Journal: JCI Insight

Article Title: Increased hepatic glucose production with lower oxidative metabolism in the growth-restricted fetus

doi: 10.1172/jci.insight.176497

Figure Lengend Snippet: ( A ) Protein expression in CON and FGR livers for phosphorylated (ph-) and total protein abundance of AMPK (T172), JNK (T183/Y185), and FOXO1 (T24). Ratios of phosphorylated to total protein were calculated. ( B ) Representative Western blot images, with molecular weights of protein bands indicated. Actin shown for equality of loading. ( C ) Total abundance of NRF-2. ( D ) Hepatic expression of genes associated with nutrient and stress signaling. ( E ) Hepatic TBARS content. ( F ) Liver tissue metabolite abundance ratios for AMP/ATP, NADH/NAD+, NADPH/NADP+, and lactate/pyruvate (Lac:Pyr). ( G ) Blood lactate/pyruvate ratio measured across the fetal hepatic circulation with input (h in ) and output (h out ) shown. Means ± SEM are shown ( n = 6 CON, 9–10 FGR, except panel E with n = 14 CON, 21 FGR). Results were analyzed by Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001. When P > 0.05, the value is shown. Data points on graphs or blot lanes representing samples from FGR fetuses with moderate (pink) or severe (red) growth restriction are indicated. Weight-threshold differences comparing moderate (light pink) versus severe (dark red) FGR were analyzed by 2-tailed Student’s t test and are indicated by a vertical line.

Article Snippet: TBARS content (700870, Cayman Chemical) was measured as described previously ( ).

Techniques: Expressing, Quantitative Proteomics, Western Blot

Journal: Translational Animal Science

Article Title: Effect of supplementing a Saccharomyces cerevisiae fermentation product during a preconditioning period prior to transit on receiving period performance, nutrient digestibility, and antioxidant defense by beef steers

doi: 10.1093/tas/txz140

Figure Lengend Snippet: Effect of day on plasma malondialdehyde concentrations of beef steers in relation to a 19-h transit event; initial blood samples were collected immediately prior to transit on day −1. Based on repeated measures analysis, bars with unlike superscripts indicate a difference ( P ≤ 0.05) between days (day P < 0.01; treatment × day P ≥ 0.94).

Article Snippet: Plasma was removed, aliquoted, and stored at −80°C until analysis of malondialdehyde (MDA) concentrations (#700870, Cayman Chemical, Ann Arbor, MI); inter- and intra-assay CV were 7.7% and 6.4%, respectively.

Techniques: Clinical Proteomics