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Journal: Gut Microbes
Article Title: Gut microbial ammonia as a mediator of PFOS neurotoxicity and its remediation by the flavonoid Icaritin
doi: 10.1080/19490976.2026.2620125
Figure Lengend Snippet: ICT alleviates PFOS-induced activation of hippocampal astrocytes and Aβ pathology, neuroinflammation, and oxidative stress. (A) Representative H&E staining of the hippocampal dentate gyrus (DG) region showing neuronal morphology. Scale bars: 500 μm (upper panel, overview), 50 μm (lower panel, detailed view). (B) Dual immunofluorescence staining of GFAP (green, astrocyte marker) and Aβ (red) in the hippocampal DG region. (C) Quantitative analysis of GFAP fluorescence intensity ( n = 5). (D) Quantitative analysis of Aβ fluorescence intensity ( n = 5). (E) Triple-label immunofluorescence staining of GFAP (green), C3 (red) and S100A10 (purple) in the hippocampal DG region. (F) Quantitative analysis of C3 + GFAP + cells (%) ( n = 5). (G) Quantitative analysis of S100A10 + GFAP + cells (%) ( n = 5). (H−J) Hippocampal levels of pro-inflammatory cytokines: (H) IL-1β ( n = 6), (I) IL-6 ( n = 6), and (J) TNF- α ( n = 6), measured by ELIZA. (K) Reactive oxygen species (ROS) levels in hippocampal tissues ( n = 6). (L) Malondialdehyde (MDA) content, a lipid peroxidation marker ( n = 6). (M−O) Activities of antioxidant enzymes: (M) catalase (CAT, n = 6), (N) superoxide dismutase (SOD, n = 6), and (O) glutathione peroxidase (GSH-Px, n = 6) in hippocampal tissues. The data were presented as the mean ± SEM. ** P < 0.01 vs. Control group; # P < 0.05, ## P < 0.01 vs. PFOS group.
Article Snippet: GFAP (16825-1-AP), C3 (21337-1-AP),
Techniques: Activation Assay, Staining, Immunofluorescence, Marker, Fluorescence, Control