6 fam Thermo Fisher Search Results


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  • 80
    Thermo Fisher phosphoramidite dye 6 fam
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    Effect of CDDO-Me on MDSC in vivo A. Tumors were established subcutaneously in C57BL/6 mice. Two weeks after tumor inoculation, mice were treated with either control or CDDO-Me (60-150 mg/kg chow) food for the indicated periods (grey line). Tumor size was monitored every 2-3 days. Mean and SD of tumor size is shown. Each group includes 5 mice. B. RNA was extracted from MDSC and the expression of <t>NQO1</t> was measured by qRT-PCR. The level of ROS in MDSC in CDDO-Me-treated tumor-bearing mice was measured using DCFDA staining and flow cytometry. C. Splenocytes from MC38, EL-4, or LLC tumor-bearing mice treated with CDDO-Me for 7 days were stained with anti-CD11b and anti-Gr-1 antibodies and the percentage of Gr-1 + CD11b + cells was analyzed using flow cytometry. Each group included 3 mice. * - statistically significant differences from control (p
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    Effect of CDDO-Me on MDSC in vivo A. Tumors were established subcutaneously in C57BL/6 mice. Two weeks after tumor inoculation, mice were treated with either control or CDDO-Me (60-150 mg/kg chow) food for the indicated periods (grey line). Tumor size was monitored every 2-3 days. Mean and SD of tumor size is shown. Each group includes 5 mice. B. RNA was extracted from MDSC and the expression of <t>NQO1</t> was measured by qRT-PCR. The level of ROS in MDSC in CDDO-Me-treated tumor-bearing mice was measured using DCFDA staining and flow cytometry. C. Splenocytes from MC38, EL-4, or LLC tumor-bearing mice treated with CDDO-Me for 7 days were stained with anti-CD11b and anti-Gr-1 antibodies and the percentage of Gr-1 + CD11b + cells was analyzed using flow cytometry. Each group included 3 mice. * - statistically significant differences from control (p
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    Image Search Results


    A linkage disequilibrium plot for the eight most common SNPs within the EMSY gene Pairwise LD (R 2 ) is shown for each combination of SNPs.

    Journal: Oncotarget

    Article Title: Clinical importance of the EMSY gene expression and polymorphisms in ovarian cancer

    doi: 10.18632/oncotarget.24878

    Figure Lengend Snippet: A linkage disequilibrium plot for the eight most common SNPs within the EMSY gene Pairwise LD (R 2 ) is shown for each combination of SNPs.

    Article Snippet: Gene expression was evaluated with the following TaqMan assays: id: Hs00220187_m1 ( EMSY -specific, 6-FAM-labeled, Life Technologies), id: 4326321E ( HGPRT -specific, VIC-labeled, Life Technologies), id: 4326316E ( PPIA -specific, VIC-labeled, Life Technologies), and id: 4326320E ( GUSB -specific, VIC-labeled, Life Technologies).

    Techniques:

    Evaluation of a predictive value of the EMSY gene expression in the PC- and TP-treated subgroups of ovarian cancer patients We analyzed the relationship between EMSY expression and sensitivity to treatment (PS). According to the multivariate logistic regression model, this regularity was statistically significant only in the TP-treated subgroup. Non-significant – a result with a p-value ≥0.1.

    Journal: Oncotarget

    Article Title: Clinical importance of the EMSY gene expression and polymorphisms in ovarian cancer

    doi: 10.18632/oncotarget.24878

    Figure Lengend Snippet: Evaluation of a predictive value of the EMSY gene expression in the PC- and TP-treated subgroups of ovarian cancer patients We analyzed the relationship between EMSY expression and sensitivity to treatment (PS). According to the multivariate logistic regression model, this regularity was statistically significant only in the TP-treated subgroup. Non-significant – a result with a p-value ≥0.1.

    Article Snippet: Gene expression was evaluated with the following TaqMan assays: id: Hs00220187_m1 ( EMSY -specific, 6-FAM-labeled, Life Technologies), id: 4326321E ( HGPRT -specific, VIC-labeled, Life Technologies), id: 4326316E ( PPIA -specific, VIC-labeled, Life Technologies), and id: 4326320E ( GUSB -specific, VIC-labeled, Life Technologies).

    Techniques: Expressing

    Effect of CDDO-Me on MDSC in vivo A. Tumors were established subcutaneously in C57BL/6 mice. Two weeks after tumor inoculation, mice were treated with either control or CDDO-Me (60-150 mg/kg chow) food for the indicated periods (grey line). Tumor size was monitored every 2-3 days. Mean and SD of tumor size is shown. Each group includes 5 mice. B. RNA was extracted from MDSC and the expression of NQO1 was measured by qRT-PCR. The level of ROS in MDSC in CDDO-Me-treated tumor-bearing mice was measured using DCFDA staining and flow cytometry. C. Splenocytes from MC38, EL-4, or LLC tumor-bearing mice treated with CDDO-Me for 7 days were stained with anti-CD11b and anti-Gr-1 antibodies and the percentage of Gr-1 + CD11b + cells was analyzed using flow cytometry. Each group included 3 mice. * - statistically significant differences from control (p

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Anti-inflammatory triterpenoid blocks immune suppressive function of myeloid-derived suppressor cells and improves immune response in cancer

    doi: 10.1158/1078-0432.CCR-09-3272

    Figure Lengend Snippet: Effect of CDDO-Me on MDSC in vivo A. Tumors were established subcutaneously in C57BL/6 mice. Two weeks after tumor inoculation, mice were treated with either control or CDDO-Me (60-150 mg/kg chow) food for the indicated periods (grey line). Tumor size was monitored every 2-3 days. Mean and SD of tumor size is shown. Each group includes 5 mice. B. RNA was extracted from MDSC and the expression of NQO1 was measured by qRT-PCR. The level of ROS in MDSC in CDDO-Me-treated tumor-bearing mice was measured using DCFDA staining and flow cytometry. C. Splenocytes from MC38, EL-4, or LLC tumor-bearing mice treated with CDDO-Me for 7 days were stained with anti-CD11b and anti-Gr-1 antibodies and the percentage of Gr-1 + CD11b + cells was analyzed using flow cytometry. Each group included 3 mice. * - statistically significant differences from control (p

    Article Snippet: PCR was performed with 2.5 μl cDNA, TaqMan Universal PCR Master Mix (Applied Biosystems, Foster City, CA), and target gene assay mix containing sequence-specific primers for NQO1 and 6-carboxyfluorescein (6-FAM) dye-labeled TaqMan minor groove binder (MGB) probe (Assay ID: Mm00500821_m1, Applied Biosystems, Foster City, CA).

    Techniques: In Vivo, Mouse Assay, Expressing, Quantitative RT-PCR, Staining, Flow Cytometry, Cytometry