Journal: bioRxiv

Article Title: Synergistic Electroceutical-Glucocorticoid Intervention Mitigates Dexamethasone-Induced Muscle Atrophy in Aging Skeletal Muscle

doi: 10.64898/2026.03.10.709862

Figure Lengend Snippet: (a, b) TA muscle volume measurements from CT images (a) and corresponding quantification (b). (c) Quantification of TA muscle weight. (d) Representative images of CSA of Type IIA muscle fibers in young and aged mice (Blue: Nuclei, Green: Type IIA fibers, Red: laminin) (scale bar: 200 μm). (e-g) Quantification of myofiber CSA (e) comparison between young and aged controls, (f) comparison among young control, DEX-treated, and DEX+ES groups; (g) comparison among aged control, DEX-treated, and DEX+ES groups (n=3). (h-j) Myogenesis-related qPCR array results: (h) young control versus aged control; (i) aged DEX-treated group versus aged control; and (j) aged DEX with electroceutical-treated group versus aged control. ( * P < 0.05; ** P < 0.01; *** P < 0.001 versus young control; # P < 0.05; ## P < 0.01; ### P < 0.001 versus aged control )

Article Snippet: The sections underwent fixation and permeabilization, followed by incubation with antibodies against laminin (1:200 PA1-16730, Abcam, UK) and myosin heavy chain Type IIA (1:200 SC-71, DSHB, IA) for 2 hours.

Techniques: Comparison, Control

Journal: Cilia

Article Title: Abnormal glycosylation in Joubert syndrome type 10.

doi: 10.1186/s13630-017-0048-6

Figure Lengend Snippet: Fig. 1 Clinical and molecular evidence for Joubert syndrome type 10 diagnosis. a Magnetic resonance imaging of the proband, UDP-3331, reveals the hallmark “molar tooth sign” of the superior cerebellar peduncles (arrow). b Sanger sequencing of both the proband, UDP-3331, and his mother, UDP-4786, confirms an OFD1 single-nucleotide deletion (c.2656del) in the proband and maternal inheritance. c Analysis of mRNA transcripts from adult control cells (“WT”), UDP-3331, and UDP-3331 + OFD1 rescue shows reduced mRNA levels in the proband. Error bars represent standard error of four replicates; data are normalized to GAPDH expression and plotted relative to WT OFD1 expression levels. Asterisk indicates p < 0.001 for two- tailed, heteroscedastic Student’s t test. d Western blot analysis of OFD1 levels in UDP-3331, UDP-3331 + OFD1 and adult WT primary fibroblast lines; one representative blot with OFD1 and α-tubulin is shown in the top panels; average of 3 replicate blots with independent lysates is shown in the lower panel. OFD1 levels are normalized to α-tubulin and plotted relative to WT OFD1 protein levels

Article Snippet: OFD1 cloning Wild-type (WT) cDNA expression constructs were made by PCR amplification of the OFD1 cDNA sequence from a sequence-verified TrueORF Gold clone (RC220154, Origene, Rockville, Maryland).

Techniques: Biomarker Discovery, Magnetic Resonance Imaging, Sequencing, Control, Expressing, Two Tailed Test, Western Blot

Journal: Cilia

Article Title: Abnormal glycosylation in Joubert syndrome type 10.

doi: 10.1186/s13630-017-0048-6

Figure Lengend Snippet: Fig. 2 Cellular morphology and analysis of JBTS10 skin fibroblasts. a Co-localization analysis of OFD1 (magenta) signals in proband, proband rescued, and adult WT cells with ɣ-tubulin (red) and PCM1 (green). Inset higher magnification of the region outlined with dotted lines. b Immunofluo‑ rescence staining for OFD1 (green) and α-tubulin (red) in fibroblasts of the proband (UDP-3331), proband rescued (UDP-3331 + OFD1), and adult WT control. c Comparison of endoplasmic reticulum structure (KDEL, green) in UDP-3331 and WT cells. d Comparison of the cis- (GM-130, green) and trans- (TGN-46, red) Golgi in UDP-3331 and WT cells. DNA was stained with Hoechst 33,342 (blue). All scale bars represent 20 µm

Article Snippet: OFD1 cloning Wild-type (WT) cDNA expression constructs were made by PCR amplification of the OFD1 cDNA sequence from a sequence-verified TrueORF Gold clone (RC220154, Origene, Rockville, Maryland).

Techniques: Staining, Control, Comparison

Journal: Cilia

Article Title: Abnormal glycosylation in Joubert syndrome type 10.

doi: 10.1186/s13630-017-0048-6

Figure Lengend Snippet: Fig. 3 Analysis of ciliogenesis in JBTS10 and control skin fibro‑ blasts. a The percentage of ciliated cells following 48 h of serum starvation in UDP-3331, UDP-3331 + OFD1 rescue, adult WT, adult WT + OFD1 overexpression, UW172-4, and fetal WT control cells. Error bars represent the 95% confidence interval for the average of three independent experiments. Asterisks indicate p < 0.001, Student’s t test. b Representative images of ciliated cells for the indicated cell lines. Acetylated tubulin (red) and ɣ-tubulin (green) mark the cilia and basal body, respectively. DNA was stained with DAPI (blue). Scale bars 10 µm

Article Snippet: OFD1 cloning Wild-type (WT) cDNA expression constructs were made by PCR amplification of the OFD1 cDNA sequence from a sequence-verified TrueORF Gold clone (RC220154, Origene, Rockville, Maryland).

Techniques: Control, Over Expression, Staining

Journal: Cilia

Article Title: Abnormal glycosylation in Joubert syndrome type 10.

doi: 10.1186/s13630-017-0048-6

Figure Lengend Snippet: Fig. 5 Nucleotide sugar analysis in JBTS10 and control skin fibroblasts. a Representative HPAEC trace for UDP-3331, UDP-3331 + OFD1 rescue, adult WT, adult WT + OFD1 overexpression, pediatric male control cells, and 500 nM standard CMP-sialic acid. b Quantification of CMP-sialic acid levels in affected and control cells (in μM) (single asterisks indicate p < 0.005 in an unpaired, heteroscedastic t test). Error bars represent standard error

Article Snippet: OFD1 cloning Wild-type (WT) cDNA expression constructs were made by PCR amplification of the OFD1 cDNA sequence from a sequence-verified TrueORF Gold clone (RC220154, Origene, Rockville, Maryland).

Techniques: Control, Over Expression