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Addgene inc
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BPS Bioscience
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ATCC
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Bio-Techne corporation
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Santa Cruz Biotechnology
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Image Search Results
Journal: Cell
Article Title: Secreted gelsolin inhibits DNGR-1-dependent cross-presentation and cancer immunity
doi: 10.1016/j.cell.2021.05.021
Figure Lengend Snippet:
Article Snippet:
Techniques: Enzyme-linked Immunosorbent Assay, shRNA, Recombinant, Staining, SYBR Green Assay, Cell Isolation, Expressing, Clone Assay, Plasmid Preparation, Software
Journal: Cancer Immunology, Immunotherapy : CII
Article Title: Efficacy and mechanism of the anti-CD38 monoclonal antibody Daratumumab against primary effusion lymphoma
doi: 10.1007/s00262-021-03054-8
Figure Lengend Snippet: DARA-mediated ADCC based on CD16 expressing NK cells. A BCBL-1, GTO, and TY-1 cells were incubated with various concentrations of DARA, and co-cultured with ADCC-indicating Jurkat cells (E:T ratio = 1:5) for 18 h. Luciferase activity was measured. B, C, D, E) CFSE-labeled BCBL-1 cells were treated with IgG1 or DARA, co-cultured with CD16 + NK (N6) or CD16- NK (KHYG-1) cell lines at different E:T ratio for 4 h, and analyzed for dead BCBL-1 (CFSE+Ghost Dye+) cells by flow cytometry. F Up-regulation of CD107a after DARA treatment was analyzed by flow cytometry. G Percentages of CD56+CD107a+ cells after stimulation with DARA (n = 3). Data are presented as mean values ± standard error (SE). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 significant levels
Article Snippet: Recombinant Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and
Techniques: Expressing, Incubation, Cell Culture, Luciferase, Activity Assay, Labeling, Flow Cytometry