Journal: Science Progress

Article Title: Roles of the CDCA gene family in breast carcinoma

doi: 10.1177/00368504241312305

Figure Lengend Snippet: Prediction of TFs of CDCAs promoters. (a) Results of EPD and PROMO databases; (b) GEPIA showing up-regulation of FOXP3 and YY1 in BRCA in comparison of normal tissues; (c) correlation between CDCAs and FOXP3 expression in GEPIA; (d) correlation between CDCAs and YY1 expression in GEPIA. * P < 0.05.

Article Snippet: FOXP3 siRNAs (sc-43569), YY1 siRNAs (sc-36863), and control siRNA (sc-37007) were purchased from Santa Cruz Biotechnology (Santa Cruz, USA).

Techniques: Comparison, Expressing

Journal: Science Progress

Article Title: Roles of the CDCA gene family in breast carcinoma

doi: 10.1177/00368504241312305

Figure Lengend Snippet: Expressions of FOXP3 and YY1 in BRCA samples through IHC: (a) microscopic observation (100×); (b) comparison of FOXP3 and YY1 expression scores between breast tumors and normal tissues ( n = 70); (c) correlation between CDCAs and FOXP3 expression in BRCA samples; (d) correlation between CDCAs and YY1 expression in BRCA samples. *** P < 0.001.

Article Snippet: FOXP3 siRNAs (sc-43569), YY1 siRNAs (sc-36863), and control siRNA (sc-37007) were purchased from Santa Cruz Biotechnology (Santa Cruz, USA).

Techniques: Comparison, Expressing

Journal: Science Progress

Article Title: Roles of the CDCA gene family in breast carcinoma

doi: 10.1177/00368504241312305

Figure Lengend Snippet: Regulation of CDCAs by silencing FOXP3 and YY1:. (a) qRT-PCR investigating the decrease of FOXP3 and YY1 mRNA via siRNAs; (b) Western blotting analysis detecting expressions of FOXP3, YY1 and CDCAs proteins. *** P < 0.001.

Article Snippet: FOXP3 siRNAs (sc-43569), YY1 siRNAs (sc-36863), and control siRNA (sc-37007) were purchased from Santa Cruz Biotechnology (Santa Cruz, USA).

Techniques: Quantitative RT-PCR, Western Blot

Journal: Journal of Translational Medicine

Article Title: Amelioration of autoimmune arthritis by adoptive transfer of Foxp3-expressing regulatory B cells is associated with the Treg/Th17 cell balance

doi: 10.1186/s12967-016-0940-7

Figure Lengend Snippet: In vitro generation of regulatory B cells with suppressor activity by transfection of Foxp3. Splenic CD19 + B cells from DBA/1J mice were transfected with Foxp3 shRNA, a Foxp3 over-expression construct, or control plasmid DNA, and then stimulated with 10 µg/ml LPS, or 10 µg/ml anti-IgM for 72 h. a Relative Foxp3 mRNA levels in Foxp3 shRNA or Foxp3 over-expressing cells were determined by RT-PCR using primers specific for Foxp3 or β-actin. The relative quantity of Foxp3 was normalized to that of β-actin in each sample. b Cell viability of CD19 + B cells by MTT assay 24 h after plasmid transfection. c Suppression of the proliferation of responder CD4 + CD25 − T cell stimulation with anti-CD3 by B cells. CD4 + CD25 − T cells isolated from the spleens of CIA mice were cultured with Foxp3 shRNA or Foxp3-transfected CD19 + B cells in the absence ( left panel ) or presence ( right panel ) of Cll. Proliferation was determined after 3 days of culture by 3 H-thymidine incorporation. Cultures were harvested and cpm were determined. Values are the mean ± SD from triplicate cultures. Data represent one of the two independent mice

Article Snippet: Foxp3-specific targeting short hairpin RNA (shRNA) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: In Vitro, Activity Assay, Transfection, shRNA, Over Expression, Construct, Control, Plasmid Preparation, Expressing, Reverse Transcription Polymerase Chain Reaction, MTT Assay, Cell Stimulation, Isolation, Cell Culture