Journal: Antibiotics

Article Title: Synthesis and Evaluation of the Antibacterial Activities of 13-Substituted Berberine Derivatives

doi: 10.3390/antibiotics9070381

Figure Lengend Snippet: Evaluation of the spectrum of antimicrobial activity of the most active berberine derivatives. Antibacterial and antifungal activities of the most active berberine derivatives identified in Table 1 were further tested using a large panel of microbial strains as explained in the Materials and Methods section. Values in the table correspond to Minimal Inhibitory Concentration (MIC) expressed in µM.

Article Snippet: Environmental and pathogenic Gram-positive bacterial strains used were: Arthrobacter gandavensis (DSM 2447), Bacillus subtilis (ATCC 6633), nisin-resistant B. subtilis (DSMZ 347), Clostridium perfringens (ATCC 13124), Clostridium difficile (DSM 1296), vancomycin-resistant Enterococcus faecalis (DSMZ 13591), Lactococcus lactis (DSM 20481), Staphylococcus aureus (ATCC 6538P), methicillin-resistant S. aureus strain MRSA USA300 (ATCC BAA-1717 USA 300 CA-MRSA) and Streptococcus pyogenes (DSM 20565).

Techniques: Activity Assay, Concentration Assay

Journal: The Journal of Heart and Lung Transplantation

Article Title: Respiratory viral infection in lung transplantation induces exosomes that trigger chronic rejection

doi: 10.1016/j.healun.2019.12.009

Figure Lengend Snippet: Exosomes containing 20S proteasome core in LTxRs with RVI and stable LTxRs. Circulatory exosomes isolated from LTxRs with RVI ( n = 5) and stable LTxRs ( n = 4) were used to detect the presence of 20S proteasome subunit α3 using immunoblot. ( A ) The exosomes isolated from patients with RVI showed a significant increase in 20S proteasome compared with exosomes from stable LTxRs (Mean optical density: 1.74 ± 0.6 vs 0.37 ± 0.35, p = 0.0317 ). Alix served as loading control and exosome-specific marker. ( B ) Graphical representation shows optical intensity of 20S proteasome α3 subunit abundance in LTxRs with viral infection and stable LTxRs. The presence of 20S proteasome was compared between stable LTxRs and LTxRs with RVI using Student's t -test. LTxR, lung transplant recipient; RVI, respiratory viral infection.

Article Snippet: 20S proteasome subunit α3 (sc-58414, Santa Cruz Biotechnology), rhinovirus VP3 (MA5-18249, Thermo Fisher Scientific, Waltham, MA), coronavirus (NB100-64754, Novus Biologicals, Littleton, CO), and RSV glycoprotein G (7950-0980, Bio-Rad Laboratories, Hercules, CA) were used as primary Abs; secondary Abs conjugated with horseradish peroxidase (HRP) were used specific to primary Ab.

Techniques: Isolation, Western Blot, Control, Marker, Infection