Journal: Scientific Reports

Article Title: Tuning IL-2 signaling by ADP-ribosylation of CD25

doi: 10.1038/srep08959

Figure Lengend Snippet: Splenocytes from DEREG mice were incubated in the absence or presence of the indicated concentrations of NAD + before staining with fluorochrome-conjugated mAbs directed against CD4 and CD25 (mAb PC61 or mAb 7D4). Gating was performed on CD4 + cells. (a) Representative dot plots of cells incubated in the absence or presence of 12 μM NAD + . (b) Percentages of CD4 + GFP + cells staining with anti-CD25 mAbs plotted as a function of the concentration of added NAD + . Results are representative of two independent experiments.

Article Snippet: Alternatively, after erythrocyte lysis with Ack lysis buffer (Bio Whittaker) and depletion of B-cells with Dynabead-conjugated sheep anti-mouse IgG (Invitrogen), Tregs were positively selected by magnetic cell sorting using PE conjugated anti-CD25 (7D4) and magnetic bead-conjugated anti-PE antibodies according to the manufacturer's instructions (Miltenyi Biotec).

Techniques: Incubation, Staining, Concentration Assay

Journal: RSC Advances

Article Title: Binder-free rGO–Si composite anodes with controlled silicon content and composition-dependent electrochemical performance

doi: 10.1039/d6ra01135g

Figure Lengend Snippet: (a) FTIR spectra of Si and rGO–Si; (b) Raman spectra of Si, GO and rGO–Si material.

Article Snippet: In addition, Fourier transform infrared (FTIR) spectroscopy (Jasco-4600) was used to analyze surface functional groups, providing complementary information on chemical bonding within the composite.

Techniques: