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Image Search Results
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Cell density regulates neutrophil IL-8 synthesis: role of IL-1 receptor antagonist and soluble TNF receptors.
doi: 10.4049/jimmunol.166.10.6287
Figure Lengend Snippet: FIGURE 5. Accumulation of IL-1RA and sTNFRs in the supernatants of LPS-treated neutrophils upon increase in cell density. Increasing con- centrations of neutrophils (10 3 106 PMN/ml to 60 3 106 PMN/ml) were challenged with LPS (10 ng/ml). At indicated time points, reactions were stopped, and cell supernatants were collected. IL-1RA (A), sTNFRI (B), and sTNFRII (C) are given in ng/ml. Means 6 SEM of at least four in- dependent experiments are shown. Values marked differ significantly from corresponding values with 10 3 106 PMN/ml.
Article Snippet: Neutralizing Abs targeting IL-1RA (AF-280-NA) and
Techniques:
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Cell density regulates neutrophil IL-8 synthesis: role of IL-1 receptor antagonist and soluble TNF receptors.
doi: 10.4049/jimmunol.166.10.6287
Figure Lengend Snippet: FIGURE 6. Effect of neutralization of IL-1RA and sTNFRs on the down-regulation of LPS-induced neutrophil IL-8 generation upon increase in cell density. Increasing concentrations of neutrophils (10 3 106 PMN/ml to 60 3 106 PMN/ml) were challenged with LPS (10 ng/ml) in the absence or presence of neutralizing Abs targeting IL-1RA, sTNFRI, or sTNFRII. All Abs were used at 20 mg/ml. Reactions were stopped after 16 h, and cell supernatants were collected. IL-8 is given in pg/ml. Means 6 SEM of at least four independent experiments are shown. Values marked differ sig- nificantly from corresponding values without neutralizing Abs.
Article Snippet: Neutralizing Abs targeting IL-1RA (AF-280-NA) and
Techniques: Neutralization
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Cell density regulates neutrophil IL-8 synthesis: role of IL-1 receptor antagonist and soluble TNF receptors.
doi: 10.4049/jimmunol.166.10.6287
Figure Lengend Snippet: FIGURE 7. Effect of exogenous IL-1RA and sTNFRs on LPS-induced IL-8 release from neutrophils incubated at constant cell density. Constant concentrations of neutrophils (10 3 106 PMN/ml) were challenged with LPS (10 ng/ml) in the absence or presence of exogenous IL-1RA, sTNFRs, or a combination of IL-1RA and sTNFRs. IL-1RA was applied at 40 ng/ml, while each sTNFR was given at 20 ng/ml. Reactions were stopped after 16 h, and cell supernatants were collected. IL-8 is given in pg/ml. Means 6 SEM of at least three independent experiments are shown. Values marked differ significantly from mono-LPS-stimulated PMN.
Article Snippet: Neutralizing Abs targeting IL-1RA (AF-280-NA) and
Techniques: Incubation