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  • 97
    Thermo Fisher hibernate a
    Hibernate A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 231 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hibernate a - by Bioz Stars, 2020-05
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    93
    Millipore poly d lysine laminin
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Poly D Lysine Laminin, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Cell Signaling Technology Inc 4oc
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    4oc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Becton Dickinson 4oc
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    4oc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    4oc  (Avantor)
    89
    Avantor 4oc
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    4oc, supplied by Avantor, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Merck KGaA pre chilled 4oc absolute ethanol
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Pre Chilled 4oc Absolute Ethanol, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    4oc  (Abcam)
    99
    Abcam 4oc
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    4oc, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Eppendorf AG eppendorf 5417r centrifuge
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Eppendorf 5417r Centrifuge, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 411 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Agilent technologies fluorescent mounting medium
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Fluorescent Mounting Medium, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 2744 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore polyvinylidene fluoride pvdf membrane
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Polyvinylidene Fluoride Pvdf Membrane, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6813 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore mgcl2
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Mgcl2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 39277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore protein g sepharose beads
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Protein G Sepharose Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3056 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Becton Dickinson phosflow perm buffer iii
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Phosflow Perm Buffer Iii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 96/100, based on 1013 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare protein asepharose fast flow
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Protein Asepharose Fast Flow, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GE Healthcare akta fplc apparatus
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Akta Fplc Apparatus, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore dna conjugated beads
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Dna Conjugated Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore collagen
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Collagen, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Beckman Coulter microfuge 20r
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Microfuge 20r, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    GE Healthcare ge illustra rnaspin isolation kit
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Ge Illustra Rnaspin Isolation Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    LifeSensors tube2 agarose beads
    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
    Tube2 Agarose Beads, supplied by LifeSensors, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
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    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on <t>poly-D-lysine/laminin</t> coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P
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    NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on poly-D-lysine/laminin coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P

    Journal: Molecular Therapy

    Article Title: Neural Stem Cells Engineered to Express Three Therapeutic Factors Mediate Recovery from Chronic Stage CNS Autoimmunity

    doi: 10.1038/mt.2016.104

    Figure Lengend Snippet: NT-3/IL-10/LINGO-1-Fc promotes NSC proliferation and differentiation in vitro . ( a ) NSCs transduced with NT-3/IL-10/LINGO-1-Fc were plated on poly-D-lysine/laminin coated coverslips at a density of 2.0 × 10 4 cell/ml in 24-well plates and cultured in NSC proliferation medium. After 48 hours of culture with Dox (1.5 μg/ml), cells were stained with anti-Ki67 antibody. ( b ) Proliferation of NSCs was quantified as the percentage of Ki67-positive cells in all GFP-positive cells. ( c ) Examples of NSCs that differentiated into mature oligodendrocytes (MBP + ), neurons (NF-H + ), and astrocytes (GFAP + ) in specific differentiation medium for 2 weeks. Scale bar = 100 µm. ( d ) Quantitative analysis of differentiated cells. Data are shown as mean values ± SD ( n = 5 each group) and are representative of three experiments. Groups designated by the same letter are not significantly different, while those with different letters (A, B, C, D, E, or F) are significantly different ( P

    Article Snippet: Cells were plated on poly-D-lysine/laminin (Sigma-Aldrich, St. Louis, MO) coated 24-well plate and cultured in serum-free Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12) (Invitrogen, Gaithersberg, MD) supplemented with 2% B27, 20 ng/ml epidermal growth factor, and 20 ng/ml basic fibroblast growth factor along with antibiotics.

    Techniques: In Vitro, Transduction, Cell Culture, Staining