5.3a Search Results


t2877 t3 chem impex int  (Chem Impex International)
95
Chem Impex International t2877 t3 chem impex int
T2877 T3 Chem Impex Int, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%2E3a/pm27720451-494-98-100?v=Chem+Impex+International
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t2877 t3 chem impex int - by Bioz Stars, 2026-07
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antibodies to deptor  (Proteintech)
93
Proteintech antibodies to deptor
Figure 4 Baf60c is required for maintaining glycolytic metabolism in adult skeletal muscle. (a) <t>Deptor</t> mRNA expression in C2C12 myotubes transduced with the indicated adenoviral vectors. (b) Reporter gene assay with constructs containing either WT Deptor promoter or a Deptor promoter mutated at the Six4 binding site. (c) Relative enrichment of Baf60c and Six4 at the putative Six4 binding site on the proximal Deptor promoter. (d) Physical interaction of Six4 and Baf60c in transiently transfected HEK293T cells. IP, immunoprecipitation; Myc-Baf60c, Myc-tagged Baf60c; FH-Six4, Flag- and HA-tagged Six4. (e) ChIP assay in transduced myotubes <t>using</t> <t>antibodies</t> to H3K4me3 and H3K9me2. The locations of the qPCR primers are relative to the transcriptional start site of Deptor. (f) Baf60c and Deptor mRNA levels in tibialis anterior muscle transduced with indicated adenoviral vectors (n = 6 mice per group) (left) and immunoblots of total protein lysates from transduced tibialis anterior muscle (right). Shown are representative blots of two mice. (g) LDH enzymatic activity in transduced tibialis anterior muscle (n = 8 mice per group). (h) Representative histochemical staining of COX (left), SDH (middle) and α-GPDH (right) activities on frozen sections of transduced tibialis anterior muscle (n = 8 mice per group). Scale bar, 100 µm. (i) Model depicting the Baf60c-Deptor-Akt regulatory cascade in metabolic and contractile specification of fast-twitch glycolytic muscle. Data are shown as the mean ± s.d. and are representative of three independent experiments (a–c,e) or the mean ± s.e.m. (f,g). *P < 0.01 by two-tailed Student’s t test.
Antibodies To Deptor, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%2E3a/pm23563706-334-0-13?v=Proteintech
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antibodies to deptor - by Bioz Stars, 2026-07
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protein sequencer  (Shimadzu Corporation)
94
Shimadzu Corporation protein sequencer
Figure 4 Baf60c is required for maintaining glycolytic metabolism in adult skeletal muscle. (a) <t>Deptor</t> mRNA expression in C2C12 myotubes transduced with the indicated adenoviral vectors. (b) Reporter gene assay with constructs containing either WT Deptor promoter or a Deptor promoter mutated at the Six4 binding site. (c) Relative enrichment of Baf60c and Six4 at the putative Six4 binding site on the proximal Deptor promoter. (d) Physical interaction of Six4 and Baf60c in transiently transfected HEK293T cells. IP, immunoprecipitation; Myc-Baf60c, Myc-tagged Baf60c; FH-Six4, Flag- and HA-tagged Six4. (e) ChIP assay in transduced myotubes <t>using</t> <t>antibodies</t> to H3K4me3 and H3K9me2. The locations of the qPCR primers are relative to the transcriptional start site of Deptor. (f) Baf60c and Deptor mRNA levels in tibialis anterior muscle transduced with indicated adenoviral vectors (n = 6 mice per group) (left) and immunoblots of total protein lysates from transduced tibialis anterior muscle (right). Shown are representative blots of two mice. (g) LDH enzymatic activity in transduced tibialis anterior muscle (n = 8 mice per group). (h) Representative histochemical staining of COX (left), SDH (middle) and α-GPDH (right) activities on frozen sections of transduced tibialis anterior muscle (n = 8 mice per group). Scale bar, 100 µm. (i) Model depicting the Baf60c-Deptor-Akt regulatory cascade in metabolic and contractile specification of fast-twitch glycolytic muscle. Data are shown as the mean ± s.d. and are representative of three independent experiments (a–c,e) or the mean ± s.e.m. (f,g). *P < 0.01 by two-tailed Student’s t test.
Protein Sequencer, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%2E3a/pm08903405-70-22-26?v=Shimadzu+Corporation
Average 94 stars, based on 1 article reviews
protein sequencer - by Bioz Stars, 2026-07
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computational fluid dynamics module comsol multiphysics 5.3a two-phase flow level set interface  (COMSOL Inc)
90
COMSOL Inc computational fluid dynamics module comsol multiphysics 5.3a two-phase flow level set interface
Figure 4 Baf60c is required for maintaining glycolytic metabolism in adult skeletal muscle. (a) <t>Deptor</t> mRNA expression in C2C12 myotubes transduced with the indicated adenoviral vectors. (b) Reporter gene assay with constructs containing either WT Deptor promoter or a Deptor promoter mutated at the Six4 binding site. (c) Relative enrichment of Baf60c and Six4 at the putative Six4 binding site on the proximal Deptor promoter. (d) Physical interaction of Six4 and Baf60c in transiently transfected HEK293T cells. IP, immunoprecipitation; Myc-Baf60c, Myc-tagged Baf60c; FH-Six4, Flag- and HA-tagged Six4. (e) ChIP assay in transduced myotubes <t>using</t> <t>antibodies</t> to H3K4me3 and H3K9me2. The locations of the qPCR primers are relative to the transcriptional start site of Deptor. (f) Baf60c and Deptor mRNA levels in tibialis anterior muscle transduced with indicated adenoviral vectors (n = 6 mice per group) (left) and immunoblots of total protein lysates from transduced tibialis anterior muscle (right). Shown are representative blots of two mice. (g) LDH enzymatic activity in transduced tibialis anterior muscle (n = 8 mice per group). (h) Representative histochemical staining of COX (left), SDH (middle) and α-GPDH (right) activities on frozen sections of transduced tibialis anterior muscle (n = 8 mice per group). Scale bar, 100 µm. (i) Model depicting the Baf60c-Deptor-Akt regulatory cascade in metabolic and contractile specification of fast-twitch glycolytic muscle. Data are shown as the mean ± s.d. and are representative of three independent experiments (a–c,e) or the mean ± s.e.m. (f,g). *P < 0.01 by two-tailed Student’s t test.
Computational Fluid Dynamics Module Comsol Multiphysics 5.3a Two Phase Flow Level Set Interface, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%2E3a/pmc08139589-209-0-4?v=COMSOL+Inc
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computational fluid dynamics module comsol multiphysics 5.3a two-phase flow level set interface - by Bioz Stars, 2026-07
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transient flow simulation comsol multiphysics 5.3a  (COMSOL Inc)
90
COMSOL Inc transient flow simulation comsol multiphysics 5.3a
Figure 4 Baf60c is required for maintaining glycolytic metabolism in adult skeletal muscle. (a) <t>Deptor</t> mRNA expression in C2C12 myotubes transduced with the indicated adenoviral vectors. (b) Reporter gene assay with constructs containing either WT Deptor promoter or a Deptor promoter mutated at the Six4 binding site. (c) Relative enrichment of Baf60c and Six4 at the putative Six4 binding site on the proximal Deptor promoter. (d) Physical interaction of Six4 and Baf60c in transiently transfected HEK293T cells. IP, immunoprecipitation; Myc-Baf60c, Myc-tagged Baf60c; FH-Six4, Flag- and HA-tagged Six4. (e) ChIP assay in transduced myotubes <t>using</t> <t>antibodies</t> to H3K4me3 and H3K9me2. The locations of the qPCR primers are relative to the transcriptional start site of Deptor. (f) Baf60c and Deptor mRNA levels in tibialis anterior muscle transduced with indicated adenoviral vectors (n = 6 mice per group) (left) and immunoblots of total protein lysates from transduced tibialis anterior muscle (right). Shown are representative blots of two mice. (g) LDH enzymatic activity in transduced tibialis anterior muscle (n = 8 mice per group). (h) Representative histochemical staining of COX (left), SDH (middle) and α-GPDH (right) activities on frozen sections of transduced tibialis anterior muscle (n = 8 mice per group). Scale bar, 100 µm. (i) Model depicting the Baf60c-Deptor-Akt regulatory cascade in metabolic and contractile specification of fast-twitch glycolytic muscle. Data are shown as the mean ± s.d. and are representative of three independent experiments (a–c,e) or the mean ± s.e.m. (f,g). *P < 0.01 by two-tailed Student’s t test.
Transient Flow Simulation Comsol Multiphysics 5.3a, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%2E3a/pmc09017991-192-0-3?v=COMSOL+Inc
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transient flow simulation comsol multiphysics 5.3a - by Bioz Stars, 2026-07
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version 5.3a  (COMSOL Inc)
90
COMSOL Inc version 5.3a
Figure 4 Baf60c is required for maintaining glycolytic metabolism in adult skeletal muscle. (a) <t>Deptor</t> mRNA expression in C2C12 myotubes transduced with the indicated adenoviral vectors. (b) Reporter gene assay with constructs containing either WT Deptor promoter or a Deptor promoter mutated at the Six4 binding site. (c) Relative enrichment of Baf60c and Six4 at the putative Six4 binding site on the proximal Deptor promoter. (d) Physical interaction of Six4 and Baf60c in transiently transfected HEK293T cells. IP, immunoprecipitation; Myc-Baf60c, Myc-tagged Baf60c; FH-Six4, Flag- and HA-tagged Six4. (e) ChIP assay in transduced myotubes <t>using</t> <t>antibodies</t> to H3K4me3 and H3K9me2. The locations of the qPCR primers are relative to the transcriptional start site of Deptor. (f) Baf60c and Deptor mRNA levels in tibialis anterior muscle transduced with indicated adenoviral vectors (n = 6 mice per group) (left) and immunoblots of total protein lysates from transduced tibialis anterior muscle (right). Shown are representative blots of two mice. (g) LDH enzymatic activity in transduced tibialis anterior muscle (n = 8 mice per group). (h) Representative histochemical staining of COX (left), SDH (middle) and α-GPDH (right) activities on frozen sections of transduced tibialis anterior muscle (n = 8 mice per group). Scale bar, 100 µm. (i) Model depicting the Baf60c-Deptor-Akt regulatory cascade in metabolic and contractile specification of fast-twitch glycolytic muscle. Data are shown as the mean ± s.d. and are representative of three independent experiments (a–c,e) or the mean ± s.e.m. (f,g). *P < 0.01 by two-tailed Student’s t test.
Version 5.3a, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%2E3a/pm36517538-177-19-18?v=COMSOL+Inc
Average 90 stars, based on 1 article reviews
version 5.3a - by Bioz Stars, 2026-07
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multiphysics 53a fem  (COMSOL Inc)
90
COMSOL Inc multiphysics 53a fem
Figure 4 Baf60c is required for maintaining glycolytic metabolism in adult skeletal muscle. (a) <t>Deptor</t> mRNA expression in C2C12 myotubes transduced with the indicated adenoviral vectors. (b) Reporter gene assay with constructs containing either WT Deptor promoter or a Deptor promoter mutated at the Six4 binding site. (c) Relative enrichment of Baf60c and Six4 at the putative Six4 binding site on the proximal Deptor promoter. (d) Physical interaction of Six4 and Baf60c in transiently transfected HEK293T cells. IP, immunoprecipitation; Myc-Baf60c, Myc-tagged Baf60c; FH-Six4, Flag- and HA-tagged Six4. (e) ChIP assay in transduced myotubes <t>using</t> <t>antibodies</t> to H3K4me3 and H3K9me2. The locations of the qPCR primers are relative to the transcriptional start site of Deptor. (f) Baf60c and Deptor mRNA levels in tibialis anterior muscle transduced with indicated adenoviral vectors (n = 6 mice per group) (left) and immunoblots of total protein lysates from transduced tibialis anterior muscle (right). Shown are representative blots of two mice. (g) LDH enzymatic activity in transduced tibialis anterior muscle (n = 8 mice per group). (h) Representative histochemical staining of COX (left), SDH (middle) and α-GPDH (right) activities on frozen sections of transduced tibialis anterior muscle (n = 8 mice per group). Scale bar, 100 µm. (i) Model depicting the Baf60c-Deptor-Akt regulatory cascade in metabolic and contractile specification of fast-twitch glycolytic muscle. Data are shown as the mean ± s.d. and are representative of three independent experiments (a–c,e) or the mean ± s.e.m. (f,g). *P < 0.01 by two-tailed Student’s t test.
Multiphysics 53a Fem, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%2E3a/graham_thomas_john__2018__underwater_acoustic_waves_on_structured_and_unstructured_plates-329-3-3?v=COMSOL+Inc
Average 90 stars, based on 1 article reviews
multiphysics 53a fem - by Bioz Stars, 2026-07
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refractometer's prism master-53a  (ATAGO CO)
90
ATAGO CO refractometer's prism master-53a
Figure 4 Baf60c is required for maintaining glycolytic metabolism in adult skeletal muscle. (a) <t>Deptor</t> mRNA expression in C2C12 myotubes transduced with the indicated adenoviral vectors. (b) Reporter gene assay with constructs containing either WT Deptor promoter or a Deptor promoter mutated at the Six4 binding site. (c) Relative enrichment of Baf60c and Six4 at the putative Six4 binding site on the proximal Deptor promoter. (d) Physical interaction of Six4 and Baf60c in transiently transfected HEK293T cells. IP, immunoprecipitation; Myc-Baf60c, Myc-tagged Baf60c; FH-Six4, Flag- and HA-tagged Six4. (e) ChIP assay in transduced myotubes <t>using</t> <t>antibodies</t> to H3K4me3 and H3K9me2. The locations of the qPCR primers are relative to the transcriptional start site of Deptor. (f) Baf60c and Deptor mRNA levels in tibialis anterior muscle transduced with indicated adenoviral vectors (n = 6 mice per group) (left) and immunoblots of total protein lysates from transduced tibialis anterior muscle (right). Shown are representative blots of two mice. (g) LDH enzymatic activity in transduced tibialis anterior muscle (n = 8 mice per group). (h) Representative histochemical staining of COX (left), SDH (middle) and α-GPDH (right) activities on frozen sections of transduced tibialis anterior muscle (n = 8 mice per group). Scale bar, 100 µm. (i) Model depicting the Baf60c-Deptor-Akt regulatory cascade in metabolic and contractile specification of fast-twitch glycolytic muscle. Data are shown as the mean ± s.d. and are representative of three independent experiments (a–c,e) or the mean ± s.e.m. (f,g). *P < 0.01 by two-tailed Student’s t test.
Refractometer's Prism Master 53a, supplied by ATAGO CO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%2E3a/10__1080_slash_15538362__2021__1930626-116-16-19?v=ATAGO+CO
Average 90 stars, based on 1 article reviews
refractometer's prism master-53a - by Bioz Stars, 2026-07
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5.3a numerical analysis  (COMSOL Inc)
90
COMSOL Inc 5.3a numerical analysis
Figure 4 Baf60c is required for maintaining glycolytic metabolism in adult skeletal muscle. (a) <t>Deptor</t> mRNA expression in C2C12 myotubes transduced with the indicated adenoviral vectors. (b) Reporter gene assay with constructs containing either WT Deptor promoter or a Deptor promoter mutated at the Six4 binding site. (c) Relative enrichment of Baf60c and Six4 at the putative Six4 binding site on the proximal Deptor promoter. (d) Physical interaction of Six4 and Baf60c in transiently transfected HEK293T cells. IP, immunoprecipitation; Myc-Baf60c, Myc-tagged Baf60c; FH-Six4, Flag- and HA-tagged Six4. (e) ChIP assay in transduced myotubes <t>using</t> <t>antibodies</t> to H3K4me3 and H3K9me2. The locations of the qPCR primers are relative to the transcriptional start site of Deptor. (f) Baf60c and Deptor mRNA levels in tibialis anterior muscle transduced with indicated adenoviral vectors (n = 6 mice per group) (left) and immunoblots of total protein lysates from transduced tibialis anterior muscle (right). Shown are representative blots of two mice. (g) LDH enzymatic activity in transduced tibialis anterior muscle (n = 8 mice per group). (h) Representative histochemical staining of COX (left), SDH (middle) and α-GPDH (right) activities on frozen sections of transduced tibialis anterior muscle (n = 8 mice per group). Scale bar, 100 µm. (i) Model depicting the Baf60c-Deptor-Akt regulatory cascade in metabolic and contractile specification of fast-twitch glycolytic muscle. Data are shown as the mean ± s.d. and are representative of three independent experiments (a–c,e) or the mean ± s.e.m. (f,g). *P < 0.01 by two-tailed Student’s t test.
5.3a Numerical Analysis, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5.3a numerical analysis - by Bioz Stars, 2026-07
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5.3a  (COMSOL Inc)
90
COMSOL Inc 5.3a
Figure 4 Baf60c is required for maintaining glycolytic metabolism in adult skeletal muscle. (a) <t>Deptor</t> mRNA expression in C2C12 myotubes transduced with the indicated adenoviral vectors. (b) Reporter gene assay with constructs containing either WT Deptor promoter or a Deptor promoter mutated at the Six4 binding site. (c) Relative enrichment of Baf60c and Six4 at the putative Six4 binding site on the proximal Deptor promoter. (d) Physical interaction of Six4 and Baf60c in transiently transfected HEK293T cells. IP, immunoprecipitation; Myc-Baf60c, Myc-tagged Baf60c; FH-Six4, Flag- and HA-tagged Six4. (e) ChIP assay in transduced myotubes <t>using</t> <t>antibodies</t> to H3K4me3 and H3K9me2. The locations of the qPCR primers are relative to the transcriptional start site of Deptor. (f) Baf60c and Deptor mRNA levels in tibialis anterior muscle transduced with indicated adenoviral vectors (n = 6 mice per group) (left) and immunoblots of total protein lysates from transduced tibialis anterior muscle (right). Shown are representative blots of two mice. (g) LDH enzymatic activity in transduced tibialis anterior muscle (n = 8 mice per group). (h) Representative histochemical staining of COX (left), SDH (middle) and α-GPDH (right) activities on frozen sections of transduced tibialis anterior muscle (n = 8 mice per group). Scale bar, 100 µm. (i) Model depicting the Baf60c-Deptor-Akt regulatory cascade in metabolic and contractile specification of fast-twitch glycolytic muscle. Data are shown as the mean ± s.d. and are representative of three independent experiments (a–c,e) or the mean ± s.e.m. (f,g). *P < 0.01 by two-tailed Student’s t test.
5.3a, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%2E3a/10__1016_slash_j__nucengdes__2024__113369-101-22-22?v=COMSOL+Inc
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5.3a - by Bioz Stars, 2026-07
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fea software program bundle comsol 5.3a licensed version with rf module  (COMSOL Inc)
90
COMSOL Inc fea software program bundle comsol 5.3a licensed version with rf module
Figure 4 Baf60c is required for maintaining glycolytic metabolism in adult skeletal muscle. (a) <t>Deptor</t> mRNA expression in C2C12 myotubes transduced with the indicated adenoviral vectors. (b) Reporter gene assay with constructs containing either WT Deptor promoter or a Deptor promoter mutated at the Six4 binding site. (c) Relative enrichment of Baf60c and Six4 at the putative Six4 binding site on the proximal Deptor promoter. (d) Physical interaction of Six4 and Baf60c in transiently transfected HEK293T cells. IP, immunoprecipitation; Myc-Baf60c, Myc-tagged Baf60c; FH-Six4, Flag- and HA-tagged Six4. (e) ChIP assay in transduced myotubes <t>using</t> <t>antibodies</t> to H3K4me3 and H3K9me2. The locations of the qPCR primers are relative to the transcriptional start site of Deptor. (f) Baf60c and Deptor mRNA levels in tibialis anterior muscle transduced with indicated adenoviral vectors (n = 6 mice per group) (left) and immunoblots of total protein lysates from transduced tibialis anterior muscle (right). Shown are representative blots of two mice. (g) LDH enzymatic activity in transduced tibialis anterior muscle (n = 8 mice per group). (h) Representative histochemical staining of COX (left), SDH (middle) and α-GPDH (right) activities on frozen sections of transduced tibialis anterior muscle (n = 8 mice per group). Scale bar, 100 µm. (i) Model depicting the Baf60c-Deptor-Akt regulatory cascade in metabolic and contractile specification of fast-twitch glycolytic muscle. Data are shown as the mean ± s.d. and are representative of three independent experiments (a–c,e) or the mean ± s.e.m. (f,g). *P < 0.01 by two-tailed Student’s t test.
Fea Software Program Bundle Comsol 5.3a Licensed Version With Rf Module, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fea software program bundle comsol 5.3a licensed version with rf module - by Bioz Stars, 2026-07
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computer software platform comsol multiphysics 5.3a  (COMSOL Inc)
90
COMSOL Inc computer software platform comsol multiphysics 5.3a
Figure 4 Baf60c is required for maintaining glycolytic metabolism in adult skeletal muscle. (a) <t>Deptor</t> mRNA expression in C2C12 myotubes transduced with the indicated adenoviral vectors. (b) Reporter gene assay with constructs containing either WT Deptor promoter or a Deptor promoter mutated at the Six4 binding site. (c) Relative enrichment of Baf60c and Six4 at the putative Six4 binding site on the proximal Deptor promoter. (d) Physical interaction of Six4 and Baf60c in transiently transfected HEK293T cells. IP, immunoprecipitation; Myc-Baf60c, Myc-tagged Baf60c; FH-Six4, Flag- and HA-tagged Six4. (e) ChIP assay in transduced myotubes <t>using</t> <t>antibodies</t> to H3K4me3 and H3K9me2. The locations of the qPCR primers are relative to the transcriptional start site of Deptor. (f) Baf60c and Deptor mRNA levels in tibialis anterior muscle transduced with indicated adenoviral vectors (n = 6 mice per group) (left) and immunoblots of total protein lysates from transduced tibialis anterior muscle (right). Shown are representative blots of two mice. (g) LDH enzymatic activity in transduced tibialis anterior muscle (n = 8 mice per group). (h) Representative histochemical staining of COX (left), SDH (middle) and α-GPDH (right) activities on frozen sections of transduced tibialis anterior muscle (n = 8 mice per group). Scale bar, 100 µm. (i) Model depicting the Baf60c-Deptor-Akt regulatory cascade in metabolic and contractile specification of fast-twitch glycolytic muscle. Data are shown as the mean ± s.d. and are representative of three independent experiments (a–c,e) or the mean ± s.e.m. (f,g). *P < 0.01 by two-tailed Student’s t test.
Computer Software Platform Comsol Multiphysics 5.3a, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 4 Baf60c is required for maintaining glycolytic metabolism in adult skeletal muscle. (a) Deptor mRNA expression in C2C12 myotubes transduced with the indicated adenoviral vectors. (b) Reporter gene assay with constructs containing either WT Deptor promoter or a Deptor promoter mutated at the Six4 binding site. (c) Relative enrichment of Baf60c and Six4 at the putative Six4 binding site on the proximal Deptor promoter. (d) Physical interaction of Six4 and Baf60c in transiently transfected HEK293T cells. IP, immunoprecipitation; Myc-Baf60c, Myc-tagged Baf60c; FH-Six4, Flag- and HA-tagged Six4. (e) ChIP assay in transduced myotubes using antibodies to H3K4me3 and H3K9me2. The locations of the qPCR primers are relative to the transcriptional start site of Deptor. (f) Baf60c and Deptor mRNA levels in tibialis anterior muscle transduced with indicated adenoviral vectors (n = 6 mice per group) (left) and immunoblots of total protein lysates from transduced tibialis anterior muscle (right). Shown are representative blots of two mice. (g) LDH enzymatic activity in transduced tibialis anterior muscle (n = 8 mice per group). (h) Representative histochemical staining of COX (left), SDH (middle) and α-GPDH (right) activities on frozen sections of transduced tibialis anterior muscle (n = 8 mice per group). Scale bar, 100 µm. (i) Model depicting the Baf60c-Deptor-Akt regulatory cascade in metabolic and contractile specification of fast-twitch glycolytic muscle. Data are shown as the mean ± s.d. and are representative of three independent experiments (a–c,e) or the mean ± s.e.m. (f,g). *P < 0.01 by two-tailed Student’s t test.

Journal: Nature medicine

Article Title: Baf60c drives glycolytic metabolism in the muscle and improves systemic glucose homeostasis through Deptor-mediated Akt activation.

doi: 10.1038/nm.3144

Figure Lengend Snippet: Figure 4 Baf60c is required for maintaining glycolytic metabolism in adult skeletal muscle. (a) Deptor mRNA expression in C2C12 myotubes transduced with the indicated adenoviral vectors. (b) Reporter gene assay with constructs containing either WT Deptor promoter or a Deptor promoter mutated at the Six4 binding site. (c) Relative enrichment of Baf60c and Six4 at the putative Six4 binding site on the proximal Deptor promoter. (d) Physical interaction of Six4 and Baf60c in transiently transfected HEK293T cells. IP, immunoprecipitation; Myc-Baf60c, Myc-tagged Baf60c; FH-Six4, Flag- and HA-tagged Six4. (e) ChIP assay in transduced myotubes using antibodies to H3K4me3 and H3K9me2. The locations of the qPCR primers are relative to the transcriptional start site of Deptor. (f) Baf60c and Deptor mRNA levels in tibialis anterior muscle transduced with indicated adenoviral vectors (n = 6 mice per group) (left) and immunoblots of total protein lysates from transduced tibialis anterior muscle (right). Shown are representative blots of two mice. (g) LDH enzymatic activity in transduced tibialis anterior muscle (n = 8 mice per group). (h) Representative histochemical staining of COX (left), SDH (middle) and α-GPDH (right) activities on frozen sections of transduced tibialis anterior muscle (n = 8 mice per group). Scale bar, 100 µm. (i) Model depicting the Baf60c-Deptor-Akt regulatory cascade in metabolic and contractile specification of fast-twitch glycolytic muscle. Data are shown as the mean ± s.d. and are representative of three independent experiments (a–c,e) or the mean ± s.e.m. (f,g). *P < 0.01 by two-tailed Student’s t test.

Article Snippet: Antibodies to Deptor (1:1,000, 09-463) and BAF53a (1:1,000, 10341-1-AP) were from Millipore and Proteintech, respectively.

Techniques: Expressing, Transduction, Reporter Gene Assay, Construct, Binding Assay, Transfection, Immunoprecipitation, Western Blot, Activity Assay, Staining, Two Tailed Test