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rcas1  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology rcas1
Expression of Limb Girdle Muscular Dystrophy‐1C Cav3 mutation (P104L) localizes Cav3 to the Golgi and causes mitochondrial fragmentation. L6 myoblasts expressing WT‐Cav3‐GFP or Cav3 P104L ‐GFP were fixed and immunostained for <t>RCAS1</t> (red, a Golgi marker) and stained with DAPI (blue, nuclear stain) while wild‐type (WT) cells not expressing GFP were immunostained using antibodies to Cav3 (green), and visualized using confocal microscopy, on three separate experiments with a minimum of 6 images per sample (A). L6 myoblasts stably expressing an empty GFP vector, WT‐Cav3‐GFP or Cav3 P104L ‐GFP were stained with Mitotracker Deep Red and visualized using live cell confocal microscopy. Enlarged images (derived from the fields within the indicated red boxes) highlight changes in mitochondrial morphology (B). Mitochondrial length was quantified using Volocity software and presented as elongated/tubular if greater than 1 μm and fragmented if less than 1 μm in length. Data are presented as mean ± SEM from a minimum of three separate experiments with analyses of 15–20 randomly chosen visual fields for each condition in each experiment (C). Asterisks indicate a relative significant change ( P < 0.05) between the indicated bars. Cav3, caveolin‐3; GFP, green fluorescent protein.
Rcas1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rcas1/product/Santa Cruz Biotechnology
Average 92 stars, based on 1 article reviews
rcas1 - by Bioz Stars, 2026-02
92/100 stars
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placental cdna libraries  (Unigene)
90
Unigene placental cdna libraries
Expression of Limb Girdle Muscular Dystrophy‐1C Cav3 mutation (P104L) localizes Cav3 to the Golgi and causes mitochondrial fragmentation. L6 myoblasts expressing WT‐Cav3‐GFP or Cav3 P104L ‐GFP were fixed and immunostained for <t>RCAS1</t> (red, a Golgi marker) and stained with DAPI (blue, nuclear stain) while wild‐type (WT) cells not expressing GFP were immunostained using antibodies to Cav3 (green), and visualized using confocal microscopy, on three separate experiments with a minimum of 6 images per sample (A). L6 myoblasts stably expressing an empty GFP vector, WT‐Cav3‐GFP or Cav3 P104L ‐GFP were stained with Mitotracker Deep Red and visualized using live cell confocal microscopy. Enlarged images (derived from the fields within the indicated red boxes) highlight changes in mitochondrial morphology (B). Mitochondrial length was quantified using Volocity software and presented as elongated/tubular if greater than 1 μm and fragmented if less than 1 μm in length. Data are presented as mean ± SEM from a minimum of three separate experiments with analyses of 15–20 randomly chosen visual fields for each condition in each experiment (C). Asterisks indicate a relative significant change ( P < 0.05) between the indicated bars. Cav3, caveolin‐3; GFP, green fluorescent protein.
Placental Cdna Libraries, supplied by Unigene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/placental cdna libraries/product/Unigene
Average 90 stars, based on 1 article reviews
placental cdna libraries - by Bioz Stars, 2026-02
90/100 stars
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recombinant human baff carrier free  (BioLegend)
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Recombinant Human BAFF carrier free Apps BA Size 10 µg
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unc13c polyclonal antibody  (Thermo Fisher)
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UNC13C Polyclonal Antibody for IHC
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piperidin 1 yl 1h pyrazol 4 yl methanone  (BOC Sciences)
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galns antibody  (ProSci Incorporated)
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GALNS Antibody raised in Rabbit validated in WB IHC P IF Flow in Human
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methyl 2 chloroquinazoline 6 carboxylate  (BOC Sciences)
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Methyl 2 chloroquinazoline 6 carboxylate CAS 1036755 96 0 is a useful research chemical
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reg iiiγ m pr  (Santa Cruz Biotechnology)
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Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of Reg IIIγ gene silencing results individual duplex components or plasmids are also available
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shrna clone set against human klk11  (Genecopoeia)
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OmicsLink™ shRNA clone collections include lentiviral and non-viral vector-based shRNA constructs against genome-wide human, mouse and rat genes. shRNA of varying lengths (19 to 29 bases) were designed using a proprietary algorithm to make shRNA
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c1orf87 antibody  (biorbyt)
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Rabbit polyclonal antibody to C1orf87 Isotype Note IgG Host Note Rabbit Conjugation Note Unconjugated Reactivity Note Human Mouse Rat Application Note WB IHC P P ELISA
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rgd1559600 rat shrna plasmid  (OriGene)
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RGD1559600 Rat 4 unique 29mer shRNA constructs in retroviral untagged vector
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purified mouse anti pten  (BD Biosciences)
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Image Search Results


Expression of Limb Girdle Muscular Dystrophy‐1C Cav3 mutation (P104L) localizes Cav3 to the Golgi and causes mitochondrial fragmentation. L6 myoblasts expressing WT‐Cav3‐GFP or Cav3 P104L ‐GFP were fixed and immunostained for RCAS1 (red, a Golgi marker) and stained with DAPI (blue, nuclear stain) while wild‐type (WT) cells not expressing GFP were immunostained using antibodies to Cav3 (green), and visualized using confocal microscopy, on three separate experiments with a minimum of 6 images per sample (A). L6 myoblasts stably expressing an empty GFP vector, WT‐Cav3‐GFP or Cav3 P104L ‐GFP were stained with Mitotracker Deep Red and visualized using live cell confocal microscopy. Enlarged images (derived from the fields within the indicated red boxes) highlight changes in mitochondrial morphology (B). Mitochondrial length was quantified using Volocity software and presented as elongated/tubular if greater than 1 μm and fragmented if less than 1 μm in length. Data are presented as mean ± SEM from a minimum of three separate experiments with analyses of 15–20 randomly chosen visual fields for each condition in each experiment (C). Asterisks indicate a relative significant change ( P < 0.05) between the indicated bars. Cav3, caveolin‐3; GFP, green fluorescent protein.

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: Caveolin‐3 deficiency associated with the dystrophy P104L mutation impairs skeletal muscle mitochondrial form and function

doi: 10.1002/jcsm.12541

Figure Lengend Snippet: Expression of Limb Girdle Muscular Dystrophy‐1C Cav3 mutation (P104L) localizes Cav3 to the Golgi and causes mitochondrial fragmentation. L6 myoblasts expressing WT‐Cav3‐GFP or Cav3 P104L ‐GFP were fixed and immunostained for RCAS1 (red, a Golgi marker) and stained with DAPI (blue, nuclear stain) while wild‐type (WT) cells not expressing GFP were immunostained using antibodies to Cav3 (green), and visualized using confocal microscopy, on three separate experiments with a minimum of 6 images per sample (A). L6 myoblasts stably expressing an empty GFP vector, WT‐Cav3‐GFP or Cav3 P104L ‐GFP were stained with Mitotracker Deep Red and visualized using live cell confocal microscopy. Enlarged images (derived from the fields within the indicated red boxes) highlight changes in mitochondrial morphology (B). Mitochondrial length was quantified using Volocity software and presented as elongated/tubular if greater than 1 μm and fragmented if less than 1 μm in length. Data are presented as mean ± SEM from a minimum of three separate experiments with analyses of 15–20 randomly chosen visual fields for each condition in each experiment (C). Asterisks indicate a relative significant change ( P < 0.05) between the indicated bars. Cav3, caveolin‐3; GFP, green fluorescent protein.

Article Snippet: Cell lysates and membrane preparations were subject to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) and separated proteins transferred onto PVDF membrane (Millipore) prior to incubation with the following primary antibodies at a dilution of 1:1000 except where indicated otherwise: actin (#A5060, 1:5000) was obtained from Sigma; ANT‐1 (#ab180715) and PGC1α (#ab54481) were from Abcam; MFN1 (#MMS‐5021) was purchased from Biolegend; Mitofusin 2 (MFN2) (#SC100560), succinate dehyrdogenase (SDHA) (#SC98253), and GAPDH (#SC32233, 1:5000) were purchased Santa Cruz; TOM20 (# 42406S), voltage‐dependent anion channel (VDAC) (#4661S), HA (#2367S), BiP (#C50B12), COX4 (#4580S), Catalase (#14097), Cytochrome C (#11940), RCAS1 (#12290), and manganase superoxide dismutase (MnSOD) (#D9V9C) were all purchased from Cell Signalling Technology; Cav1 (#610058), Cav3 (#610421), DLP1 /Drp1 (#611112), and OPA1 (#612607) were from BD Biosciences.

Techniques: Expressing, Mutagenesis, Marker, Staining, Confocal Microscopy, Stable Transfection, Plasmid Preparation, Derivative Assay, Software