66451 Search Results


irf4  (Proteintech)
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Proteintech irf4
Baicalin inhibits LPS‐induced M1 macrophage polarization in vitro. A) Macrophages in CON, LPS, and BL + LPS group. B) mRNA expression of STAT1 , STAT2 , STAT3 , and TLR2 in CON, LPS, and BL + LPS group ( n = 4). C) mRNA expression of TLR4 , <t>IRF4</t> , IRF7 , IRF8 , IRF9 , CD86 , IL‐1β , NLRP3 , IL‐12 , and MMP9 in CON, LPS, and BL + LPS group ( n = 4). D) Immunofluorescence analyses of CD86, IL‐1β, NLRP3, and TLR4 protein expression in RAW264.7 cells. Nuclei are counterstained with DAPI (blue). (E) Mean gray value of CD86, IL‐1β, NLRP3, and TLR4 in immunofluorescence analyses ( n = 3).
Irf4, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Baicalin inhibits LPS‐induced M1 macrophage polarization in vitro. A) Macrophages in CON, LPS, and BL + LPS group. B) mRNA expression of STAT1 , STAT2 , STAT3 , and TLR2 in CON, LPS, and BL + LPS group ( n = 4). C) mRNA expression of TLR4 , IRF4 , IRF7 , IRF8 , IRF9 , CD86 , IL‐1β , NLRP3 , IL‐12 , and MMP9 in CON, LPS, and BL + LPS group ( n = 4). D) Immunofluorescence analyses of CD86, IL‐1β, NLRP3, and TLR4 protein expression in RAW264.7 cells. Nuclei are counterstained with DAPI (blue). (E) Mean gray value of CD86, IL‐1β, NLRP3, and TLR4 in immunofluorescence analyses ( n = 3).

Journal: Advanced Science

Article Title: Mechanisms of Baicalin Alleviates Intestinal Inflammation: Role of M1 Macrophage Polarization and Lactobacillus amylovorus

doi: 10.1002/advs.202415948

Figure Lengend Snippet: Baicalin inhibits LPS‐induced M1 macrophage polarization in vitro. A) Macrophages in CON, LPS, and BL + LPS group. B) mRNA expression of STAT1 , STAT2 , STAT3 , and TLR2 in CON, LPS, and BL + LPS group ( n = 4). C) mRNA expression of TLR4 , IRF4 , IRF7 , IRF8 , IRF9 , CD86 , IL‐1β , NLRP3 , IL‐12 , and MMP9 in CON, LPS, and BL + LPS group ( n = 4). D) Immunofluorescence analyses of CD86, IL‐1β, NLRP3, and TLR4 protein expression in RAW264.7 cells. Nuclei are counterstained with DAPI (blue). (E) Mean gray value of CD86, IL‐1β, NLRP3, and TLR4 in immunofluorescence analyses ( n = 3).

Article Snippet: The primary antibodies used were obtained from the following suppliers: anti‐ACTB (Sangon Biotech, #D110001), NLRP3 (Beyotime, #AF2155), IL‐1β (Bioss, #bs‐25615R), Occludin (Sangon Biotech, #D110001), CD163 (Sangon Biotech, #D260965), STAT2 (Proteintech, #16674‐1‐AP), TLR4 (Beyotime, #AF8187), Foxp3 (Beyotime, #AF6927), RORγt (Bioss, #bs‐10647R), IL‐17F (Bioss, #bs‐7333R), CTLA4 (ABclonal, #A13966), IRF4 (Proteintech, #66451‐1‐Ig), IRF7 (Proteintech, #22392‐1‐AP), and TLR9 (Beyotime, #AF8193).

Techniques: In Vitro, Expressing, Immunofluorescence

Lactobacillus amylovorus SKLAN202301ZF regulates macrophage polarization and T cell differentiation. A) mRNA expression of STAT1 , STAT2 , STAT3 , TLR2 , TLR3 , TLR4 , IRF7 , and IRF8 ( n = 12). B) mRNA expression of IRF9 , PIAS1 , PIAS3 , CD226 , Unc93b1 , RORγt , CD86 , and CD163 ( n = 12). C) mRNA expression of NLRP3 , IL‐1β , IL‐17A , IL‐17F , IL‐18 , CCL17 , MMP9 ( n = 12). D) Western blotting was conducted to measure the expression of CTLA4, IRF4, IRF7, TLR9, FOXP3, RORγt, IL‐1β, NLRP3, and ACTB in the colon, E) and the ratio of cleaved to full‐length forms for these proteins was calculated ( n = 4). ACTB was used as a loading control.

Journal: Advanced Science

Article Title: Mechanisms of Baicalin Alleviates Intestinal Inflammation: Role of M1 Macrophage Polarization and Lactobacillus amylovorus

doi: 10.1002/advs.202415948

Figure Lengend Snippet: Lactobacillus amylovorus SKLAN202301ZF regulates macrophage polarization and T cell differentiation. A) mRNA expression of STAT1 , STAT2 , STAT3 , TLR2 , TLR3 , TLR4 , IRF7 , and IRF8 ( n = 12). B) mRNA expression of IRF9 , PIAS1 , PIAS3 , CD226 , Unc93b1 , RORγt , CD86 , and CD163 ( n = 12). C) mRNA expression of NLRP3 , IL‐1β , IL‐17A , IL‐17F , IL‐18 , CCL17 , MMP9 ( n = 12). D) Western blotting was conducted to measure the expression of CTLA4, IRF4, IRF7, TLR9, FOXP3, RORγt, IL‐1β, NLRP3, and ACTB in the colon, E) and the ratio of cleaved to full‐length forms for these proteins was calculated ( n = 4). ACTB was used as a loading control.

Article Snippet: The primary antibodies used were obtained from the following suppliers: anti‐ACTB (Sangon Biotech, #D110001), NLRP3 (Beyotime, #AF2155), IL‐1β (Bioss, #bs‐25615R), Occludin (Sangon Biotech, #D110001), CD163 (Sangon Biotech, #D260965), STAT2 (Proteintech, #16674‐1‐AP), TLR4 (Beyotime, #AF8187), Foxp3 (Beyotime, #AF6927), RORγt (Bioss, #bs‐10647R), IL‐17F (Bioss, #bs‐7333R), CTLA4 (ABclonal, #A13966), IRF4 (Proteintech, #66451‐1‐Ig), IRF7 (Proteintech, #22392‐1‐AP), and TLR9 (Beyotime, #AF8193).

Techniques: Cell Differentiation, Expressing, Western Blot, Control