6249 Search Results


95
ATCC streptococcus sp strain atcc 6249
Streptococcus Sp Strain Atcc 6249, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
Chem Impex International methyl 4 chloropyridine
Non-enzymatic reactivity of selected electrophiles with thiols. A. Reactivity is estimated by incubation of 1:1 glutahione (GSH):electrophile (1 mM each) for 45 min at a pH of 7.5, 25 °C and quantification of remaining thiol. Compound numbers in this figure correspond to those depicted in Figure 1B. B. Structures of electrophiles tested include 1 (4-chloropyridine), 2 (ampicillin), 3 (styrene oxide), 4 (acrylamide), 5 (iodoacetate), 6 <t>(N-methyl-4-chloropyridine),</t> 7 (iodoacetamide), 8 (phenyl vinylsulfonate), 9 (N-ethylmaleimide). C. The second order rate constants for reaction of thiophenol with acrylamide (○), 4-chloropyridine (□), and N-methyl-4-chloropyridine (■) are determined by plotting kobs values versus the concentration of electrophile and linear fitting gives (2.5 ± 0.1) × 10−3 M−1s−1, (1.2 ± 0.2) × 10−2 M−1s−1, and 54 ± 7 M−1s−1, respectively. Error bars for panels A and C are the standard deviation from three replicate experiments.
Methyl 4 Chloropyridine, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Merck & Co dmem 6249
Non-enzymatic reactivity of selected electrophiles with thiols. A. Reactivity is estimated by incubation of 1:1 glutahione (GSH):electrophile (1 mM each) for 45 min at a pH of 7.5, 25 °C and quantification of remaining thiol. Compound numbers in this figure correspond to those depicted in Figure 1B. B. Structures of electrophiles tested include 1 (4-chloropyridine), 2 (ampicillin), 3 (styrene oxide), 4 (acrylamide), 5 (iodoacetate), 6 <t>(N-methyl-4-chloropyridine),</t> 7 (iodoacetamide), 8 (phenyl vinylsulfonate), 9 (N-ethylmaleimide). C. The second order rate constants for reaction of thiophenol with acrylamide (○), 4-chloropyridine (□), and N-methyl-4-chloropyridine (■) are determined by plotting kobs values versus the concentration of electrophile and linear fitting gives (2.5 ± 0.1) × 10−3 M−1s−1, (1.2 ± 0.2) × 10−2 M−1s−1, and 54 ± 7 M−1s−1, respectively. Error bars for panels A and C are the standard deviation from three replicate experiments.
Dmem 6249, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
EPIC Systems Corporation revenue cycle management solutions vendors n 6249 other epic systems corporation
Non-enzymatic reactivity of selected electrophiles with thiols. A. Reactivity is estimated by incubation of 1:1 glutahione (GSH):electrophile (1 mM each) for 45 min at a pH of 7.5, 25 °C and quantification of remaining thiol. Compound numbers in this figure correspond to those depicted in Figure 1B. B. Structures of electrophiles tested include 1 (4-chloropyridine), 2 (ampicillin), 3 (styrene oxide), 4 (acrylamide), 5 (iodoacetate), 6 <t>(N-methyl-4-chloropyridine),</t> 7 (iodoacetamide), 8 (phenyl vinylsulfonate), 9 (N-ethylmaleimide). C. The second order rate constants for reaction of thiophenol with acrylamide (○), 4-chloropyridine (□), and N-methyl-4-chloropyridine (■) are determined by plotting kobs values versus the concentration of electrophile and linear fitting gives (2.5 ± 0.1) × 10−3 M−1s−1, (1.2 ± 0.2) × 10−2 M−1s−1, and 54 ± 7 M−1s−1, respectively. Error bars for panels A and C are the standard deviation from three replicate experiments.
Revenue Cycle Management Solutions Vendors N 6249 Other Epic Systems Corporation, supplied by EPIC Systems Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Yusheng Pharmaceutical Co Ltd 6249 85 ybp
Non-enzymatic reactivity of selected electrophiles with thiols. A. Reactivity is estimated by incubation of 1:1 glutahione (GSH):electrophile (1 mM each) for 45 min at a pH of 7.5, 25 °C and quantification of remaining thiol. Compound numbers in this figure correspond to those depicted in Figure 1B. B. Structures of electrophiles tested include 1 (4-chloropyridine), 2 (ampicillin), 3 (styrene oxide), 4 (acrylamide), 5 (iodoacetate), 6 <t>(N-methyl-4-chloropyridine),</t> 7 (iodoacetamide), 8 (phenyl vinylsulfonate), 9 (N-ethylmaleimide). C. The second order rate constants for reaction of thiophenol with acrylamide (○), 4-chloropyridine (□), and N-methyl-4-chloropyridine (■) are determined by plotting kobs values versus the concentration of electrophile and linear fitting gives (2.5 ± 0.1) × 10−3 M−1s−1, (1.2 ± 0.2) × 10−2 M−1s−1, and 54 ± 7 M−1s−1, respectively. Error bars for panels A and C are the standard deviation from three replicate experiments.
6249 85 Ybp, supplied by Yusheng Pharmaceutical Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Non-enzymatic reactivity of selected electrophiles with thiols. A. Reactivity is estimated by incubation of 1:1 glutahione (GSH):electrophile (1 mM each) for 45 min at a pH of 7.5, 25 °C and quantification of remaining thiol. Compound numbers in this figure correspond to those depicted in Figure 1B. B. Structures of electrophiles tested include 1 (4-chloropyridine), 2 (ampicillin), 3 (styrene oxide), 4 (acrylamide), 5 (iodoacetate), 6 (N-methyl-4-chloropyridine), 7 (iodoacetamide), 8 (phenyl vinylsulfonate), 9 (N-ethylmaleimide). C. The second order rate constants for reaction of thiophenol with acrylamide (○), 4-chloropyridine (□), and N-methyl-4-chloropyridine (■) are determined by plotting kobs values versus the concentration of electrophile and linear fitting gives (2.5 ± 0.1) × 10−3 M−1s−1, (1.2 ± 0.2) × 10−2 M−1s−1, and 54 ± 7 M−1s−1, respectively. Error bars for panels A and C are the standard deviation from three replicate experiments.

Journal: Chembiochem : a European journal of chemical biology

Article Title: Selective Covalent Protein Modification by 4-Halopyridines Through Catalysis

doi: 10.1002/cbic.201700104

Figure Lengend Snippet: Non-enzymatic reactivity of selected electrophiles with thiols. A. Reactivity is estimated by incubation of 1:1 glutahione (GSH):electrophile (1 mM each) for 45 min at a pH of 7.5, 25 °C and quantification of remaining thiol. Compound numbers in this figure correspond to those depicted in Figure 1B. B. Structures of electrophiles tested include 1 (4-chloropyridine), 2 (ampicillin), 3 (styrene oxide), 4 (acrylamide), 5 (iodoacetate), 6 (N-methyl-4-chloropyridine), 7 (iodoacetamide), 8 (phenyl vinylsulfonate), 9 (N-ethylmaleimide). C. The second order rate constants for reaction of thiophenol with acrylamide (○), 4-chloropyridine (□), and N-methyl-4-chloropyridine (■) are determined by plotting kobs values versus the concentration of electrophile and linear fitting gives (2.5 ± 0.1) × 10−3 M−1s−1, (1.2 ± 0.2) × 10−2 M−1s−1, and 54 ± 7 M−1s−1, respectively. Error bars for panels A and C are the standard deviation from three replicate experiments.

Article Snippet: [ 16 ] 2-Methyl-4-chloropyridine, 3-amino-4-bromopyridine, and 2-amino-4-bromopyridine were from Chem-Impex International, Inc. (Wood Dale, IL).

Techniques: Incubation, Concentration Assay, Standard Deviation

Selectivity of Halopyridines In Labeling E. coli Cell Lysates. A. Western blot with red indicating pre-labeled molecular weight markers and green indicating biotin/probe-labeled proteins. Lanes left to right are: molecular weight markers (bottom to top: 10, 15, 20, 25, 37, 50, 75, 100, 150, (250 only in B) kDa), untreated lysate, lysate heated to 95 °C then cooled and treated with the alkyne-tagged 4-chloropyridine probe shown, unheated lysate treated with the same probe, unheated lysate treated with the alkyne-tagged N-methyl-4-chloropyridine probe shown, lysate heated to 95 °C then cooled and treated with the N-methylated probe shown above. B. Coomassie-stained SDS-PAGE gel run in parallel with that used to create the Western blot in A, with lanes corresponding to those described above.

Journal: Chembiochem : a European journal of chemical biology

Article Title: Selective Covalent Protein Modification by 4-Halopyridines Through Catalysis

doi: 10.1002/cbic.201700104

Figure Lengend Snippet: Selectivity of Halopyridines In Labeling E. coli Cell Lysates. A. Western blot with red indicating pre-labeled molecular weight markers and green indicating biotin/probe-labeled proteins. Lanes left to right are: molecular weight markers (bottom to top: 10, 15, 20, 25, 37, 50, 75, 100, 150, (250 only in B) kDa), untreated lysate, lysate heated to 95 °C then cooled and treated with the alkyne-tagged 4-chloropyridine probe shown, unheated lysate treated with the same probe, unheated lysate treated with the alkyne-tagged N-methyl-4-chloropyridine probe shown, lysate heated to 95 °C then cooled and treated with the N-methylated probe shown above. B. Coomassie-stained SDS-PAGE gel run in parallel with that used to create the Western blot in A, with lanes corresponding to those described above.

Article Snippet: [ 16 ] 2-Methyl-4-chloropyridine, 3-amino-4-bromopyridine, and 2-amino-4-bromopyridine were from Chem-Impex International, Inc. (Wood Dale, IL).

Techniques: Labeling, Western Blot, Molecular Weight, Methylation, Staining, SDS Page