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Journal: FEMS microbiology ecology
Article Title: Gastrointestinal microbial community shifts observed following oral administration of a Lactobacillus fermentum strain to mice.
doi: 10.1111/j.1574-6941.2003.tb01052.x
Figure Lengend Snippet: Fig. 2. Relationship between intensity of ‘lactobacilli’ band in the DGGE pro¢les generated from resolution of 16S rDNA ampli¢ed from faecal samples and total numbers of viable Lactobacillus spp. deter- mined by enumeration of colony-forming units on Rogosa agar. Faecal samples utilised were collected from mouse 1 following dosing with L. fermentum KLD. a: Changes in intensity of DGGE ‘lactobacilli’ band corresponding to ampli¢ed 16S V3 rDNA regions from faecal samples collected over sampling times. Lanes 1 and 2, pre-dose samples; lane 3, sample 46 h after ¢rst dose; lanes 4^13, samples taken at 4h (lane 4), 8 h (lane 5), 11 h (lane 6), 24 h (lane 7), 36 h (lane 8), 54 h (lane 9), 78 h (lane 10), 102 h (lane 11), 126 h (lane 12), 148 h (lane 13) after second dose; lane 14, 16S rDNA ampli¢ed from a pure culture of L. fermentum KLD. b: Changes in total host Lactobacillus numbers (CFU mg31 wet weight) in faeces over sampling time. Numbers on the graph correlate to the lanes in panel a and represent samples taken 4 h (4), 8 h (5), 11 h (6), 24 h (7), 36 h (8), 54 h (9), 78 h (10), 102 h (11), 126 h (12), 148 h (13) after the second dose.
Article Snippet: The following Lactobacillus strains were obtained from various culture collections: L. fermentum DSMZ 20391, 20052 (DSMZ; Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Germany), L. fermentum LMG 8896 (LMG; Laboratorium voor Microbiologie, Universiteit Gent, Belgium), L. fermentum BMF 30025, 30026 (BMF; Bundesanstalt fu«r Milchforschung, Kiel, Germany), Lactobacillus reuteri DSMZ 20016,
Techniques: Generated, Sampling