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prps2  (Novus Biologicals)
92
Novus Biologicals prps2
Prps2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prps2/product/Novus Biologicals
Average 92 stars, based on 1 article reviews
prps2 - by Bioz Stars, 2026-02
92/100 stars
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e2f3  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology e2f3
Fig. 5 miR-34a-5p inhibitor reduces hyperoxia-induced KLF4 protein expression in cultured lung epithelial cells. MLE-12 cells were transfected with miR-34a-5p inhibitor (Inh) or negative control (NC) at 20 nM. 24 h after transfection, MLE-12 cells were exposed to 21% O2/5% CO2 (Air) or 95% O2/5% CO2 (O2) for 24 h. A–D <t>E2F3,</t> E2F1, CCND1, and KLF4 protein levels were measured by Western blot. Densitometry of bands was normalized using calnexin (CNX) levels. E Transcription levels of Klf4 were measured by RT-qPCR. Data are expressed as mean ± SEM (n = 6). *p < 0.05, **p < 0.01, ***p < 0.001 versus air NC, group, †p < 0.05 versus NC in O2 group, RT-qPCR, quantitative real-time PCR
E2f3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e2f3/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
e2f3 - by Bioz Stars, 2026-02
94/100 stars
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Fig. 5 miR-34a-5p inhibitor reduces hyperoxia-induced KLF4 protein expression in cultured lung epithelial cells. MLE-12 cells were transfected with miR-34a-5p inhibitor (Inh) or negative control (NC) at 20 nM. 24 h after transfection, MLE-12 cells were exposed to 21% O2/5% CO2 (Air) or 95% O2/5% CO2 (O2) for 24 h. A–D E2F3, E2F1, CCND1, and KLF4 protein levels were measured by Western blot. Densitometry of bands was normalized using calnexin (CNX) levels. E Transcription levels of Klf4 were measured by RT-qPCR. Data are expressed as mean ± SEM (n = 6). *p < 0.05, **p < 0.01, ***p < 0.001 versus air NC, group, †p < 0.05 versus NC in O2 group, RT-qPCR, quantitative real-time PCR

Journal: Respiratory research

Article Title: Involvement of miRNA-34a regulated Krüppel-like factor 4 expression in hyperoxia-induced senescence in lung epithelial cells.

doi: 10.1186/s12931-022-02263-8

Figure Lengend Snippet: Fig. 5 miR-34a-5p inhibitor reduces hyperoxia-induced KLF4 protein expression in cultured lung epithelial cells. MLE-12 cells were transfected with miR-34a-5p inhibitor (Inh) or negative control (NC) at 20 nM. 24 h after transfection, MLE-12 cells were exposed to 21% O2/5% CO2 (Air) or 95% O2/5% CO2 (O2) for 24 h. A–D E2F3, E2F1, CCND1, and KLF4 protein levels were measured by Western blot. Densitometry of bands was normalized using calnexin (CNX) levels. E Transcription levels of Klf4 were measured by RT-qPCR. Data are expressed as mean ± SEM (n = 6). *p < 0.05, **p < 0.01, ***p < 0.001 versus air NC, group, †p < 0.05 versus NC in O2 group, RT-qPCR, quantitative real-time PCR

Article Snippet: The membranes were blocked for 1 h at room temperature with 5% milk and then probed with primary antibodies against p53 (ab131442, Abcam), p21 (ab109199, Abcam), p16 (ab108349, Abcam), MDM4 (ab222905, Abcam or sc-74468, Santa Cruz), E2F1 (ab137415, Abcam), E2F3 (sc-56665, Santa Cruz), CCND1 (ab134175, Abcam), KLF4 (ab129473, Abcam), β-actin (ab8227, Abcam) and calnexin (ADI-SPA-860-F, Enzo, New York, NY, USA) to determine the corresponding proteins.

Techniques: Expressing, Cell Culture, Transfection, Negative Control, Western Blot, Quantitative RT-PCR, Real-time Polymerase Chain Reaction