Journal: Science signaling

Article Title: Allosteric signaling through an mGlu2 and 5-HT2A heteromeric receptor complex and its potential contribution to schizophrenia.

doi: 10.1126/scisignal.aab0467

Figure Lengend Snippet: Fig. 5. Model for the docking of Gi/o onto the 5-HT2A–mGlu2 heteromeric receptor complex. (A to C) Ribbon representation of the vertical (A), extracellular (B), and cytoplasmic (C) views of the mechanism by which coupling of Gi/o proteins to the mGlu2 protomer located distal to the 5-HT2A component is necessary to enable allosteric crosstalk with 5-HT2A and consequently activate Gq/11- dependent signaling. The homodimeric interfaces of mGlu2 and 5-HT2A involve residues from TM1. The interface of the 5-HT2A–mGlu2 heteromeric complex involves residues from TM4. aAH, a-helical domain of the Ga subunit; aRas, Ras-like domain of the Ga subunit.

Article Snippet: For confirmation of specificity of the primary antibody (catalog no. RA24288; Neuromics) against 5-HT2A receptors in experiments with knockout mice, see fig. S9.

Techniques:

Journal: Biomolecules

Article Title: The Novel Pimavanserin Derivative ST-2300 with Histamine H 3 Receptor Affinity Shows Reduced 5-HT 2A Binding, but Maintains Antidepressant- and Anxiolytic-like Properties in Mice

doi: 10.3390/biom12050683

Figure Lengend Snippet: Binding affinity or percentage inhibition of ST-2300 or PIM at different GPCRs.

Article Snippet: HEK293 cells with a stable expression of human 5-HT 1A , 5-HT 6 , 5-HT 7b , and D 2L (prepared with the use of Lipofectamine 2000), or CHO-K1 cells with plasmids containing the sequence coding for the human serotonin 5-HT 2A receptor (PerkinElmer), were maintained at 37 °C in a humidified atmosphere with 5% CO 2 and grown in Dulbecco’s Modified Eagle Medium containing 10% dialyzed fetal bovine serum and 500 μg/mL G418 sulfate.

Techniques: Binding Assay, Inhibition

Journal: Journal of Microbiology and Biotechnology

Article Title: The Effect of Lactobacillus gasseri BNR17 on Postmenopausal Symptoms in Ovariectomized Rats

doi: 10.4014/jmb.2105.05032

Figure Lengend Snippet: Serum estradiol (E2) ( A ), Calcitonin ( B ), Osteocalcin ( C ), and 5-HT-2A ( D ) were measured after 14 weeks of initial administration ( n = 6~8 rats per group). Sophora japonica fruit extract (SJFE) treatment was used to create the positive control.

Article Snippet: The concentrations of estradiol (E2, CSB-E05110r), osteocalcin (CSB-E05129r), calcitonin (CSB-E05132r), estradiol (CSB-E05110r), and Serotonin 2A (5-HT-2A, CSB-E14956r) were assessed using an ELISA kit (CUSABIO, China).

Techniques: Positive Control