Journal: Molecular Genetics & Genomic Medicine

Article Title: miR‐33a inhibits cell growth in renal cancer by downregulation of MDM4 expression

doi: 10.1002/mgg3.833

Figure Lengend Snippet: Inhibitory effects of miR‐33a on cell proliferation and cell cycle in renal cell cancer (RCC) cell lines. (a) Expression of miR‐33a in Caki‐1 and 786‐O cells after transfection with miR‐33a mimics and miR‐33a inhibitor or NC. NC represents negative control of miRNA. Results were expressed as of three independent experiments, with at least three replicates in each independent experiment, ** p < 0.01 versus NC. (b) CCK‐8 assays indicated that the effects of miR‐33a on the growth of RCC cell lines. Results were expressed as of three independent experiments, with at least three replicates in each independent experiment, * p < 0.05 versus NC. (c, d) The results of flow cytometry showed that upregulation of miR‐33a significantly increased the percentages of cells in the G0/G1 phase, which showed that the upregulation of miR‐33a could suppress RCC cells proliferation. * p < 0.05 versus Control

Article Snippet: Normal primary renal tubular HK‐2 cell lines and RCC cell lines (Caki‐1, ACHN and 786‐O) were purchased from China Center For Type Culture Collection (Wuhan, China).

Techniques: Expressing, Transfection, Negative Control, CCK-8 Assay, Flow Cytometry, Control

Journal: Molecular Genetics & Genomic Medicine

Article Title: miR‐33a inhibits cell growth in renal cancer by downregulation of MDM4 expression

doi: 10.1002/mgg3.833

Figure Lengend Snippet: miR‐33a directly targets Mouse double minute 4 (MDM4). (a) The predicted miR‐33a binding site within MDM4 3′ UTR and miR‐33a mutated version by site mutagenesis; (b) Caki‐1 cells were transfected with reporter constructs containing either wild type (WT) MDM4 , or MDM 4 3′ UTR with mutation (MUT), along with miR‐33a mimics, or negative control, respectively. Relative luciferase activity was measured. (c) qPCR analysis of MDM4 expression in renal cell cancer (RCC) Caki‐1 cells after overexpression or knockdown of miR‐33a . (d, e) Western blotting analysis of MDM4 protein expression in RCC Caki‐1 cells after overexpression or knockdown of miR‐33a . (f) Western blot analysis revealed that transfection of MDM4 siRNA into Caki‐1 cells resulted in decreased MDM4 expression compared to the cells transfected with scrambled siRNA. These effects of siRNA were attenuated by anti‐ miR‐33a inhibitor transfection. NC represents normal control, * p < 0.05, ** p < 0.01 versus NC

Article Snippet: Normal primary renal tubular HK‐2 cell lines and RCC cell lines (Caki‐1, ACHN and 786‐O) were purchased from China Center For Type Culture Collection (Wuhan, China).

Techniques: Binding Assay, Mutagenesis, Transfection, Construct, Negative Control, Luciferase, Activity Assay, Expressing, Over Expression, Knockdown, Western Blot, Control

Journal: Molecular Genetics & Genomic Medicine

Article Title: miR‐33a inhibits cell growth in renal cancer by downregulation of MDM4 expression

doi: 10.1002/mgg3.833

Figure Lengend Snippet: The effect of mouse double minute 4 ( MDM4 ) on cell growth in renal cell cancer (RCC). (a, b, c) RT‐qPCR and western blot analysis of MDM4 expression in RCC cells. (d) Kaplan–Meier analysis of overall survival in 30 RCC patients with low median ( n = 15) and high median ( n = 15) expression levels of MDM4 . (e) CCK‐8 assays indicated that the effects of MDM4 on growth of RCC cell lines. Results were expressed as ± x - s of three independent experiments, with at least three replicates in each independent experiment, * p < 0.05 versus NC. (f) Western blot analysis of p53 expression after miR‐33a mimics and ‐inhibitor transfection in RCC cells

Article Snippet: Normal primary renal tubular HK‐2 cell lines and RCC cell lines (Caki‐1, ACHN and 786‐O) were purchased from China Center For Type Culture Collection (Wuhan, China).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, CCK-8 Assay, Transfection

Journal: Scientific Reports

Article Title: ORM1 promotes tumor progression of kidney renal clear cell carcinoma (KIRC) through CALR-mediated apoptosis

doi: 10.1038/s41598-023-42962-w

Figure Lengend Snippet: ORM1 was essential to cell proliferation. ( a ) The expression of ORM1 protein in A498, 786-O, and Caki-2 cells was much higher than the 293 T cells. ( b ) The gray value analysis of protein in ( a ). ( c ) ORM1 was knocked down in 786-O and Caki-2 cells. ( d ) The gray value analysis of protein in ( c ). ( e ) Cell proliferation of 786-O cells was inhibited in ORM1-KD group compared to NC group. ( f ) Cell proliferation of Caki-2 cells was inhibited in ORM1-KD group compared to NC group. # p < 0.05 showed statistically difference.

Article Snippet: KIRC cell lines including 786-O, A498, and Caki-2 cells were obtained from ICell Bioscience Inc, Shanghai (Shanghai, China).

Techniques: Expressing

Journal: Scientific Reports

Article Title: ORM1 promotes tumor progression of kidney renal clear cell carcinoma (KIRC) through CALR-mediated apoptosis

doi: 10.1038/s41598-023-42962-w

Figure Lengend Snippet: ORM1 was essential to cell migration and invasion. ( a ) Cell migration and invasion was suppressed in ORM1-KD group compared to NC group in 786-O and Caki-2 cells in transwell assay with/without Matrigel. ( b ) The statistical analysis of cells in cell migration in ( a ). ( c ) The statistical analysis of cells in cell invasion in a. # p < 0.05 showed statistically difference.

Article Snippet: KIRC cell lines including 786-O, A498, and Caki-2 cells were obtained from ICell Bioscience Inc, Shanghai (Shanghai, China).

Techniques: Migration, Transwell Assay

Journal: Scientific Reports

Article Title: ORM1 promotes tumor progression of kidney renal clear cell carcinoma (KIRC) through CALR-mediated apoptosis

doi: 10.1038/s41598-023-42962-w

Figure Lengend Snippet: ORM1 enhanced the efficiency of sorafenib in KIRC. ( a ) Sorafenib inhibited cell proliferation in concentration-dependent manner. ( b ) The efficiency of sorafenib was enhanced in ORM1-KD group but relieved in ORM1-OE group in 786-O cells. ( c ) The efficiency of sorafenib was enhanced in ORM1-KD group but relieved in ORM1-OE group in Caki-2 cells. # p < 0.05 showed statistically difference.

Article Snippet: KIRC cell lines including 786-O, A498, and Caki-2 cells were obtained from ICell Bioscience Inc, Shanghai (Shanghai, China).

Techniques: Concentration Assay