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Image Search Results
Journal:
Article Title: Specific Detection and Prevalence of Helicobacter heilmannii -Like Organisms in the Human Gastric Mucosa by Fluorescent In Situ Hybridization and Partial 16S Ribosomal DNA Sequencing
doi: 10.1128/JCM.39.4.1510-1516.2001
Figure Lengend Snippet: Sequences of specific primers and probes for FISH and PCR
Article Snippet: The following bacterial strains were used to evaluate specificity of PCR primers and hybridization probes developed for this study: H. pylori (NCTC 11637), Helicobacter acinonychis (CCUG11284), Helicobacter nemestrinae (ATCC 49396) Helicobacter salomonis (CCUG37845), Helicobacter felis CS1 (ATCC 49179),
Techniques: Sequencing, Bacteria
Journal:
Article Title: Specific Detection and Prevalence of Helicobacter heilmannii -Like Organisms in the Human Gastric Mucosa by Fluorescent In Situ Hybridization and Partial 16S Ribosomal DNA Sequencing
doi: 10.1128/JCM.39.4.1510-1516.2001
Figure Lengend Snippet: Formalin-fixed gastric sections of patients with “H. heilmannii” types 1 and 2 and HHLOs detected by FISH using the oligonucleotide probes summarized in Table Table1.1. (A) “H. heilmannii” type 1; (B) “H. heilmannii ” type 2; (C) HHLO-4; (D) HHLO-5; (E and F) H. pylori. In panels A to D the Eub-338 probe labeled in green (Fluos) was used together with the corresponding HHLO type-specific probe, labeled in red (Cy-3). The mixed yellow and orange color indicates binding of both probes simultaneously. (D) A mixed infection with “H. heilmannii ” type 1 and HHLO-5 is demonstrated; one Helicobacter isolate (yellow-orange) binds the Eub-388 and HHLO-1 probes, and the other bacteria bind the Eub338 probe only and represent HHLO-5 organisms, as specified with the corresponding probe (not shown). DNA is labeled with DAPI (in blue). (E and F) Gastric sections with H. pylori were used to demonstrate the specificity of the probes. The Hhe-1 probe is labeled in red (Cy-3) (E), and the Hpy-1 probe is labeled in green (Fluos) (F).
Article Snippet: The following bacterial strains were used to evaluate specificity of PCR primers and hybridization probes developed for this study: H. pylori (NCTC 11637), Helicobacter acinonychis (CCUG11284), Helicobacter nemestrinae (ATCC 49396) Helicobacter salomonis (CCUG37845), Helicobacter felis CS1 (ATCC 49179),
Techniques: Labeling, Binding Assay, Infection, Bacteria