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sirnas for simx1  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology sirnas for simx1
Figure 5. IFNα inhibits JEV replication in Mx-knockdown BHK-21 cells. Cells were transfected with siRNA targeting Mx1, Mx2, and a siRNA control (siCtrl). Six hours after transfection cells were treated with huIFNα for 12 h, then infected with JEV at an MOI of 0.05. At 24 hpi, cell supernatants were used to determine the levels of infectious virus by plaque assay, and the cell culture lysates were used to determine the viral protein levels by Western blot analysis. Percent knockdown of (A) Mx1 and (C) Mx2. JEV replication determined by plaque assay and Western blot analysis in (B) <t>siMx1-</t> or (D) siMx2-transfected and control cells. Quantification of the blotted proteins was performed using Image J software. All data are presented as means ± standard deviation (S.D.) as indicated. Statistical significance is indicated as ns (p > 0.05) and ** (p < 0.01).
Sirnas For Simx1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 5. IFNα inhibits JEV replication in Mx-knockdown BHK-21 cells. Cells were transfected with siRNA targeting Mx1, Mx2, and a siRNA control (siCtrl). Six hours after transfection cells were treated with huIFNα for 12 h, then infected with JEV at an MOI of 0.05. At 24 hpi, cell supernatants were used to determine the levels of infectious virus by plaque assay, and the cell culture lysates were used to determine the viral protein levels by Western blot analysis. Percent knockdown of (A) Mx1 and (C) Mx2. JEV replication determined by plaque assay and Western blot analysis in (B) siMx1- or (D) siMx2-transfected and control cells. Quantification of the blotted proteins was performed using Image J software. All data are presented as means ± standard deviation (S.D.) as indicated. Statistical significance is indicated as ns (p > 0.05) and ** (p < 0.01).

Journal: Viruses

Article Title: Mx Is Not Responsible for the Antiviral Activity of Interferon-α against Japanese Encephalitis Virus.

doi: 10.3390/v9010005

Figure Lengend Snippet: Figure 5. IFNα inhibits JEV replication in Mx-knockdown BHK-21 cells. Cells were transfected with siRNA targeting Mx1, Mx2, and a siRNA control (siCtrl). Six hours after transfection cells were treated with huIFNα for 12 h, then infected with JEV at an MOI of 0.05. At 24 hpi, cell supernatants were used to determine the levels of infectious virus by plaque assay, and the cell culture lysates were used to determine the viral protein levels by Western blot analysis. Percent knockdown of (A) Mx1 and (C) Mx2. JEV replication determined by plaque assay and Western blot analysis in (B) siMx1- or (D) siMx2-transfected and control cells. Quantification of the blotted proteins was performed using Image J software. All data are presented as means ± standard deviation (S.D.) as indicated. Statistical significance is indicated as ns (p > 0.05) and ** (p < 0.01).

Article Snippet: siRNA experiments were carried out in six-well plates containing BHK-21 cells or PK-15 cells starting at 2.5× 105 cells/well. siRNAs for siMx1 (sc-45260), siMx2 (sc-45261), and the negative-control siRNA (sc-37007) (Santa Cruz Biotechnology) were transfected at a concentration of 100 nM into cells using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions.

Techniques: Knockdown, Transfection, Control, Infection, Virus, Plaque Assay, Cell Culture, Western Blot, Software, Standard Deviation