Journal: Advanced Functional Materials

Article Title: Biomimetic Nanoparticles as a Theranostic Tool for Traumatic Brain Injury

doi: 10.1002/adfm.202100722

Figure Lengend Snippet: Nanoparticles’ (NPs) properties characterization. After fabrication, NPs were characterized for their physicochemical and biological properties using dynamic light scattering. No significant changes in A) size and B) polydispersity index (PDI) were observed. However, a significant decrease in C) zeta potential for leukosomes (Leuko) compared to liposomes (Lipo) was noticed. D) Representative cryo‐TEM images of Lipo and Leuko verified that no morphological changes occurred following membrane protein integration to the NPs. Scale bars = 100 nm. E) Western blots for leukocyte membrane protein markers: CD11b, CD18, CD47, and CD45 indicated their membrane integration in Leuko but absence in Lipo. Results are shown as mean ± SEM. Unpaired t‐test was used to determine statistical probabilities * p ≤ 0.05 among means considered statistically significant, n = 5.

Article Snippet: Antibodies for western blot rat anti‐CD11b (MAB11241), goat anti‐CD18 (AF2618), rabbit anti‐CD45 (EPR20033), goat anti‐CD47 (ab108415), anti‐rabbit IgG‐HRP, anti‐goat IgG‐HRP, and anti‐rat IgG (Bio‐Techne Corporation, Minnesota, USA).

Techniques: Zeta Potential Analyzer, Liposomes, Membrane, Western Blot

Journal: International Journal of Oncology

Article Title: Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells

doi: 10.3892/ijo.2016.3445

Figure Lengend Snippet: Sequences of the primer sets and the product sizes of RT-qPCR.

Article Snippet: Short interference RNA (siRNA) against podoplanin was purchased from Santa Cruz Biotechnology Inc. (TX, USA).

Techniques:

Journal: International Journal of Oncology

Article Title: Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells

doi: 10.3892/ijo.2016.3445

Figure Lengend Snippet: Endogenous expression of podoplanin as well as EMT-related markers in four of the SCC cell lines. Human esophageal squamous cell carcinoma TE-10, TE-11 and TE-5 cells were cultured to form a monolayer. A431 cells were used as a control. These cells were then lysed and the lysates were subjected to western blot analyses for podoplanin, EGFR, vimentin, N-cadherin, E-cadherin, Claudin-4, and actin (A). The same samples were used to examine DEC1 and DEC2 (B), thus the same internal control was used (B). One representative of three independent experiments with similar results is shown.

Article Snippet: Short interference RNA (siRNA) against podoplanin was purchased from Santa Cruz Biotechnology Inc. (TX, USA).

Techniques: Expressing, Cell Culture, Control, Western Blot

Journal: International Journal of Oncology

Article Title: Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells

doi: 10.3892/ijo.2016.3445

Figure Lengend Snippet: The mRNA expression of podoplanin and EMT-related markers was regulated by TGF-β. TE-11 cells and A431 cells were treated with TGF-β at 5.0 ng/ml for 24 h. The control cells were treated with TGF-β-diluted buffer. Total RNA was prepared and subjected to RT-qPCR for TGF-βRI, TGF-βRII, slug, podoplanin, vimentin, N-cadherin, E-cadherin, Claudin-4, DEC1, DEC2 and 18s rRNA. The data of podoplanin and 18s rRNA used in (A) was shown again in (B) as necessary. Two representative of at least four independent experiments with similar results are shown.

Article Snippet: Short interference RNA (siRNA) against podoplanin was purchased from Santa Cruz Biotechnology Inc. (TX, USA).

Techniques: Expressing, Control, Quantitative RT-PCR

Journal: International Journal of Oncology

Article Title: Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells

doi: 10.3892/ijo.2016.3445

Figure Lengend Snippet: The protein expression of podoplanin and EMT-related markers was regulated by TGF-β. TE-11 cells were treated as those indicated in . These cells were then lysed and the lysates were subjected to western blot analyses of pSmad2, Smad2/3, slug, podoplanin, vimentin, N-cadherin, E-cadherin, Claudin-4, DEC1, DEC2 and actin. Two representative of at least four independent experiments with similar results are shown.

Article Snippet: Short interference RNA (siRNA) against podoplanin was purchased from Santa Cruz Biotechnology Inc. (TX, USA).

Techniques: Expressing, Western Blot

Journal: International Journal of Oncology

Article Title: Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells

doi: 10.3892/ijo.2016.3445

Figure Lengend Snippet: TGF-β increases the expression of podoplanin, but decreases that of E-cadherin. (A) TE-11 and (B) A431 cells were cultured with 5.0 ng/ml of TGF-β for 24 h. The cells were then fixed, incubated with anti-podoplanin and anti-E-cadherin antibodies separately, and visualized using HRP-conjugated secondary antibody by DAB (brown). An image that is representative of at least two independent experiments with similar results is shown.

Article Snippet: Short interference RNA (siRNA) against podoplanin was purchased from Santa Cruz Biotechnology Inc. (TX, USA).

Techniques: Expressing, Cell Culture, Incubation

Journal: International Journal of Oncology

Article Title: Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells

doi: 10.3892/ijo.2016.3445

Figure Lengend Snippet: Upregulation of podoplanin by TGF-β is TGF-βRI/II-dependent. TE-11 cells were treated with the selectively inhibitor of TGF-β receptor SB431542 (1 or 10 μM) for 90 min before treated with or without TGF-β (5.0 ng/ml) for 24 h, and the lysates were subjected to western blot analyses of pSmad2, Smad2/3, slug, podoplanin, N-cadherin, E-cadherin, Claudin-4, and actin. One representative of at least three independent experiments with similar results is shown.

Article Snippet: Short interference RNA (siRNA) against podoplanin was purchased from Santa Cruz Biotechnology Inc. (TX, USA).

Techniques: Western Blot

Journal: International Journal of Oncology

Article Title: Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells

doi: 10.3892/ijo.2016.3445

Figure Lengend Snippet: Podoplanin knockdown inhibits cell proliferation in the presence and the absence of TGF-β (5.0 ng/ml) in TE-11 cells. (A) TE-11 cells cultured in 96-well plates were transfected with control siRNA or siRNA against podoplanin. At 24 h post-transfection, the cells were treated with or without TGF-β (5.0 ng/ml) and incubated for 24 h. The cell proliferation was measured by CCK-8 assay. The values are shown as a percentage of the control siRNA without TGF-β treatment. Each value represents the mean ± SEM (bars) of three independent experiments ( * P<0.001, compared with the control siRNA, according to the Student's t-test). (B) Podoplanin knockdown upregulated the expression of Claudin-4. TE-11 cells were treated as described above, and cell lysates were prepared and subjected to western blot analyses for the expression of podoplanin, Claudin-4, E-cadherin, and actin. One representative of at least three independent experiments with similar results is shown.

Article Snippet: Short interference RNA (siRNA) against podoplanin was purchased from Santa Cruz Biotechnology Inc. (TX, USA).

Techniques: Knockdown, Cell Culture, Transfection, Control, Incubation, CCK-8 Assay, Expressing, Western Blot

Journal: International Journal of Oncology

Article Title: Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells

doi: 10.3892/ijo.2016.3445

Figure Lengend Snippet: Podoplanin siRNA decreased the invasion induced by TGF-β in TE-11 cells. TE-11 cells were transfected with the control siRNA or podoplanin siRNA. At 24 h post-transfection, the cells were treated with or without TGF-β (5.0 ng/ml) and incubated for 24 h and 5×10 4 cells were seeded in the invasion chamber. At 48 h after seeding, the chambers were stained and the number of invasive cells was counted. The top panel shows a representative photograph, and the bottom panel shows the quantitative data. Each value represents the mean ± SEM (bars) of three independent experiments ( * P<0.001, compared with the control siRNA, according to the Student's t-test).

Article Snippet: Short interference RNA (siRNA) against podoplanin was purchased from Santa Cruz Biotechnology Inc. (TX, USA).

Techniques: Transfection, Control, Incubation, Staining

Journal: International Journal of Oncology

Article Title: Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells

doi: 10.3892/ijo.2016.3445

Figure Lengend Snippet: Podoplanin siRNA delayed the speed of wound-healing in TE-11 cells. TE-11 cells were transfected with control siRNA or siRNA against podoplanin for 24 h, a wound was made with a pipette tip and photographs of the wounded area were taken periodically. The remaining wound length was measured after 0, 24 and 48 h. The top panel shows representative photographs, and the bottom panel shows the quantitative data. Each value represents the mean ± SEM (bars) of three independent experiments ( * P<0.001, compared with the control siRNA, according to the Student's t-test).

Article Snippet: Short interference RNA (siRNA) against podoplanin was purchased from Santa Cruz Biotechnology Inc. (TX, USA).

Techniques: Transfection, Control, Transferring

Journal: International Journal of Oncology

Article Title: Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells

doi: 10.3892/ijo.2016.3445

Figure Lengend Snippet: Overexpression of (A) DEC1 and (B) DEC2 has different effects on podoplanin. TE-11 cells were transfected with the expression vector of DEC1 or DEC2 for 18 h, and cell lysates were prepared and subjected to western blot analyses for the expression of DEC1, DEC2, podoplanin, Claudin-4, and actin. One representative of at least three independent experiments with similar results is shown.

Article Snippet: Short interference RNA (siRNA) against podoplanin was purchased from Santa Cruz Biotechnology Inc. (TX, USA).

Techniques: Over Expression, Transfection, Expressing, Plasmid Preparation, Western Blot

Journal: Journal of thrombosis and haemostasis : JTH

Article Title: Inhibitory effects of polypeptides derived from a snake venom C-type lectin, aggretin, on tumor cell-induced platelet aggregation.

doi: 10.1111/jth.12519

Figure Lengend Snippet: Fig. 3. Podoplanin is expressed in various tumor cells and is involved in tumor-induced platelet aggregation. (A, B) Podoplanin is endogenously expressed in tumor cells as detected by western blotting with anti-podoplanin mAb; glyceraldehyde-3-phosphate dehydrogenase (GADPH) was used as an internal control. (C) Podoplanin knockdown in HuH-7 cells. Negative control (NC) and podoplanin (PDPN) small interfering RNA (siRNA)-transfected HuH-7 cells were analyzed for podoplanin expression by western blotting. (D) Podoplanin siRNA inhibited platelet aggregation of HuH-7 cells. Platelet aggregation was attenuated in podoplanin siR- NA-transfected HuH-7 cells. The results shown are representative of at least three similar experiments.

Article Snippet: Antibodies against human podoplanin and integrin b1, podoplanin small interfering RNA (siRNA) and control siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Western Blot, Control, Knockdown, Negative Control, Small Interfering RNA, Transfection, Expressing