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Image Search Results
Journal: Nature microbiology
Article Title: Genetic basis for the cooperative bioactivation of plant lignans by Eggerthella lenta and other human gut bacteria
doi: 10.1038/s41564-019-0596-1
Figure Lengend Snippet: a , Mouse bacterial colonization levels were assessed by qPCR. Genome equivalents not detected or below the limit of quantitation (3×10 2 genome equivalents) are represented as NQ (not quantifiable). Center lines are mean±SEM (n=5 biologically independent samples). Mann-Whitney test for significance: ** p =0.0079, two-tailed. b-h , Lignan levels in mice dosed with b-e , PINO (10 mg/kg) or f-h , PINO-diglucoside (20 mg/kg) measured by Orbitrap mass spectrometry. Panels b,c,f,g represent END levels. Panels d,h represent ENL levels. Panel e represents PINO levels. Values are mean±SEM (n=5 biologically independent samples/colonization group with the following exceptions: b-e , 18-hr urine ( ber + , n= 4 biologically independent samples), colon (GF, n=4 biologically independent samples; ber − , n=3 biologically independent samples), and serum at 6-hr timepoint ( ber + , n= 4 biologically independent samples); g , ileum ( ber + , n= 4 biologically independent samples). Kruskal-Wallis with Dunn’s multiple comparisons test: * p <0.05, ** p <0.01, *** p <0.001. ns: not statistically significant. ber + and ber − : germ-free mice colonized with E. lenta DSM2243 T ( ber + group) or E. lenta 1–3-56 ( ber − group) and B. producta DSM3507, G. pamelaeae 3C, and L. longoviformis DSM17459 T ; mice dosed with PINO-diglucoside were also colonized with C. saccharogumia DSM17460 T . GF: germ-free mice.
Article Snippet:
Techniques: Quantitation Assay, MANN-WHITNEY, Two Tailed Test, Mass Spectrometry