at 37 °C for 21 days.

    Article Snippet: M. tuberculosis H37Rv was grown in either Middlebrook 7H9 liquid medium or Middlebrook 7H10 solid medium (Difco) supplemented with 0.05% Tween 80 and ADN (2% glucose, 5% bovine serum albumin, 0.85% NaCl).

    Techniques: Mutagenesis, Incubation

    Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in Middlebrook 7H9 medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation

    Journal:

    Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿

    doi: 10.1128/JB.00257-07

    Figure Lengend Snippet: Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in Middlebrook 7H9 medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation

    Article Snippet: M. smegmatis mc2 155 was grown in liquid cultures using Middlebrook 7H9 medium (Difco) supplemented with 0.2% glycerol and 0.05% Tween 80 or minimal Hartmans-de Bont (HB) medium ( ) at 37°C.

    Techniques:

    In vitro growth kinetics of M. tuberculosis Erdman wild type and the five rpf deletion mutant strains in Middlebrook 7H9 medium at 37°C with agitation. The ΔRv1009 strain used in this experiment was mc 2 3126. We also found that strain mc

    Journal: Infection and Immunity

    Article Title: Individual Mycobacterium tuberculosis Resuscitation-Promoting Factor Homologues Are Dispensable for Growth In Vitro and In Vivo

    doi: 10.1128/IAI.72.1.515-526.2004

    Figure Lengend Snippet: In vitro growth kinetics of M. tuberculosis Erdman wild type and the five rpf deletion mutant strains in Middlebrook 7H9 medium at 37°C with agitation. The ΔRv1009 strain used in this experiment was mc 2 3126. We also found that strain mc

    Article Snippet: M. tuberculosis Erdman was grown at 37°C in liquid Middlebrook 7H9 medium supplemented with 0.2% glycerol, 0.05% Tween 80, and 10% OADC enrichment (Becton Dickinson) or on solid Middlebrook 7H10 medium (Difco) supplemented with 0.5% glycerol and 10% OADC.

    Techniques: In Vitro, Mutagenesis