Journal: Cellular Oncology (Dordrecht, Netherlands)

Article Title: TRIM47-facilitated PLK1 stabilization promotes the proliferation of liver cancer cells

doi: 10.1007/s13402-025-01130-0

Figure Lengend Snippet: The NF-κB and MAPK pathways are involved in TRIM47-induced cell proliferation in LC. A. The total and phosphorylated levels of key proteins in the NF-κB, PI3K/AKT and MAPK pathways were detected in LC cells after TRIM47 silencing or overexpression. B. Protein quantitative analysis of A with 3 independent immunoblotting results. C. Western blotting was used to detect the activation of the above signaling pathways in Hep3B tumor tissues. The right panel shows the corresponding protein quantitative analysis. D. The total amount and phosphorylation levels of key proteins in the above signaling pathways were detected by western blotting after volasertib treatment. E. Control and TRIM47-overexpressed Huh7 stable cell lines were treated with AZD6244 (5 µM), AZD5363 (10 µM) or BAY-11-7082 (10 µM) for 48 h and then detected via a CCK-8, respectively. The OD450 value was detected and analyzed after treatment. F. Representative images of the colony formation assay in Huh7 cells after treatment with the above inhibitors. G. Statistical analysis of the number of cell clones with three replicates. The data are shown as the means ± SD. n.s. P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Inhibitors Volasertib, BAY11-7082, Capivasertib (AZD5363), and Selumetinib (AZD6244) were purchased from Topscience (Shanghai, China).

Techniques: Over Expression, Western Blot, Activation Assay, Protein-Protein interactions, Phospho-proteomics, Control, Stable Transfection, CCK-8 Assay, Colony Assay, Clone Assay