Journal: Scientific reports

Article Title: GLUT1-mediated HMGB1 O-GlcNAcylation drives hyperglycemia-Induced neutrophil extracellular trap networks formation via TLR4 signaling and exacerbates fibroblast inflammation.

doi: 10.1038/s41598-025-03642-z

Figure Lengend Snippet: Fig. 5. Detection of NETs-related molecules, JNK/p38 MAPK signalling pathway expression. (a) Free DNA quantification and ELISA to detect cfDNA, MPO-DNA and Cit-H3 expression levels. (b) The phosphorylation level of JNK, p-38 protein was determined by western blot. *P < 0.05, **P < 0.01, ***P < 0.001. n = 3, the experiment was repeated 3 times. JNK, c-Jun N-terminal kinase; MAPK, mitogen-activated protein kinase; p, phosphorylated.

Article Snippet: SAPK/JNK Antibody #9252, Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668, p38 MAPK Antibody #9212, Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 (CST, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Phospho-proteomics, Western Blot

Journal: Journal of biochemical and molecular toxicology

Article Title: Pyridostigmine bromide, chlorpyrifos, and DEET combined Gulf War exposure insult depresses mitochondrial function in neuroblastoma cells

doi: 10.1002/jbt.22913

Figure Lengend Snippet: Antibody information

Article Snippet: Analysis was performed using Image Lab Version 5.2 after normalization to total protein on the stain-free membrane. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Primary antibody Antigen Species, types Catalog info pSAPK p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185 Rabbit, monoclonal Cell Signaling: 4668T P38 MAPK p38 MAPK only when phosphorylated at Thr180 and Tyr182 Rabbit, monoclonal Cell Signaling: 4511T Secondary antibody Species, types Catalog info Goat anti rabbit biotinylated Santa Cruz Bio: sc2054 Open in a separate window Antibody information 2.6 |.

Techniques:

Journal: Journal of biochemical and molecular toxicology

Article Title: Pyridostigmine bromide, chlorpyrifos, and DEET combined Gulf War exposure insult depresses mitochondrial function in neuroblastoma cells

doi: 10.1002/jbt.22913

Figure Lengend Snippet: P38/MAPK and pSAPK/JNK are proapoptotic cell signaling stress-responsive kinases. 1, 2, and 4 h incubation with 0.01, 0.1, and 1.0× GWI does not cause proapoptotic signaling (A–C). 8 h incubation with 1.0× GWI induces p38/MAPK signaling (D1) and 0.1 and 1.0× GWI induce pSAPK/JNK signaling (D2). White asterisk (*) indicates nonspecific band and white arrow indicates a specific band. GWI, Gulf War Illness

Article Snippet: Analysis was performed using Image Lab Version 5.2 after normalization to total protein on the stain-free membrane. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Primary antibody Antigen Species, types Catalog info pSAPK p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185 Rabbit, monoclonal Cell Signaling: 4668T P38 MAPK p38 MAPK only when phosphorylated at Thr180 and Tyr182 Rabbit, monoclonal Cell Signaling: 4511T Secondary antibody Species, types Catalog info Goat anti rabbit biotinylated Santa Cruz Bio: sc2054 Open in a separate window Antibody information 2.6 |.

Techniques: Incubation