Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The Clinicopathological Characteristics of the Study Population

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques:

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The levels of serum AFP, GPC3, GP73 and DCP in each subgroup. ( A ) AFP. ( B ) GPC3. ( C ) GP73. ( D ) DCP. ( E ) Representative image of each subgroup. * P <0.05, ** P <0.01. Abbreviations: HCC, hepatocellular carcinoma; LC, liver cirrhosis; CHB, chronic hepatitis B virus infection; HC, healthy controls; AFP, α-fetoprotein; GPC3, glypican 3; GP73, golgi protein 73; DCP, des-γ-carboxy prothrombin; ns, no significance; int, intensity.

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques: Virus, Infection

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The Value of Serum AFP, GPC3, GP73 and DCP in the Diagnosis of HCC (Including All HCC Patients)

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques:

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: Assessment of the diagnostic value of serum AFP, GPC3, GP73 and DCP in differentiating HBV-related HCC from controls. ( A ) All HCC vs LC, CHB, HC. ( B ) All HCC vs LC, CHB. ( C ) All HCC v s LC. ( D ) Very early and early stage HCC vs LC, CHB, HC. ( E ) Very early and early stage HCC vs LC, CHB. ( F ) Very early and early stage HCC vs LC. Abbreviations: HCC, hepatocellular carcinoma; LC, liver cirrhosis; CHB, chronic hepatitis B virus infection; HC, healthy controls; AFP, α-fetoprotein; GPC3, glypican 3; GP73, golgi protein 73; DCP, des-γ-carboxy prothrombin.

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques: Diagnostic Assay, Virus, Infection

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The Value of Serum AFP, GPC3, GP73 and DCP in the Early Diagnosis of HCC (Including the Very Early HCC)

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques:

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The Value of Serum AFP, GPC3, GP73 and DCP in the Very Early Diagnosis of HCC

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques:

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The value of serum AFP, GPC3, GP73 and DCP in the very early diagnosis of HBV-related HCC. ( A ) Very early stage HCC vs LC, CHB, HC. ( B ) Very early stage vs LC, CHB. ( C ) Very early stage vs LC. Abbreviations: HCC, hepatocellular carcinoma; LC, liver cirrhosis; CHB, chronic hepatitis B virus infection; HC, healthy controls; AFP, α-fetoprotein; GPC3, glypican 3; GP73, golgi protein 73; DCP, des-γ-carboxy prothrombin.

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques: Virus, Infection

Journal: The Journal of Cell Biology

Article Title: Sarcospan-dependent Akt activation is required for utrophin expression and muscle regeneration

doi: 10.1083/jcb.201110032

Figure Lengend Snippet: SSPN increases expression of utrophin, dystrophin, and integrin. (A–D) Transverse cryosections of quadriceps muscles were stained with indicated antibodies. Bar, 50 µm. (E) Levels of utrophin were quantified by densitometry of protein bands from immunoblotting of quadriceps protein samples. Values are expressed relative to mdx levels (100%). Error bars represent standard deviation of the mean ( n = 3 mice per genotype). Statistical significance of the difference between samples was determined by Student’s t test (*, P < 0.05). (F) Quantitative RT-PCR was used to investigate whether overexpression of SSPN altered RNA levels of utrophin (Utr). RNA was isolated from mdx , mdx 1.5 , and mdx 3.0 skeletal muscle. mRNA expression levels were normalized to GAPDH. Data are expressed relative to that of mdx controls. Error bars represent standard deviation. Utrophin mRNA levels in Akt transgenic mdx mice were identical to mdx . A.U., arbitrary unit; Intg, integrin; Dys, dystrophin; hSSPN, human SSPN.

Article Snippet: Incubations were performed with the following primary antibodies: dystrophin (MANDYS1; 1:2), utrophin (MANCHO3; 1:50), α-DG IIH6 (sc-53987; 1:500; Santa Cruz Biotechnology, Inc.), β-DG (MANDAG2; 1:250), α-SG (VP-A105; 1:100), β-SG (VP-B206; 1:100), γ-SG (VP-G803; 1:100), laminin (L9393; 1:5,000), β1D integrin (MAB1900; 1:100), α7A integrin (gift from D.J.

Techniques: Expressing, Muscles, Staining, Western Blot, Standard Deviation, Quantitative RT-PCR, Over Expression, Isolation, Transgenic Assay

Journal: The Journal of Cell Biology

Article Title: Sarcospan-dependent Akt activation is required for utrophin expression and muscle regeneration

doi: 10.1083/jcb.201110032

Figure Lengend Snippet: SSPN facilitates transportation of the UGC to the sarcolemma to restore laminin binding. (A) Transverse cryosections of quadriceps muscles from the indicated mice were stained with antilaminin antibodies. Bar, 50 µm. (B) Skeletal muscles were solubilized in RIPA buffer, and 50 µg of each protein lysate was resolved by SDS-PAGE. Immunoblotting was performed with indicated antibodies. GAPDH and Coomassie blue (CB) loading controls are shown in Fig. S1 . (C and D) sWGA-enriched protein samples were analyzed for laminin binding. Laminin overlays (Lam O/L) represent binding to immobilized α-DG on nitrocellulose transfers. Blots were also stained with antibodies to α- and β-DG as indicated. (E) Proteins in ER and Golgi membranes (ER/Golgi) were purified from mdx , mdx 1.5 , and mdx 3.0 muscles. Protein samples were resolved by SDS-PAGE and subjected to immunoblotting with the indicated antibodies. Staining with endogenous (mouse SSPN [mSSPN]) and exogenous (human SSPN [hSSPN]) is shown. ATF6 and GM130 served as protein markers for the ER and Golgi, respectively. (F) Proteins in ER and Golgi membranes (ER/Golgi) were purified from mdx ( mdx ) and SSPN-deficient mdx ( mdx :SSPN −/− ) muscles and analyzed by immunoblotting. WFA binding to α-DG was assessed using WFA ligand overlay assays (WFA O/L). Lam, laminin; O/L, overlay; Agr, agrin; PItn, plectin-1; Dys, dystrophin; FL, full length; Utr, utrophin; Intg, integrin.

Article Snippet: Incubations were performed with the following primary antibodies: dystrophin (MANDYS1; 1:2), utrophin (MANCHO3; 1:50), α-DG IIH6 (sc-53987; 1:500; Santa Cruz Biotechnology, Inc.), β-DG (MANDAG2; 1:250), α-SG (VP-A105; 1:100), β-SG (VP-B206; 1:100), γ-SG (VP-G803; 1:100), laminin (L9393; 1:5,000), β1D integrin (MAB1900; 1:100), α7A integrin (gift from D.J.

Techniques: Binding Assay, Muscles, Staining, SDS Page, Western Blot, Purification

Journal: The Journal of Cell Biology

Article Title: Sarcospan-dependent Akt activation is required for utrophin expression and muscle regeneration

doi: 10.1083/jcb.201110032

Figure Lengend Snippet: SSPN is required for activation of Akt signaling. (A) Quadriceps protein lysates were prepared in modified RIPA buffer, and 50-µg samples were analyzed by immunoblotting as shown. GAPDH and Coomassie blue (CB) staining are provided as controls for equal loading. (B) Total skeletal muscle from wild-type (WT) and SSPN-null (SSPN −/− ) mice were solubilized in modified RIPA buffer, and 60 µg of each sample was analyzed by immunoblotting with indicated antibodies. GAPDH is provided as a control for equal loading. (C) Skeletal muscle protein lysates from WT, mdx , utrophin-deficient mdx muscle ( mdx :utr −/− ), and α7 integrin–deficient mdx muscle ( mdx : α7 −/− ) were enriched by sWGA lectin affinity chromatography (sWGA eluate). Immunoblots of 10-µg bound proteins eluted with GlcNAc are shown. Void (unbound) fractions are shown in Fig. S5 B . (D) WT quadriceps were injected with CTX to induce skeletal muscle degeneration/regeneration to evaluate the expression of utrophin and WFA binding without the complications of mdx pathology. Injected muscle cryosections were costained with utrophin (green fluorescence) and embryonic myosin heavy chain (eMHC; red fluorescence) as a marker for newly regenerated fibers 4 d after CTX injection. Serial sections were stained with WFA lectin (green fluorescence). Staining was visualized by indirect immunofluorescence. Actively regenerating myofibers displayed robust utrophin expression and WFA binding around the same nonjunctional areas of the sarcolemma. Mice were 6.5 wk of age at the time of analysis. Bar, 50 μm. Utr, utrophin; Dys, dystrophin; P, phospho; Intg, integrin.

Article Snippet: Incubations were performed with the following primary antibodies: dystrophin (MANDYS1; 1:2), utrophin (MANCHO3; 1:50), α-DG IIH6 (sc-53987; 1:500; Santa Cruz Biotechnology, Inc.), β-DG (MANDAG2; 1:250), α-SG (VP-A105; 1:100), β-SG (VP-B206; 1:100), γ-SG (VP-G803; 1:100), laminin (L9393; 1:5,000), β1D integrin (MAB1900; 1:100), α7A integrin (gift from D.J.

Techniques: Activation Assay, Modification, Western Blot, Staining, Control, Affinity Chromatography, Injection, Expressing, Binding Assay, Fluorescence, Marker, Immunofluorescence