454 Search Results


dr marco fiocchetti  (ATCC)
94
ATCC dr marco fiocchetti
Dr Marco Fiocchetti, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dr marco fiocchetti/product/ATCC
Average 94 stars, based on 1 article reviews
dr marco fiocchetti - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
c tetani atcc 454  (ATCC)
94
ATCC c tetani atcc 454
C Tetani Atcc 454, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c tetani atcc 454/product/ATCC
Average 94 stars, based on 1 article reviews
c tetani atcc 454 - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
cpla2 antibody  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology cpla2 antibody
FIG. 3. Integrin-dependent phosphorylation of <t>cPLA2</t> and re- lease of arachidonic acid metabolites are Ras-dependent. A, lysates prepared from parental (3T3) or N17Ras-expressing (DN2) NIH3T3 cells were prepared as described in the legend to Fig. 2. 10 mg of lysate were electrophoresed and cPLA2 activation was analyzed by immunoblotting with a cPLA2 antibody to detect the mobility shift. The slower migrating band indicates the activated (phosphorylated) form of cPLA2 (cPLA2P). B, [3H]arachidonic acid-labeled cells were washed and plated onto fibronectin- (FN) or polylysine- (pL) coated plates, and the release of [3H]arachidonic acid was assayed as described under “Exper- imental Procedures.” The data shown represent the average of tripli- cate samples 6 S.D., from a typical experiment conducted three times.
Cpla2 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cpla2 antibody/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
cpla2 antibody - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
advanced mirna hsa mir 454 5p 478919 mir  (Thermo Fisher)
86
Thermo Fisher advanced mirna hsa mir 454 5p 478919 mir
FIG. 3. Integrin-dependent phosphorylation of <t>cPLA2</t> and re- lease of arachidonic acid metabolites are Ras-dependent. A, lysates prepared from parental (3T3) or N17Ras-expressing (DN2) NIH3T3 cells were prepared as described in the legend to Fig. 2. 10 mg of lysate were electrophoresed and cPLA2 activation was analyzed by immunoblotting with a cPLA2 antibody to detect the mobility shift. The slower migrating band indicates the activated (phosphorylated) form of cPLA2 (cPLA2P). B, [3H]arachidonic acid-labeled cells were washed and plated onto fibronectin- (FN) or polylysine- (pL) coated plates, and the release of [3H]arachidonic acid was assayed as described under “Exper- imental Procedures.” The data shown represent the average of tripli- cate samples 6 S.D., from a typical experiment conducted three times.
Advanced Mirna Hsa Mir 454 5p 478919 Mir, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/advanced mirna hsa mir 454 5p 478919 mir/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
advanced mirna hsa mir 454 5p 478919 mir - by Bioz Stars, 2026-03
86/100 stars
  Buy from Supplier
cd4 t cell isolation kit  (Miltenyi Biotec)
99
Miltenyi Biotec cd4 t cell isolation kit
FIG. 3. Integrin-dependent phosphorylation of <t>cPLA2</t> and re- lease of arachidonic acid metabolites are Ras-dependent. A, lysates prepared from parental (3T3) or N17Ras-expressing (DN2) NIH3T3 cells were prepared as described in the legend to Fig. 2. 10 mg of lysate were electrophoresed and cPLA2 activation was analyzed by immunoblotting with a cPLA2 antibody to detect the mobility shift. The slower migrating band indicates the activated (phosphorylated) form of cPLA2 (cPLA2P). B, [3H]arachidonic acid-labeled cells were washed and plated onto fibronectin- (FN) or polylysine- (pL) coated plates, and the release of [3H]arachidonic acid was assayed as described under “Exper- imental Procedures.” The data shown represent the average of tripli- cate samples 6 S.D., from a typical experiment conducted three times.
Cd4 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd4 t cell isolation kit/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
cd4 t cell isolation kit - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
liquichektm qualitative urine toxicology control  (Bio-Rad)
85
Bio-Rad liquichektm qualitative urine toxicology control
FIG. 3. Integrin-dependent phosphorylation of <t>cPLA2</t> and re- lease of arachidonic acid metabolites are Ras-dependent. A, lysates prepared from parental (3T3) or N17Ras-expressing (DN2) NIH3T3 cells were prepared as described in the legend to Fig. 2. 10 mg of lysate were electrophoresed and cPLA2 activation was analyzed by immunoblotting with a cPLA2 antibody to detect the mobility shift. The slower migrating band indicates the activated (phosphorylated) form of cPLA2 (cPLA2P). B, [3H]arachidonic acid-labeled cells were washed and plated onto fibronectin- (FN) or polylysine- (pL) coated plates, and the release of [3H]arachidonic acid was assayed as described under “Exper- imental Procedures.” The data shown represent the average of tripli- cate samples 6 S.D., from a typical experiment conducted three times.
Liquichektm Qualitative Urine Toxicology Control, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/liquichektm qualitative urine toxicology control/product/Bio-Rad
Average 85 stars, based on 1 article reviews
liquichektm qualitative urine toxicology control - by Bioz Stars, 2026-03
85/100 stars
  Buy from Supplier
sucrose  (ProSci Incorporated)
96
ProSci Incorporated sucrose
FIG. 3. Integrin-dependent phosphorylation of <t>cPLA2</t> and re- lease of arachidonic acid metabolites are Ras-dependent. A, lysates prepared from parental (3T3) or N17Ras-expressing (DN2) NIH3T3 cells were prepared as described in the legend to Fig. 2. 10 mg of lysate were electrophoresed and cPLA2 activation was analyzed by immunoblotting with a cPLA2 antibody to detect the mobility shift. The slower migrating band indicates the activated (phosphorylated) form of cPLA2 (cPLA2P). B, [3H]arachidonic acid-labeled cells were washed and plated onto fibronectin- (FN) or polylysine- (pL) coated plates, and the release of [3H]arachidonic acid was assayed as described under “Exper- imental Procedures.” The data shown represent the average of tripli- cate samples 6 S.D., from a typical experiment conducted three times.
Sucrose, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sucrose/product/ProSci Incorporated
Average 96 stars, based on 1 article reviews
sucrose - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
noggin  (Miltenyi Biotec)
95
Miltenyi Biotec noggin
FIG. 3. Integrin-dependent phosphorylation of <t>cPLA2</t> and re- lease of arachidonic acid metabolites are Ras-dependent. A, lysates prepared from parental (3T3) or N17Ras-expressing (DN2) NIH3T3 cells were prepared as described in the legend to Fig. 2. 10 mg of lysate were electrophoresed and cPLA2 activation was analyzed by immunoblotting with a cPLA2 antibody to detect the mobility shift. The slower migrating band indicates the activated (phosphorylated) form of cPLA2 (cPLA2P). B, [3H]arachidonic acid-labeled cells were washed and plated onto fibronectin- (FN) or polylysine- (pL) coated plates, and the release of [3H]arachidonic acid was assayed as described under “Exper- imental Procedures.” The data shown represent the average of tripli- cate samples 6 S.D., from a typical experiment conducted three times.
Noggin, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/noggin/product/Miltenyi Biotec
Average 95 stars, based on 1 article reviews
noggin - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
rea control rea293 recombinant human igg1  (Miltenyi Biotec)
97
Miltenyi Biotec rea control rea293 recombinant human igg1
FIG. 3. Integrin-dependent phosphorylation of <t>cPLA2</t> and re- lease of arachidonic acid metabolites are Ras-dependent. A, lysates prepared from parental (3T3) or N17Ras-expressing (DN2) NIH3T3 cells were prepared as described in the legend to Fig. 2. 10 mg of lysate were electrophoresed and cPLA2 activation was analyzed by immunoblotting with a cPLA2 antibody to detect the mobility shift. The slower migrating band indicates the activated (phosphorylated) form of cPLA2 (cPLA2P). B, [3H]arachidonic acid-labeled cells were washed and plated onto fibronectin- (FN) or polylysine- (pL) coated plates, and the release of [3H]arachidonic acid was assayed as described under “Exper- imental Procedures.” The data shown represent the average of tripli- cate samples 6 S.D., from a typical experiment conducted three times.
Rea Control Rea293 Recombinant Human Igg1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rea control rea293 recombinant human igg1/product/Miltenyi Biotec
Average 97 stars, based on 1 article reviews
rea control rea293 recombinant human igg1 - by Bioz Stars, 2026-03
97/100 stars
  Buy from Supplier
miltenyi biotec cd8a  (Miltenyi Biotec)
94
Miltenyi Biotec miltenyi biotec cd8a
FIG. 3. Integrin-dependent phosphorylation of <t>cPLA2</t> and re- lease of arachidonic acid metabolites are Ras-dependent. A, lysates prepared from parental (3T3) or N17Ras-expressing (DN2) NIH3T3 cells were prepared as described in the legend to Fig. 2. 10 mg of lysate were electrophoresed and cPLA2 activation was analyzed by immunoblotting with a cPLA2 antibody to detect the mobility shift. The slower migrating band indicates the activated (phosphorylated) form of cPLA2 (cPLA2P). B, [3H]arachidonic acid-labeled cells were washed and plated onto fibronectin- (FN) or polylysine- (pL) coated plates, and the release of [3H]arachidonic acid was assayed as described under “Exper- imental Procedures.” The data shown represent the average of tripli- cate samples 6 S.D., from a typical experiment conducted three times.
Miltenyi Biotec Cd8a, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/miltenyi biotec cd8a/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
miltenyi biotec cd8a - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
aza  (Chem Impex International)
95
Chem Impex International aza
FIG. 3. Integrin-dependent phosphorylation of <t>cPLA2</t> and re- lease of arachidonic acid metabolites are Ras-dependent. A, lysates prepared from parental (3T3) or N17Ras-expressing (DN2) NIH3T3 cells were prepared as described in the legend to Fig. 2. 10 mg of lysate were electrophoresed and cPLA2 activation was analyzed by immunoblotting with a cPLA2 antibody to detect the mobility shift. The slower migrating band indicates the activated (phosphorylated) form of cPLA2 (cPLA2P). B, [3H]arachidonic acid-labeled cells were washed and plated onto fibronectin- (FN) or polylysine- (pL) coated plates, and the release of [3H]arachidonic acid was assayed as described under “Exper- imental Procedures.” The data shown represent the average of tripli- cate samples 6 S.D., from a typical experiment conducted three times.
Aza, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/aza/product/Chem Impex International
Average 95 stars, based on 1 article reviews
aza - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
k racemifer dsm44963  (DSMZ)
88
DSMZ k racemifer dsm44963
FIG. 3. Integrin-dependent phosphorylation of <t>cPLA2</t> and re- lease of arachidonic acid metabolites are Ras-dependent. A, lysates prepared from parental (3T3) or N17Ras-expressing (DN2) NIH3T3 cells were prepared as described in the legend to Fig. 2. 10 mg of lysate were electrophoresed and cPLA2 activation was analyzed by immunoblotting with a cPLA2 antibody to detect the mobility shift. The slower migrating band indicates the activated (phosphorylated) form of cPLA2 (cPLA2P). B, [3H]arachidonic acid-labeled cells were washed and plated onto fibronectin- (FN) or polylysine- (pL) coated plates, and the release of [3H]arachidonic acid was assayed as described under “Exper- imental Procedures.” The data shown represent the average of tripli- cate samples 6 S.D., from a typical experiment conducted three times.
K Racemifer Dsm44963, supplied by DSMZ, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/k racemifer dsm44963/product/DSMZ
Average 88 stars, based on 1 article reviews
k racemifer dsm44963 - by Bioz Stars, 2026-03
88/100 stars
  Buy from Supplier

Image Search Results


FIG. 3. Integrin-dependent phosphorylation of cPLA2 and re- lease of arachidonic acid metabolites are Ras-dependent. A, lysates prepared from parental (3T3) or N17Ras-expressing (DN2) NIH3T3 cells were prepared as described in the legend to Fig. 2. 10 mg of lysate were electrophoresed and cPLA2 activation was analyzed by immunoblotting with a cPLA2 antibody to detect the mobility shift. The slower migrating band indicates the activated (phosphorylated) form of cPLA2 (cPLA2P). B, [3H]arachidonic acid-labeled cells were washed and plated onto fibronectin- (FN) or polylysine- (pL) coated plates, and the release of [3H]arachidonic acid was assayed as described under “Exper- imental Procedures.” The data shown represent the average of tripli- cate samples 6 S.D., from a typical experiment conducted three times.

Journal: The Journal of biological chemistry

Article Title: Ras activation is necessary for integrin-mediated activation of extracellular signal-regulated kinase 2 and cytosolic phospholipase A2 but not for cytoskeletal organization.

doi: 10.1074/jbc.271.25.14814

Figure Lengend Snippet: FIG. 3. Integrin-dependent phosphorylation of cPLA2 and re- lease of arachidonic acid metabolites are Ras-dependent. A, lysates prepared from parental (3T3) or N17Ras-expressing (DN2) NIH3T3 cells were prepared as described in the legend to Fig. 2. 10 mg of lysate were electrophoresed and cPLA2 activation was analyzed by immunoblotting with a cPLA2 antibody to detect the mobility shift. The slower migrating band indicates the activated (phosphorylated) form of cPLA2 (cPLA2P). B, [3H]arachidonic acid-labeled cells were washed and plated onto fibronectin- (FN) or polylysine- (pL) coated plates, and the release of [3H]arachidonic acid was assayed as described under “Exper- imental Procedures.” The data shown represent the average of tripli- cate samples 6 S.D., from a typical experiment conducted three times.

Article Snippet: The cPLA2 antibody was purchased from Santa Cruz Biotech.

Techniques: Phospho-proteomics, Expressing, Activation Assay, Western Blot, Mobility Shift, Labeling