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pcmv intron myc rab6 t27n  (Addgene inc)
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Addgene inc pcmv intron myc rab6 t27n
The overlap of giantin and Rab6a during Golgi biogenesis. ( A ) Confocal immunofluorescence images of Rab6a in HeLa cells after 60 min of BFA-WO, pretreated with scramble, giantin, GM130, or GRASP65 siRNAs. All confocal images acquired with the same imaging parameters; bars, 10 μm. ( B ) Quantification of cells with membranous Rab6a in cells presented in ( A ); n = 90 cells from three independent experiments, results are expressed as a mean ± SD; * p < 0.001. ( C ) Giantin immunostaining in DMSO- and BFA-treated HeLa cells. ( D ) Giantin immunostaining in HeLa cells after 60 min of BFA-WO, transfected with scramble, Rab6a siRNAs, and dominant-negative (GDP-bound) <t>Rab6a(T27N).</t> ( E ) Rab6a W-B of lysates of HeLa cells treated with corresponding siRNAs; β-actin was a loading control. ( F ) Quantifications of cells with perinuclear Golgi in cells from ( C , D ); n = 90 cells from three independent experiments, results expressed as a mean ± SD; *, p < 0.001.
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The overlap of giantin and Rab6a during Golgi biogenesis. ( A ) Confocal immunofluorescence images of Rab6a in HeLa cells after 60 min of BFA-WO, pretreated with scramble, giantin, GM130, or GRASP65 siRNAs. All confocal images acquired with the same imaging parameters; bars, 10 μm. ( B ) Quantification of cells with membranous Rab6a in cells presented in ( A ); n = 90 cells from three independent experiments, results are expressed as a mean ± SD; * p < 0.001. ( C ) Giantin immunostaining in DMSO- and BFA-treated HeLa cells. ( D ) Giantin immunostaining in HeLa cells after 60 min of BFA-WO, transfected with scramble, Rab6a siRNAs, and dominant-negative (GDP-bound) Rab6a(T27N). ( E ) Rab6a W-B of lysates of HeLa cells treated with corresponding siRNAs; β-actin was a loading control. ( F ) Quantifications of cells with perinuclear Golgi in cells from ( C , D ); n = 90 cells from three independent experiments, results expressed as a mean ± SD; *, p < 0.001.

Journal: Cells

Article Title: Post-ER Stress Biogenesis of Golgi Is Governed by Giantin

doi: 10.3390/cells8121631

Figure Lengend Snippet: The overlap of giantin and Rab6a during Golgi biogenesis. ( A ) Confocal immunofluorescence images of Rab6a in HeLa cells after 60 min of BFA-WO, pretreated with scramble, giantin, GM130, or GRASP65 siRNAs. All confocal images acquired with the same imaging parameters; bars, 10 μm. ( B ) Quantification of cells with membranous Rab6a in cells presented in ( A ); n = 90 cells from three independent experiments, results are expressed as a mean ± SD; * p < 0.001. ( C ) Giantin immunostaining in DMSO- and BFA-treated HeLa cells. ( D ) Giantin immunostaining in HeLa cells after 60 min of BFA-WO, transfected with scramble, Rab6a siRNAs, and dominant-negative (GDP-bound) Rab6a(T27N). ( E ) Rab6a W-B of lysates of HeLa cells treated with corresponding siRNAs; β-actin was a loading control. ( F ) Quantifications of cells with perinuclear Golgi in cells from ( C , D ); n = 90 cells from three independent experiments, results expressed as a mean ± SD; *, p < 0.001.

Article Snippet: PCMV-intron myc Rab6 T27N was a gift from Terry Hebert (Addgene, Cambridge, MA, USA, plasmid # 46782) [ ].

Techniques: Immunofluorescence, Imaging, Immunostaining, Transfection, Dominant Negative Mutation, Control