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90
ATCC cell density culture precipitated strain a600 viscosity cp biomass g l atcc
Cell Density Culture Precipitated Strain A600 Viscosity Cp Biomass G L Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation caspase-8 antibody - bsa free
Caspase 8 Antibody Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Novus Biologicals mouse cd138
Fig. 4 Necroptosis promotes tumor metastasis by activating cell surface proteases. a, b ELISA of soluble JAM-A, <t>CD138</t> and E-cad in TIF(a) and serum (b) from WT and Mlkl KO MMTV-PyMT mice at 15 weeks (n = 6, each). c, d ELISA of soluble JAM-A, CD138 and E-cad in TIF (c) and serum (d) from MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). e qPCR analysis of IFN-γ mRNA expression in tumor-infiltrating CD8+ T cells of MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each; left panel). Flow cytometry analysis of tumor-infiltrating GZMB+ CD8+ T cells in MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each; right panel). f qPCR analysis of IFN-γ and T-bet mRNA expression in tumor-infiltrating CD4+ T cells of MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). g Flow cytometry analysis of peripheral blood GZMB+ CD8+ T cells and IFN-γ+ CD4+ T cells MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). h Left panel shows the representative images of H&E-stained lung sections from MMTV-PyMT mice treated with vehicle or GW280264X.Scale bar, 2 mm. Right panel shows the quantification of metastatic foci in lungs from MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). Data presented as the mean ± sem and p value was determined by two-tailed t test (a–h)
Mouse Cd138, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse cd138 - by Bioz Stars, 2026-05
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92
Novus Biologicals nbp1 76610
Fig. 4 Necroptosis promotes tumor metastasis by activating cell surface proteases. a, b ELISA of soluble JAM-A, <t>CD138</t> and E-cad in TIF(a) and serum (b) from WT and Mlkl KO MMTV-PyMT mice at 15 weeks (n = 6, each). c, d ELISA of soluble JAM-A, CD138 and E-cad in TIF (c) and serum (d) from MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). e qPCR analysis of IFN-γ mRNA expression in tumor-infiltrating CD8+ T cells of MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each; left panel). Flow cytometry analysis of tumor-infiltrating GZMB+ CD8+ T cells in MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each; right panel). f qPCR analysis of IFN-γ and T-bet mRNA expression in tumor-infiltrating CD4+ T cells of MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). g Flow cytometry analysis of peripheral blood GZMB+ CD8+ T cells and IFN-γ+ CD4+ T cells MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). h Left panel shows the representative images of H&E-stained lung sections from MMTV-PyMT mice treated with vehicle or GW280264X.Scale bar, 2 mm. Right panel shows the quantification of metastatic foci in lungs from MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). Data presented as the mean ± sem and p value was determined by two-tailed t test (a–h)
Nbp1 76610, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nbp1 76610/product/Novus Biologicals
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nbp1 76610 - by Bioz Stars, 2026-05
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Bio-Techne corporation mouse syndecan-1/cd138 elisa kit (colorimetric)
Fig. 4 Necroptosis promotes tumor metastasis by activating cell surface proteases. a, b ELISA of soluble JAM-A, <t>CD138</t> and E-cad in TIF(a) and serum (b) from WT and Mlkl KO MMTV-PyMT mice at 15 weeks (n = 6, each). c, d ELISA of soluble JAM-A, CD138 and E-cad in TIF (c) and serum (d) from MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). e qPCR analysis of IFN-γ mRNA expression in tumor-infiltrating CD8+ T cells of MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each; left panel). Flow cytometry analysis of tumor-infiltrating GZMB+ CD8+ T cells in MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each; right panel). f qPCR analysis of IFN-γ and T-bet mRNA expression in tumor-infiltrating CD4+ T cells of MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). g Flow cytometry analysis of peripheral blood GZMB+ CD8+ T cells and IFN-γ+ CD4+ T cells MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). h Left panel shows the representative images of H&E-stained lung sections from MMTV-PyMT mice treated with vehicle or GW280264X.Scale bar, 2 mm. Right panel shows the quantification of metastatic foci in lungs from MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). Data presented as the mean ± sem and p value was determined by two-tailed t test (a–h)
Mouse Syndecan 1/Cd138 Elisa Kit (Colorimetric), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse syndecan-1/cd138 elisa kit (colorimetric)/product/Bio-Techne corporation
Average 90 stars, based on 1 article reviews
mouse syndecan-1/cd138 elisa kit (colorimetric) - by Bioz Stars, 2026-05
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Standard format: Plasmid sent in bacteria as agar stab
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Fig. 4 Necroptosis promotes tumor metastasis by activating cell surface proteases. a, b ELISA of soluble JAM-A, CD138 and E-cad in TIF(a) and serum (b) from WT and Mlkl KO MMTV-PyMT mice at 15 weeks (n = 6, each). c, d ELISA of soluble JAM-A, CD138 and E-cad in TIF (c) and serum (d) from MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). e qPCR analysis of IFN-γ mRNA expression in tumor-infiltrating CD8+ T cells of MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each; left panel). Flow cytometry analysis of tumor-infiltrating GZMB+ CD8+ T cells in MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each; right panel). f qPCR analysis of IFN-γ and T-bet mRNA expression in tumor-infiltrating CD4+ T cells of MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). g Flow cytometry analysis of peripheral blood GZMB+ CD8+ T cells and IFN-γ+ CD4+ T cells MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). h Left panel shows the representative images of H&E-stained lung sections from MMTV-PyMT mice treated with vehicle or GW280264X.Scale bar, 2 mm. Right panel shows the quantification of metastatic foci in lungs from MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). Data presented as the mean ± sem and p value was determined by two-tailed t test (a–h)

Journal: Breast cancer research : BCR

Article Title: Tumor necroptosis-mediated shedding of cell surface proteins promotes metastasis of breast cancer by suppressing anti-tumor immunity.

doi: 10.1186/s13058-023-01604-9

Figure Lengend Snippet: Fig. 4 Necroptosis promotes tumor metastasis by activating cell surface proteases. a, b ELISA of soluble JAM-A, CD138 and E-cad in TIF(a) and serum (b) from WT and Mlkl KO MMTV-PyMT mice at 15 weeks (n = 6, each). c, d ELISA of soluble JAM-A, CD138 and E-cad in TIF (c) and serum (d) from MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). e qPCR analysis of IFN-γ mRNA expression in tumor-infiltrating CD8+ T cells of MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each; left panel). Flow cytometry analysis of tumor-infiltrating GZMB+ CD8+ T cells in MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each; right panel). f qPCR analysis of IFN-γ and T-bet mRNA expression in tumor-infiltrating CD4+ T cells of MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). g Flow cytometry analysis of peripheral blood GZMB+ CD8+ T cells and IFN-γ+ CD4+ T cells MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). h Left panel shows the representative images of H&E-stained lung sections from MMTV-PyMT mice treated with vehicle or GW280264X.Scale bar, 2 mm. Right panel shows the quantification of metastatic foci in lungs from MMTV-PyMT mice treated with vehicle or GW280264X (n = 5, each). Data presented as the mean ± sem and p value was determined by two-tailed t test (a–h)

Article Snippet: Samples were then analyzed for mouse JAM-A (ab277080, Abcam), mouse CD138 (NBP2-76610, Novus) and mouse E-cadherin (ab197751, Abcam) according to manufacturer’s instruction.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry, Staining, Two Tailed Test