Journal: Cell Reports Medicine

Article Title: CAN-Scan: A multi-omic phenotype-driven precision oncology platform identifies prognostic biomarkers of therapy response for colorectal cancer

doi: 10.1016/j.xcrm.2025.102053

Figure Lengend Snippet:

Article Snippet: Tamoxifen citrate , MedChemExpress , Cat# HY-13757.

Techniques: Recombinant, Red Blood Cell Lysis, Sequencing, Software

Journal: Frontiers in Cell and Developmental Biology

Article Title: Estrogen-induced FXR1 promotes endocrine resistance and bone metastasis in breast cancer via BCL2 and GPX4

doi: 10.3389/fcell.2025.1563353

Figure Lengend Snippet: Estrogen induces FXR1 translation via eIF4E and eIF4EBP1. (a) , Immunoblot assessment of FXR1 levels. Cells were treated with 10 nM estrogen for 0h, 12h, 24h and 48h. (b) , Immunoblot assessment of FXR1 levels. Cells were treated with estrogen (10 nM) in combination with tamoxifen (1 μM) and fulvestrant (1 μM) for 48 h. (c) , Immunoblot assessment of FXR1 and ERα levels in ESR1 depleted MCF-7 and T47D cells. (d) , Immunoblot assessment of FXR1 and ERα levels in MDA-MB-231 cells with forced expression of ESR1 and control cells. (e) , Representative polysome traces of MCF-7 cells in estrogen-deprived and stimulated (10 nM) conditions. Distance (mm) 10-30: mRNA ribonucleo protein (mRNP)/monosome; distance (mm) 30-50: light polysome (LMW); distance (mm) 50-70: heavy polysome (HMW). (f) , Relative abundance of FXR1 mRNA in LMW or HMW shown in (e) . (g) , Representative polysome traces of MCF-7 cells after DMSO or fulvestrant (1 μM) treatment 48h. (h) , Relative abundance of FXR1 mRNA in LMW or HMW shown in (g) . (i) , Immunoblot assessment of FXR1 and eIF4E levels. MCF-7 and T47D sh-Control or sh-eIF4E cell lines were treated with estrogen (10 nM) for 48 h. (j, k) , Immunoblot assessment of FXR1, eIF4E, eIF4EBP1 and phosphorylated eIF4EBP1 levels. MCF-7 and T47D cells were treated with different concentrations of rapamycin (j) or combined with estrogen (k) . (l, m) , Immunoblot assessment of FXR1, eIF4E, eIF4EBP1 and phosphorylated eIF4EBP1 protein levels. In the presence or absence of estrogen, MCF-7, and T47D cells were treated with different concentrations of ZSTK474 (l) or AKT inhibitors (m) . (n) , Immunoblot assessment of FXR1 and IGF1R levels in IGF1R depleted MCF-7, T47D, and control cells. (o) , Schematic diagram of the pathway that estrogen and IGF1 regulate FXR1 translation. Results are shown as mean ± S.D. *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant (Unpaired two-tailed Student’s t test).

Article Snippet: MCF-7 TAMR and T47D TAMR cell lines were derived from parental cells maintained in culture with 1 μM tamoxifen (TargetMol, United States; T6906) for at least 6 months.

Techniques: Western Blot, Expressing, Control, Two Tailed Test

Journal: Frontiers in Cell and Developmental Biology

Article Title: Estrogen-induced FXR1 promotes endocrine resistance and bone metastasis in breast cancer via BCL2 and GPX4

doi: 10.3389/fcell.2025.1563353

Figure Lengend Snippet: FXR1 enhances oncogenicity of breast cancer cells. (a) , qPCR (n = 3 biological replicates) and immunoblot analysis of FXR1 expression in FXR1 depleted MCF-7 and control cells. (b) , Foci formation assay was performed in FXR1 depleted MCF-7 and control cells. Representative images (left) and statistical analyses (right) of the colonies were shown. (c) , MTT assay showing relative cell viability in FXR1 depleted MCF-7 and control cells. (d) , Statistical analyses of the FXR1 depleted MCF-7 cell colonies in 3D culture and soft agar colony formation assays. (e) , Early apoptotic population (FITC+/PerCP-Cy5.5-) in FXR1 depleted MCF-7 cells was determined by flow cytometry. (f) , Statistical analyses of the apoptotic FXR1 depleted MCF-7, T47D, and BT474 cells by flow cytometry. (g) , Immunoblot assessment of apoptosis-associated marker levels in FXR1 depleted MCF-7, T47D, and control cells. (h–k) , FXR1 depleted MCF-7 and T47D cells rescued with empty vector or R-FXR1 plasmid . Immunoblot assessment of FXR1 levels (h) , MTT assay showing relative cell viability (i, j) , and cell apoptosis determined by flow cytometry (k) . (l) , Immunoblot assessment of FXR1 levels after forced expression of FXR1 MCF-7 and T47D cells. (m, n) , Foci formation assay was performed in MCF-7 ( m ) and T47D (n) cells with forced expression of FXR1. (o, p) , MTT assay showing relative cell viability in MCF-7 (o) and T47D (p) cells with forced expression of FXR1. (q) , Early apoptotic population in FXR1-deleted MCF-7 and T47D cells was determined by flow cytometry. (r) , MTT assay showing relative cell viability in FXR1-deletion MCF-7 and control cells. (s–u) , MCF-7 cells stably expressing pLKO1 or shFXR1 plasmid were injected into nude mice (n = 8/group). Tumor size was measured starting at 7 days after injection. The tumor picture (s) , tumor growth curves (t) , and tumor weight (u ) was shown. Results are shown as mean ± S.D. *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant (Unpaired two-tailed Student’s t test in (m, n, q, u) , one-way ANOVA test in (a, b, d, f, k) others two-way ANOVA test.).

Article Snippet: MCF-7 TAMR and T47D TAMR cell lines were derived from parental cells maintained in culture with 1 μM tamoxifen (TargetMol, United States; T6906) for at least 6 months.

Techniques: Western Blot, Expressing, Control, Tube Formation Assay, MTT Assay, Flow Cytometry, Marker, Plasmid Preparation, Stable Transfection, Injection, Two Tailed Test

Journal: Frontiers in Cell and Developmental Biology

Article Title: Estrogen-induced FXR1 promotes endocrine resistance and bone metastasis in breast cancer via BCL2 and GPX4

doi: 10.3389/fcell.2025.1563353

Figure Lengend Snippet: FXR1 depletion promotes ferroptosis. (a, b) , Viability of FXR1 depleted MCF-7 (a) and BT474 (b) cells treated with 5 µM Z-VAD-FMK, 1 mM 3-MA, 1 µM NEC1 or 2 µM FER1 was determined. (c–e) , Lipid peroxidation was assessed by flow cytometry after C11-BODIPY staining in FXR1 depleted MCF-7 (c) , BT474 (d) and T47D (e) cells. Representative flow cytometry images (left) and statistical analyses (right) were shown. (f–h) , Relative GSH and GSSH levels were measured in FXR1 depleted MCF-7 (f) , T47D (g) and BT474 (h) cells. (i, j) , Transmission electron microscopy images show mitochondrial morphology in FXR1 depleted MCF-7 (i) , BT474 (j) , and control cells. White arrows indicate the mitochondria. (k–n) , MTT assays detect the sensitivity of FXR1 depleted MCF-7, T47D, and control cells to erastin or RSL3. Results are shown as mean ± S.D. *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant (Two-way ANOVA test in (k–n) others one-way ANOVA test.).

Article Snippet: MCF-7 TAMR and T47D TAMR cell lines were derived from parental cells maintained in culture with 1 μM tamoxifen (TargetMol, United States; T6906) for at least 6 months.

Techniques: Flow Cytometry, Staining, Transmission Assay, Electron Microscopy, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: Estrogen-induced FXR1 promotes endocrine resistance and bone metastasis in breast cancer via BCL2 and GPX4

doi: 10.3389/fcell.2025.1563353

Figure Lengend Snippet: FXR1 interacts with the GPX4 mRNA to regulate ferroptosis. (a, b) , Viability (a) and death (b) of FXR1 depleted MCF-7 cells were detected, treated with 10 µM erastin or 1 µM RSL3 combined with 2 µM FER1, 1 µM LIP1 or 5 µM DFO. (c) , Heat map analysis of RNA-seq data showing mRNA expression of ferroptosis-related genes in FXR1 depleted cells compared to control cells. (d, e) , qPCR (n = 3 biological replicates) and immunoblot analysis of GPX4 expression in FXR1 depleted MCF-7 (d) and T47D (e) cells. (f, g) , qPCR (n = 3 biological replicates) analyzed the interaction of FXR1 with GPX4 mRNA by RIP assays in MCF-7 (f) and T47D (g) cells. (h) , Schematic representation of luciferase reporter plasmids containing full-length and mutated GPX4-3′UTR (up). FXR1 recognition motif predicted by RBPsuite was shown (down). (i) , Luciferase reporter plasmids were co-transfected with FXR1 expression plasmid or vector control in HEK-293T cells, and luciferase activities were determined. (j, k) , FXR1 depleted MCF-7 (j) and T47D (k) cells were treated with actinomycin (d) . GPX4 mRNA were examined at the indicated time points. (l) , Schematic diagram of full-length and domain mutated of FXR1. (m, n) , Flag-FL, Flag-KH, or Flag-RGG were transfected in HEK-293T cells for RIP assays using Flag antibody. Immunoblot (m) and qPCR (n = 3 biological replicates) (n) were performed to analyze the association of different FXR1 domains with GPX4 mRNA. (o) , Immunoblot assessment of GPX4 levels in FXR1-depletion MCF-7 and T47D cells rescued with empty vector or GPX4 plasmid. (p, q) , Lipid peroxidation was assessed in FXR1 depleted MCF-7 (p) and T47D (q) cells rescued with empty vector or GPX4 plasmid. Results are shown as mean ± S.D. *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant (Unpaired two-tailed Student’s t test in (f, g, i) , two-way ANOVA test in (j, k) others one-way ANOVA test.).

Article Snippet: MCF-7 TAMR and T47D TAMR cell lines were derived from parental cells maintained in culture with 1 μM tamoxifen (TargetMol, United States; T6906) for at least 6 months.

Techniques: RNA Sequencing, Expressing, Control, Western Blot, Luciferase, Transfection, Plasmid Preparation, Two Tailed Test

Journal: Frontiers in Cell and Developmental Biology

Article Title: Estrogen-induced FXR1 promotes endocrine resistance and bone metastasis in breast cancer via BCL2 and GPX4

doi: 10.3389/fcell.2025.1563353

Figure Lengend Snippet: FXR1 promotes anti-estrogen resistance and bone metastasis. (a) , Immunoblot assessment of FXR1 levels in TAMR, LTED and cognate parental cells. (b, c) , The sensitivity of FXR1 depleted MCF-7 TAMR (b) and T47D TAMR (c) cells to tamoxifen was evaluated by MTT assays. (d) , FXR1 depleted MCF-7 TAMR cells were treated with 5 µM tamoxifen, 10 µM erastin, or both, and foci formation assays were performed. (e, f) , MCF-7 TAMR cells stably expressing pLKO1 or sh-FXR1 vector were injected into nude mice (n = 8/group). After the tumor size reached 150 mm 3 , the mice were treated with tamoxifen and IKE. Tumor growth curves (e) and tumor weight (f) are shown. (g) , Representative images of bioluminescence (BLI) and micro-CT of bone metastasis through caudal artery injection of 1.5 × 10 6 MCF-7 TAMR-shControl or -shFXR1 cells into nude mice (n = 5/group). BLI quantification of bone metastases in nude mice is shown on the right. (h) , Representative immunohistochemical images of TRAP and H&E staining were taken in each group. Scale bars: 50 μm. (i) , Foci formation assay was performed by using FXR1 depleted MCF-7 TAMR and T47D TAMR cells treated with 1 µM fulvestrant, 10 µM erastin, or both. (j, k) , Cell viability was measured by MTT assay by using FXR1 depleted MCF-7 TAMR (j) and T47D TAMR cells (k) treated with fulvestrant and erastin. (l) , Representative images of BLI and micro-CT of bone metastasis through caudal artery injection of 2 × 10 6 MCF-7 TAMR cells into nude mice. The mice were treated with vehicle, fulvestrant, IKE, or fulvestrant + IKE. (m) , BLI quantification of bone metastases in nude mice from (l) . (n) , Representative TRAP, H&E, and immunostaining images of FXR1, BCL2, GPX4, Ki67, TUNEL, and 4-HNE were shown from sections of (l) . Scale bars: 50 µm. Results are shown as mean ± S.D. *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant (Unpaired two-tailed Student’s t test in (g) , two-way ANOVA test in (b, c and e) others one-way ANOVA test.).

Article Snippet: MCF-7 TAMR and T47D TAMR cell lines were derived from parental cells maintained in culture with 1 μM tamoxifen (TargetMol, United States; T6906) for at least 6 months.

Techniques: Western Blot, Stable Transfection, Expressing, Plasmid Preparation, Injection, Micro-CT, Immunohistochemical staining, Staining, Tube Formation Assay, MTT Assay, Immunostaining, TUNEL Assay, Two Tailed Test

Journal: Viruses

Article Title: Antiviral Activity of Selective Estrogen Receptor Modulators against Severe Fever with Thrombocytopenia Syndrome Virus In Vitro and In Vivo

doi: 10.3390/v16081332

Figure Lengend Snippet: Anti-severe fever with thrombocytopenia syndrome virus activity and cytotoxicity of selective estrogen receptor modulators in Huh7 cells.

Article Snippet: Lasoxifene tartrate, Amcenestrant, Brilanestrant, Clomiphene citrate, Fulvestrant, Cyclofenil, Chlorotrianisene, Tamoxifen citrate, Estrio, and Pipendoxifene HCl were purchased from TargetMol Chemicals (Shanghai, China).

Techniques: Virus, Activity Assay