Journal: Scientific Reports
Article Title: A fast and cost-effective method for apolipoprotein E isotyping as an alternative to APOE genotyping for patient screening and stratification
Figure Lengend Snippet: Binding of apoE4 to previously “blocked” ELISA plates. Recombinant apoE4 (dark gray bars), plasma from an APOE ε3/ε4 donor (light grey bars) or PBS (open bars) was allowed to interact with ELISA plate wells previously treated with different suitable blocking solutions: PBS (PBS with no blocking solution), BSA (0.25% BSA solution in 15 mM borate buffer containing 100 mM NaCl, pH 8.5), Superblock (Superblock T20 (ThermoFisher Scientific)), Polysorbate 20 (1% polysorbate 20 in PBS), Triton X-100 (1% Triton X-100 in PBS), Skim milk (5% skim milk in PBS containing 0.1% polysorbate 20), ODGP (8 mM Octyl b-D-glucopyranoside in PBS. Error bars represent the standard deviation of duplicated measures performed in two independent experiments.
Article Snippet: After washing, mouse monoclonal antibody specific for apoE4 (clone 4E4, Novus Biologicals, #NBP1-49529) diluted 1:2,000 in TBS-T plus 20% blocking solution, was added onto the wells and incubated for 45 minutes at RT.
Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Recombinant, Blocking Assay, Standard Deviation